Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules
Patients with paracoccidioidomycosis often present pulmonary fibrosis and exhibit important respiratory limitations. Based on an already established animal model, the contribution of viable and non-viable P. brasiliensis propagules to the development of fibrosis was investigated. BALB/c male mice, 4...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:9a0fd945b5834899b2ced2d7c766b888 2024-09-09T19:27:51+00:00 Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules Ana M. COCK Luz E. CANO Diana VÉLEZ Beatriz H. ARISTIZÁBAL Judith TRUJILLO Angela RESTREPO 2000-04-01T00:00:00Z https://doaj.org/article/9a0fd945b5834899b2ced2d7c766b888 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000200001&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/9a0fd945b5834899b2ced2d7c766b888 Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 2, Pp 59-66 (2000) Pulmonary fibrosis Paracoccidioides brasiliensis Viable conidia Fragmented yeast cells Histopathology Collagen fibers Granuloma formation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2000 ftdoajarticles 2024-08-05T17:49:30Z Patients with paracoccidioidomycosis often present pulmonary fibrosis and exhibit important respiratory limitations. Based on an already established animal model, the contribution of viable and non-viable P. brasiliensis propagules to the development of fibrosis was investigated. BALB/c male mice, 4-6 weeks old were inoculated intranasally either with 4x10(6 )viable conidia (Group I), or 6.5x10(6) fragmented yeast cells (Group II). Control animals received PBS. Six mice per period were sacrificed at 24, 48, 72h (initial) and 1, 2, 4, 8, 12 and 16 weeks post-challenge (late). Paraffin embedded lungs were sectioned and stained with H&E, trichromic (Masson), reticulin and Grocott´s. During the initial period PMNs influx was important in both groups and acute inflammation involving 34% to 45% of the lungs was noticed. Later on, mononuclear cells predominated. In group I, the inflammation progressed and granulomas were formed and by the 12th week they fussed and became loose. Thick collagen I fibers were observed in 66.6% and 83.3% of the animals at 8 and 12 weeks, respectively. Collagen III, thick fibers became apparent in some animals at 4weeks and by 12 weeks, 83% of them exhibited alterations in the organization and thickness of these elements. In group II mice, this pattern was different with stepwise decrease in the number of inflammatory foci and lack of granulomas. Although initially most animals in this group had minor alterations in thin collagen I fibers, they disappeared by the 4th week. Results indicate that tissue response to fragmented yeast cells was transitory while viable conidia evoked a progressive inflammatory reaction leading to granuloma formation and to excess production and/or disarrangement of collagens I and III; the latter led to fibrosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Pulmonary fibrosis Paracoccidioides brasiliensis Viable conidia Fragmented yeast cells Histopathology Collagen fibers Granuloma formation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Pulmonary fibrosis Paracoccidioides brasiliensis Viable conidia Fragmented yeast cells Histopathology Collagen fibers Granuloma formation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Ana M. COCK Luz E. CANO Diana VÉLEZ Beatriz H. ARISTIZÁBAL Judith TRUJILLO Angela RESTREPO Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules |
topic_facet |
Pulmonary fibrosis Paracoccidioides brasiliensis Viable conidia Fragmented yeast cells Histopathology Collagen fibers Granuloma formation Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Patients with paracoccidioidomycosis often present pulmonary fibrosis and exhibit important respiratory limitations. Based on an already established animal model, the contribution of viable and non-viable P. brasiliensis propagules to the development of fibrosis was investigated. BALB/c male mice, 4-6 weeks old were inoculated intranasally either with 4x10(6 )viable conidia (Group I), or 6.5x10(6) fragmented yeast cells (Group II). Control animals received PBS. Six mice per period were sacrificed at 24, 48, 72h (initial) and 1, 2, 4, 8, 12 and 16 weeks post-challenge (late). Paraffin embedded lungs were sectioned and stained with H&E, trichromic (Masson), reticulin and Grocott´s. During the initial period PMNs influx was important in both groups and acute inflammation involving 34% to 45% of the lungs was noticed. Later on, mononuclear cells predominated. In group I, the inflammation progressed and granulomas were formed and by the 12th week they fussed and became loose. Thick collagen I fibers were observed in 66.6% and 83.3% of the animals at 8 and 12 weeks, respectively. Collagen III, thick fibers became apparent in some animals at 4weeks and by 12 weeks, 83% of them exhibited alterations in the organization and thickness of these elements. In group II mice, this pattern was different with stepwise decrease in the number of inflammatory foci and lack of granulomas. Although initially most animals in this group had minor alterations in thin collagen I fibers, they disappeared by the 4th week. Results indicate that tissue response to fragmented yeast cells was transitory while viable conidia evoked a progressive inflammatory reaction leading to granuloma formation and to excess production and/or disarrangement of collagens I and III; the latter led to fibrosis. |
format |
Article in Journal/Newspaper |
author |
Ana M. COCK Luz E. CANO Diana VÉLEZ Beatriz H. ARISTIZÁBAL Judith TRUJILLO Angela RESTREPO |
author_facet |
Ana M. COCK Luz E. CANO Diana VÉLEZ Beatriz H. ARISTIZÁBAL Judith TRUJILLO Angela RESTREPO |
author_sort |
Ana M. COCK |
title |
Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules |
title_short |
Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules |
title_full |
Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules |
title_fullStr |
Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules |
title_full_unstemmed |
Fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in BALB/c mice infected with viable and non-viable Paracoccidioides brasiliensis propagules |
title_sort |
fibrotic sequelae in pulmonary paracoccidioidomycosis: histopathological aspects in balb/c mice infected with viable and non-viable paracoccidioides brasiliensis propagules |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2000 |
url |
https://doaj.org/article/9a0fd945b5834899b2ced2d7c766b888 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 2, Pp 59-66 (2000) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000200001&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/9a0fd945b5834899b2ced2d7c766b888 |
_version_ |
1809897194188177408 |