Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells.
The role of natural killer (NK) cells in infection-induced liver fibrosis remains obscure. In this study, we elucidated the effect of NK cells on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. Liver fibrosis was induced by infecting C57BL/6 mice with 18-20 cercariae of S. japonicum...
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ftdoajarticles:oai:doaj.org/article:98ffeaef6fdd46cc85e9f6bb4376b529 2023-05-15T15:11:11+02:00 Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. Xin Hou Fazhi Yu Suqin Man Dake Huang Yuxia Zhang Miao Liu Cuiping Ren Jijia Shen 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001456 https://doaj.org/article/98ffeaef6fdd46cc85e9f6bb4376b529 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3250498?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001456 https://doaj.org/article/98ffeaef6fdd46cc85e9f6bb4376b529 PLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1456 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001456 2022-12-31T02:31:54Z The role of natural killer (NK) cells in infection-induced liver fibrosis remains obscure. In this study, we elucidated the effect of NK cells on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. Liver fibrosis was induced by infecting C57BL/6 mice with 18-20 cercariae of S. japonicum. Anti-ASGM1 antibody was used to deplete NK cells. Toll-like receptor 3 ligand, polyinosinic-polycytidylic acid (poly I:C) was used to enhance the activation of NK cells. Results showed that NK cells were accumulated and activated after S. japonicum infection, as evidenced by the elevation of CD69 expression and IFN-γ production. Depletion of NK cells markedly enhanced S. japonicum egg-induced liver fibrosis. Administration of poly I:C further activated NK cells to produce IFN-γ and attenuated S. japonicum egg-induced liver fibrosis. The observed protective effect of poly I:C on liver fibrosis was diminished through depletion of NK cells. Disruption of IFN-γ gene enhanced liver fibrosis and partially abolished the suppression of liver fibrosis by poly I:C. Moreover, expression of retinoic acid early inducible 1 (RAE 1), the NKG2D ligand, was detectable at high levels on activated hepatic stellate cells derived from S. japonicum-infected mice, which made them more susceptible to hepatic NK cell killing. In conclusion, our findings suggest that the activated NK cells in the liver after S. japonicum infection negatively regulate egg-induced liver fibrosis via producing IFN-γ, and killing activated stellate cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rae ENVELOPE(-116.053,-116.053,62.834,62.834) PLoS Neglected Tropical Diseases 6 1 e1456 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xin Hou Fazhi Yu Suqin Man Dake Huang Yuxia Zhang Miao Liu Cuiping Ren Jijia Shen Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The role of natural killer (NK) cells in infection-induced liver fibrosis remains obscure. In this study, we elucidated the effect of NK cells on Schistosoma japonicum (S. japonicum) egg-induced liver fibrosis. Liver fibrosis was induced by infecting C57BL/6 mice with 18-20 cercariae of S. japonicum. Anti-ASGM1 antibody was used to deplete NK cells. Toll-like receptor 3 ligand, polyinosinic-polycytidylic acid (poly I:C) was used to enhance the activation of NK cells. Results showed that NK cells were accumulated and activated after S. japonicum infection, as evidenced by the elevation of CD69 expression and IFN-γ production. Depletion of NK cells markedly enhanced S. japonicum egg-induced liver fibrosis. Administration of poly I:C further activated NK cells to produce IFN-γ and attenuated S. japonicum egg-induced liver fibrosis. The observed protective effect of poly I:C on liver fibrosis was diminished through depletion of NK cells. Disruption of IFN-γ gene enhanced liver fibrosis and partially abolished the suppression of liver fibrosis by poly I:C. Moreover, expression of retinoic acid early inducible 1 (RAE 1), the NKG2D ligand, was detectable at high levels on activated hepatic stellate cells derived from S. japonicum-infected mice, which made them more susceptible to hepatic NK cell killing. In conclusion, our findings suggest that the activated NK cells in the liver after S. japonicum infection negatively regulate egg-induced liver fibrosis via producing IFN-γ, and killing activated stellate cells. |
format |
Article in Journal/Newspaper |
author |
Xin Hou Fazhi Yu Suqin Man Dake Huang Yuxia Zhang Miao Liu Cuiping Ren Jijia Shen |
author_facet |
Xin Hou Fazhi Yu Suqin Man Dake Huang Yuxia Zhang Miao Liu Cuiping Ren Jijia Shen |
author_sort |
Xin Hou |
title |
Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
title_short |
Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
title_full |
Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
title_fullStr |
Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
title_full_unstemmed |
Negative regulation of Schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
title_sort |
negative regulation of schistosoma japonicum egg-induced liver fibrosis by natural killer cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001456 https://doaj.org/article/98ffeaef6fdd46cc85e9f6bb4376b529 |
long_lat |
ENVELOPE(-116.053,-116.053,62.834,62.834) |
geographic |
Arctic Rae |
geographic_facet |
Arctic Rae |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 1, p e1456 (2012) |
op_relation |
http://europepmc.org/articles/PMC3250498?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001456 https://doaj.org/article/98ffeaef6fdd46cc85e9f6bb4376b529 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001456 |
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PLoS Neglected Tropical Diseases |
container_volume |
6 |
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1 |
container_start_page |
e1456 |
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