Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:9984007ae91248dc987e27671d7b4137 2023-05-15T15:09:34+02:00 Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes Nurul Hikmah Harun Abdi Wira Septama Ibrahim Jantan 2015-01-01T00:00:00Z https://doi.org/10.1016/S2221-1691(15)30170-2 https://doaj.org/article/9984007ae91248dc987e27671d7b4137 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301702 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30170-2 https://doaj.org/article/9984007ae91248dc987e27671d7b4137 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 1, Pp 48-53 (2015) Selected Malaysian medicinal plants Immunomodulatory effects CD18/11a expression Phagocytosis activity Flow cytometry analysis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/S2221-1691(15)30170-2 2022-12-31T14:45:47Z Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 1 48 53 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Selected Malaysian medicinal plants Immunomodulatory effects CD18/11a expression Phagocytosis activity Flow cytometry analysis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Selected Malaysian medicinal plants Immunomodulatory effects CD18/11a expression Phagocytosis activity Flow cytometry analysis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nurul Hikmah Harun Abdi Wira Septama Ibrahim Jantan Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes |
topic_facet |
Selected Malaysian medicinal plants Immunomodulatory effects CD18/11a expression Phagocytosis activity Flow cytometry analysis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system. |
format |
Article in Journal/Newspaper |
author |
Nurul Hikmah Harun Abdi Wira Septama Ibrahim Jantan |
author_facet |
Nurul Hikmah Harun Abdi Wira Septama Ibrahim Jantan |
author_sort |
Nurul Hikmah Harun |
title |
Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes |
title_short |
Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes |
title_full |
Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes |
title_fullStr |
Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes |
title_full_unstemmed |
Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes |
title_sort |
immunomodulatory effects of selected malaysian plants on the cd18/11a expression and phagocytosis activities of leukocytes |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/S2221-1691(15)30170-2 https://doaj.org/article/9984007ae91248dc987e27671d7b4137 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 1, Pp 48-53 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301702 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(15)30170-2 https://doaj.org/article/9984007ae91248dc987e27671d7b4137 |
op_doi |
https://doi.org/10.1016/S2221-1691(15)30170-2 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
1 |
container_start_page |
48 |
op_container_end_page |
53 |
_version_ |
1766340732191244288 |