Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes

Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured...

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Published in:Asian Pacific Journal of Tropical Biomedicine
Main Authors: Nurul Hikmah Harun, Abdi Wira Septama, Ibrahim Jantan
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2015
Subjects:
Online Access:https://doi.org/10.1016/S2221-1691(15)30170-2
https://doaj.org/article/9984007ae91248dc987e27671d7b4137
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spelling ftdoajarticles:oai:doaj.org/article:9984007ae91248dc987e27671d7b4137 2023-05-15T15:09:34+02:00 Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes Nurul Hikmah Harun Abdi Wira Septama Ibrahim Jantan 2015-01-01T00:00:00Z https://doi.org/10.1016/S2221-1691(15)30170-2 https://doaj.org/article/9984007ae91248dc987e27671d7b4137 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301702 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30170-2 https://doaj.org/article/9984007ae91248dc987e27671d7b4137 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 1, Pp 48-53 (2015) Selected Malaysian medicinal plants Immunomodulatory effects CD18/11a expression Phagocytosis activity Flow cytometry analysis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/S2221-1691(15)30170-2 2022-12-31T14:45:47Z Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 1 48 53
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Selected Malaysian medicinal plants
Immunomodulatory effects
CD18/11a expression
Phagocytosis activity
Flow cytometry analysis
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
spellingShingle Selected Malaysian medicinal plants
Immunomodulatory effects
CD18/11a expression
Phagocytosis activity
Flow cytometry analysis
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
Nurul Hikmah Harun
Abdi Wira Septama
Ibrahim Jantan
Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
topic_facet Selected Malaysian medicinal plants
Immunomodulatory effects
CD18/11a expression
Phagocytosis activity
Flow cytometry analysis
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
description Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.
format Article in Journal/Newspaper
author Nurul Hikmah Harun
Abdi Wira Septama
Ibrahim Jantan
author_facet Nurul Hikmah Harun
Abdi Wira Septama
Ibrahim Jantan
author_sort Nurul Hikmah Harun
title Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
title_short Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
title_full Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
title_fullStr Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
title_full_unstemmed Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
title_sort immunomodulatory effects of selected malaysian plants on the cd18/11a expression and phagocytosis activities of leukocytes
publisher Wolters Kluwer Medknow Publications
publishDate 2015
url https://doi.org/10.1016/S2221-1691(15)30170-2
https://doaj.org/article/9984007ae91248dc987e27671d7b4137
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 1, Pp 48-53 (2015)
op_relation http://www.sciencedirect.com/science/article/pii/S2221169115301702
https://doaj.org/toc/2221-1691
2221-1691
doi:10.1016/S2221-1691(15)30170-2
https://doaj.org/article/9984007ae91248dc987e27671d7b4137
op_doi https://doi.org/10.1016/S2221-1691(15)30170-2
container_title Asian Pacific Journal of Tropical Biomedicine
container_volume 5
container_issue 1
container_start_page 48
op_container_end_page 53
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