Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice
Abstract Background Polar microalgae contain unique compounds that enable them to adapt to extreme environments. As the skin barrier is our first line of defense against external threats, polar microalgae extracts may possess restorative properties for damaged skin, but the potential of microalgae e...
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ftdoajarticles:oai:doaj.org/article:9910ac518bc7430d8a092afb7069f477 2023-08-20T04:02:34+02:00 Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice YoonHee Lim So-Hyun Park Eun Jae Kim HeeJun Lim Jinsun Jang In-Sun Hong Sanghee Kim YunJae Jung 2023-07-01T00:00:00Z https://doi.org/10.1186/s40659-023-00454-1 https://doaj.org/article/9910ac518bc7430d8a092afb7069f477 EN eng BMC https://doi.org/10.1186/s40659-023-00454-1 https://doaj.org/toc/0717-6287 doi:10.1186/s40659-023-00454-1 0717-6287 https://doaj.org/article/9910ac518bc7430d8a092afb7069f477 Biological Research, Vol 56, Iss 1, Pp 1-15 (2023) Polar microalgae Compound profiles Barrier protection Psoriasis Anti-inflammation Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.1186/s40659-023-00454-1 2023-07-30T00:40:07Z Abstract Background Polar microalgae contain unique compounds that enable them to adapt to extreme environments. As the skin barrier is our first line of defense against external threats, polar microalgae extracts may possess restorative properties for damaged skin, but the potential of microalgae extracts as skin protective agents remains unknown. Purpose This study aimed to analyze compound profiles from polar microalgae extracts, evaluate their potential as skin epithelial protective agents, and examine the underlying mechanisms. Methods Six different polar microalgae, Micractinium sp. (KSF0015 and KSF0041), Chlamydomonas sp. (KNM0029C, KSF0037, and KSF0134), and Chlorococcum sp. (KSF0003), were collected from the Antarctic or Arctic regions. Compound profiles of polar and non-polar microalgae extracts were analyzed using gas chromatography-mass spectrometry (GC-MS). The protective activities of polar microalgae extracts on human keratinocyte cell lines against oxidative stress, radiation, and psoriatic cytokine exposure were assessed. The potential anti-inflammatory mechanisms mediated by KSF0041, a polar microalga with protective properties against oxidative stress, ultraviolet (UV) B, and an inflammatory cytokine cocktail, were investigated using RNA-sequencing analysis. To evaluate the therapeutic activity of KSF0041, an imiquimod-induced murine model of psoriatic dermatitis was used. Results Polar microalgae contain components comparable to those of their non-polar counterparts, but also showed distinct differences, particularly in fatty acid composition. Polar microalgae extracts had a greater ability to scavenge free radicals than did non-polar microalgae and enhanced the viability of HaCaT cells, a human keratinocyte cell line, following exposure to UVB radiation or psoriatic cytokines. These extracts also reduced barrier integrity damage and decreased mRNA levels of inflammatory cytokines in psoriatic HaCaT cells. Treatment with KSF0041 extract altered the transcriptome of psoriatic HaCaT cells ... Article in Journal/Newspaper Antarc* Antarctic Arctic Directory of Open Access Journals: DOAJ Articles Antarctic Arctic The Antarctic Biological Research 56 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Polar microalgae Compound profiles Barrier protection Psoriasis Anti-inflammation Biology (General) QH301-705.5 |
spellingShingle |
Polar microalgae Compound profiles Barrier protection Psoriasis Anti-inflammation Biology (General) QH301-705.5 YoonHee Lim So-Hyun Park Eun Jae Kim HeeJun Lim Jinsun Jang In-Sun Hong Sanghee Kim YunJae Jung Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
topic_facet |
Polar microalgae Compound profiles Barrier protection Psoriasis Anti-inflammation Biology (General) QH301-705.5 |
description |
Abstract Background Polar microalgae contain unique compounds that enable them to adapt to extreme environments. As the skin barrier is our first line of defense against external threats, polar microalgae extracts may possess restorative properties for damaged skin, but the potential of microalgae extracts as skin protective agents remains unknown. Purpose This study aimed to analyze compound profiles from polar microalgae extracts, evaluate their potential as skin epithelial protective agents, and examine the underlying mechanisms. Methods Six different polar microalgae, Micractinium sp. (KSF0015 and KSF0041), Chlamydomonas sp. (KNM0029C, KSF0037, and KSF0134), and Chlorococcum sp. (KSF0003), were collected from the Antarctic or Arctic regions. Compound profiles of polar and non-polar microalgae extracts were analyzed using gas chromatography-mass spectrometry (GC-MS). The protective activities of polar microalgae extracts on human keratinocyte cell lines against oxidative stress, radiation, and psoriatic cytokine exposure were assessed. The potential anti-inflammatory mechanisms mediated by KSF0041, a polar microalga with protective properties against oxidative stress, ultraviolet (UV) B, and an inflammatory cytokine cocktail, were investigated using RNA-sequencing analysis. To evaluate the therapeutic activity of KSF0041, an imiquimod-induced murine model of psoriatic dermatitis was used. Results Polar microalgae contain components comparable to those of their non-polar counterparts, but also showed distinct differences, particularly in fatty acid composition. Polar microalgae extracts had a greater ability to scavenge free radicals than did non-polar microalgae and enhanced the viability of HaCaT cells, a human keratinocyte cell line, following exposure to UVB radiation or psoriatic cytokines. These extracts also reduced barrier integrity damage and decreased mRNA levels of inflammatory cytokines in psoriatic HaCaT cells. Treatment with KSF0041 extract altered the transcriptome of psoriatic HaCaT cells ... |
format |
Article in Journal/Newspaper |
author |
YoonHee Lim So-Hyun Park Eun Jae Kim HeeJun Lim Jinsun Jang In-Sun Hong Sanghee Kim YunJae Jung |
author_facet |
YoonHee Lim So-Hyun Park Eun Jae Kim HeeJun Lim Jinsun Jang In-Sun Hong Sanghee Kim YunJae Jung |
author_sort |
YoonHee Lim |
title |
Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
title_short |
Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
title_full |
Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
title_fullStr |
Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
title_full_unstemmed |
Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
title_sort |
polar microalgae extracts protect human hacat keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s40659-023-00454-1 https://doaj.org/article/9910ac518bc7430d8a092afb7069f477 |
geographic |
Antarctic Arctic The Antarctic |
geographic_facet |
Antarctic Arctic The Antarctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_source |
Biological Research, Vol 56, Iss 1, Pp 1-15 (2023) |
op_relation |
https://doi.org/10.1186/s40659-023-00454-1 https://doaj.org/toc/0717-6287 doi:10.1186/s40659-023-00454-1 0717-6287 https://doaj.org/article/9910ac518bc7430d8a092afb7069f477 |
op_doi |
https://doi.org/10.1186/s40659-023-00454-1 |
container_title |
Biological Research |
container_volume |
56 |
container_issue |
1 |
_version_ |
1774713074081595392 |