Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA
Ongoing warming and sea-ice reductions in the Arctic can seriously impact cold-water species, such as polar cod (Boreogadus saida), necessitating biomonitoring to reveal the ecological consequences. Recent methodological advancements in environmental DNA (eDNA) techniques have increased our ability...
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Online Access: | https://doi.org/10.3389/fmars.2023.1193083 https://doaj.org/article/99fa3a4077c84b7eabd40e034ef62708 |
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ftdoajarticles:oai:doaj.org/article:99fa3a4077c84b7eabd40e034ef62708 2023-10-01T03:53:38+02:00 Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA Tatsuya Kawakami Aya Yamazaki Hai-Chao Jiang Hiromichi Ueno Akihide Kasai 2023-08-01T00:00:00Z https://doi.org/10.3389/fmars.2023.1193083 https://doaj.org/article/99fa3a4077c84b7eabd40e034ef62708 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2023.1193083/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2023.1193083 https://doaj.org/article/99fa3a4077c84b7eabd40e034ef62708 Frontiers in Marine Science, Vol 10 (2023) Arctic environmental DNA logistic regression analysis polar cod (Boreogadus saida) quantitative PCR sea ice Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2023 ftdoajarticles https://doi.org/10.3389/fmars.2023.1193083 2023-09-03T00:51:41Z Ongoing warming and sea-ice reductions in the Arctic can seriously impact cold-water species, such as polar cod (Boreogadus saida), necessitating biomonitoring to reveal the ecological consequences. Recent methodological advancements in environmental DNA (eDNA) techniques have increased our ability to conduct ecological monitoring at various locations, including the Arctic. This study aimed to provide an overview of the distribution of polar cod across the Bering and Chukchi Seas by employing species-specific detection of eDNA. First, we successfully developed novel species-specific qPCR assay targeting the mitochondrial D-loop region, which exclusively amplifies eDNA derived from polar cod. Subsequently, polar cod eDNA was detected using the assay from the samples that we collected latitudinally across the study area during the open-water season. Polar cod eDNA was primarily detected in the surface water from the central Chukchi Sea shelf and the northernmost observation line (75°N), which was located on the shelf slope, off the Point Barrow, and in the marginal ice zone. In contrast, only trace amounts of eDNA were detected in the Bering Sea. This pattern corresponded well with the distribution of water masses classified based on environmental conditions. The detection of eDNA in surface water was clearly limited to cold (-1 to 5°C) and low salinity (25–32) water, whereas it was detected in a higher salinity range (32–34) in the middle and bottom layers. These findings are consistent with current knowledge about the distribution and habitat of the polar cod, suggesting that eDNA can be regarded as a reliable tool to replace or supplement conventional methods. Incorporating eDNA techniques into large-scale oceanographic surveys can improve the spatial and temporal resolution of fish species detection with a reasonable sampling effort and will facilitate the continuous monitoring of Arctic ecosystems. Article in Journal/Newspaper Arctic Bering Sea Boreogadus saida Chukchi Chukchi Sea Point Barrow polar cod Sea ice Directory of Open Access Journals: DOAJ Articles Arctic Bering Sea Chukchi Sea Frontiers in Marine Science 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic environmental DNA logistic regression analysis polar cod (Boreogadus saida) quantitative PCR sea ice Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Arctic environmental DNA logistic regression analysis polar cod (Boreogadus saida) quantitative PCR sea ice Science Q General. Including nature conservation geographical distribution QH1-199.5 Tatsuya Kawakami Aya Yamazaki Hai-Chao Jiang Hiromichi Ueno Akihide Kasai Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA |
topic_facet |
Arctic environmental DNA logistic regression analysis polar cod (Boreogadus saida) quantitative PCR sea ice Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
Ongoing warming and sea-ice reductions in the Arctic can seriously impact cold-water species, such as polar cod (Boreogadus saida), necessitating biomonitoring to reveal the ecological consequences. Recent methodological advancements in environmental DNA (eDNA) techniques have increased our ability to conduct ecological monitoring at various locations, including the Arctic. This study aimed to provide an overview of the distribution of polar cod across the Bering and Chukchi Seas by employing species-specific detection of eDNA. First, we successfully developed novel species-specific qPCR assay targeting the mitochondrial D-loop region, which exclusively amplifies eDNA derived from polar cod. Subsequently, polar cod eDNA was detected using the assay from the samples that we collected latitudinally across the study area during the open-water season. Polar cod eDNA was primarily detected in the surface water from the central Chukchi Sea shelf and the northernmost observation line (75°N), which was located on the shelf slope, off the Point Barrow, and in the marginal ice zone. In contrast, only trace amounts of eDNA were detected in the Bering Sea. This pattern corresponded well with the distribution of water masses classified based on environmental conditions. The detection of eDNA in surface water was clearly limited to cold (-1 to 5°C) and low salinity (25–32) water, whereas it was detected in a higher salinity range (32–34) in the middle and bottom layers. These findings are consistent with current knowledge about the distribution and habitat of the polar cod, suggesting that eDNA can be regarded as a reliable tool to replace or supplement conventional methods. Incorporating eDNA techniques into large-scale oceanographic surveys can improve the spatial and temporal resolution of fish species detection with a reasonable sampling effort and will facilitate the continuous monitoring of Arctic ecosystems. |
format |
Article in Journal/Newspaper |
author |
Tatsuya Kawakami Aya Yamazaki Hai-Chao Jiang Hiromichi Ueno Akihide Kasai |
author_facet |
Tatsuya Kawakami Aya Yamazaki Hai-Chao Jiang Hiromichi Ueno Akihide Kasai |
author_sort |
Tatsuya Kawakami |
title |
Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA |
title_short |
Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA |
title_full |
Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA |
title_fullStr |
Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA |
title_full_unstemmed |
Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA |
title_sort |
distribution and habitat preference of polar cod (boreogadus saida) in the bering and chukchi seas inferred from species-specific detection of environmental dna |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fmars.2023.1193083 https://doaj.org/article/99fa3a4077c84b7eabd40e034ef62708 |
geographic |
Arctic Bering Sea Chukchi Sea |
geographic_facet |
Arctic Bering Sea Chukchi Sea |
genre |
Arctic Bering Sea Boreogadus saida Chukchi Chukchi Sea Point Barrow polar cod Sea ice |
genre_facet |
Arctic Bering Sea Boreogadus saida Chukchi Chukchi Sea Point Barrow polar cod Sea ice |
op_source |
Frontiers in Marine Science, Vol 10 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2023.1193083/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2023.1193083 https://doaj.org/article/99fa3a4077c84b7eabd40e034ef62708 |
op_doi |
https://doi.org/10.3389/fmars.2023.1193083 |
container_title |
Frontiers in Marine Science |
container_volume |
10 |
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1778520386243133440 |