Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19
Abstract Background Patients with COVID-19 can develop a range of immune responses, including variations in the onset and magnitude of antibody formation. The aim of this study was to investigate whether SARS-CoV-2 antibody levels vary in patients with mild to moderate COVID-19 in relation to the on...
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ftdoajarticles:oai:doaj.org/article:9a38dd860b1e49e2b59e24805908e053 2023-05-15T15:17:37+02:00 Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 Marwa M. Fekry Hanan Soliman Mona H. Hashish Heba S. Selim Nermin A. Osman Eman A. Omran 2023-03-01T00:00:00Z https://doi.org/10.1186/s42506-023-00131-x https://doaj.org/article/9a38dd860b1e49e2b59e24805908e053 EN eng SpringerOpen https://doi.org/10.1186/s42506-023-00131-x https://doaj.org/toc/2090-262X doi:10.1186/s42506-023-00131-x 2090-262X https://doaj.org/article/9a38dd860b1e49e2b59e24805908e053 Journal of the Egyptian Public Health Association, Vol 98, Iss 1, Pp 1-11 (2023) Seroconversion Anti-spike Anti-nucleocapsid SARS-CoV-2 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1186/s42506-023-00131-x 2023-03-26T01:33:34Z Abstract Background Patients with COVID-19 can develop a range of immune responses, including variations in the onset and magnitude of antibody formation. The aim of this study was to investigate whether SARS-CoV-2 antibody levels vary in patients with mild to moderate COVID-19 in relation to the onset (days) of their post-symptom seropositivity and to explore host factors that may affect antibody production Methods This was a prospective, multiple measurements study involving 92 PCR-confirmed patients with mild to moderate COVID-19. Antibody testing for anti-nucleocapsid (anti-NP) and spike proteins (anti-S) was performed using ELISA tests. Serum samples were collected over a period of 55 days from symptom onset of COVID-19 infection, and repeated as necessary until they turned positive. Results No significant differences were found between the positivity rates of anti-S or anti-NP regarding any clinical symptom (p > 0.05). The majority of patients who tested positive for anti-NP and anti-S showed early seropositivity (within 15 days of symptom onset) (75.9% for anti-NP and 82.6% for anti-S). Younger patients, those without chronic diseases, and non-healthcare workers had the highest percentage of seroconversion after day 35 post-symptom onset (p = 0.002, 0.028, and 0.036, respectively), while older patients and those with chronic diseases had earlier seropositivity and higher anti-NP levels (p = 0.003 and 0.06, respectively). Significantly higher anti-S ratios were found among older (p = 0.004), male (p = 0.015), and anemic patients (p = 0.02). A significant correlation was found between both antibodies (p = 0.001). At the end of the study, the cumulative seroconversion rate for both antibodies was almost 99%. Conclusions Some COVID-19 patients may exhibit delayed and weak immune responses, while elderly, anemic patients and those with chronic diseases may show earlier and higher antibody responses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of the Egyptian Public Health Association 98 1 |
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Seroconversion Anti-spike Anti-nucleocapsid SARS-CoV-2 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Seroconversion Anti-spike Anti-nucleocapsid SARS-CoV-2 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Marwa M. Fekry Hanan Soliman Mona H. Hashish Heba S. Selim Nermin A. Osman Eman A. Omran Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 |
topic_facet |
Seroconversion Anti-spike Anti-nucleocapsid SARS-CoV-2 Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract Background Patients with COVID-19 can develop a range of immune responses, including variations in the onset and magnitude of antibody formation. The aim of this study was to investigate whether SARS-CoV-2 antibody levels vary in patients with mild to moderate COVID-19 in relation to the onset (days) of their post-symptom seropositivity and to explore host factors that may affect antibody production Methods This was a prospective, multiple measurements study involving 92 PCR-confirmed patients with mild to moderate COVID-19. Antibody testing for anti-nucleocapsid (anti-NP) and spike proteins (anti-S) was performed using ELISA tests. Serum samples were collected over a period of 55 days from symptom onset of COVID-19 infection, and repeated as necessary until they turned positive. Results No significant differences were found between the positivity rates of anti-S or anti-NP regarding any clinical symptom (p > 0.05). The majority of patients who tested positive for anti-NP and anti-S showed early seropositivity (within 15 days of symptom onset) (75.9% for anti-NP and 82.6% for anti-S). Younger patients, those without chronic diseases, and non-healthcare workers had the highest percentage of seroconversion after day 35 post-symptom onset (p = 0.002, 0.028, and 0.036, respectively), while older patients and those with chronic diseases had earlier seropositivity and higher anti-NP levels (p = 0.003 and 0.06, respectively). Significantly higher anti-S ratios were found among older (p = 0.004), male (p = 0.015), and anemic patients (p = 0.02). A significant correlation was found between both antibodies (p = 0.001). At the end of the study, the cumulative seroconversion rate for both antibodies was almost 99%. Conclusions Some COVID-19 patients may exhibit delayed and weak immune responses, while elderly, anemic patients and those with chronic diseases may show earlier and higher antibody responses. |
format |
Article in Journal/Newspaper |
author |
Marwa M. Fekry Hanan Soliman Mona H. Hashish Heba S. Selim Nermin A. Osman Eman A. Omran |
author_facet |
Marwa M. Fekry Hanan Soliman Mona H. Hashish Heba S. Selim Nermin A. Osman Eman A. Omran |
author_sort |
Marwa M. Fekry |
title |
Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 |
title_short |
Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 |
title_full |
Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 |
title_fullStr |
Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 |
title_full_unstemmed |
Delayed seropositivity is associated with lower levels of SARS-CoV-2 antibody levels in patients with mild to moderate COVID-19 |
title_sort |
delayed seropositivity is associated with lower levels of sars-cov-2 antibody levels in patients with mild to moderate covid-19 |
publisher |
SpringerOpen |
publishDate |
2023 |
url |
https://doi.org/10.1186/s42506-023-00131-x https://doaj.org/article/9a38dd860b1e49e2b59e24805908e053 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of the Egyptian Public Health Association, Vol 98, Iss 1, Pp 1-11 (2023) |
op_relation |
https://doi.org/10.1186/s42506-023-00131-x https://doaj.org/toc/2090-262X doi:10.1186/s42506-023-00131-x 2090-262X https://doaj.org/article/9a38dd860b1e49e2b59e24805908e053 |
op_doi |
https://doi.org/10.1186/s42506-023-00131-x |
container_title |
Journal of the Egyptian Public Health Association |
container_volume |
98 |
container_issue |
1 |
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1766347848379531264 |