Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts.
RATIONALE:Chagas cardiomyopathy, caused by the protozoan Trypanosoma cruzi, is characterized by alterations in intracellular ion, heart failure and arrhythmias. Arrhythmias have been related to sudden death, even in asymptomatic patients, and their molecular mechanisms have not been fully elucidated...
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ftdoajarticles:oai:doaj.org/article:98fc907fd8ca41bdb8e4c68d0e578c80 2023-05-15T15:07:31+02:00 Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. Héctor O Rodríguez-Angulo Jhoan Toro-Mendoza Juan A Marques Juan L Concepción Rafael Bonfante-Cabarcas Yoliver Higuerey Luz E Thomas Leandro Balzano-Nogueira José R López Alfredo Mijares 2015-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003512 https://doaj.org/article/98fc907fd8ca41bdb8e4c68d0e578c80 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4315581?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003512 https://doaj.org/article/98fc907fd8ca41bdb8e4c68d0e578c80 PLoS Neglected Tropical Diseases, Vol 9, Iss 2, p e0003512 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003512 2022-12-31T04:11:06Z RATIONALE:Chagas cardiomyopathy, caused by the protozoan Trypanosoma cruzi, is characterized by alterations in intracellular ion, heart failure and arrhythmias. Arrhythmias have been related to sudden death, even in asymptomatic patients, and their molecular mechanisms have not been fully elucidated. OBJECTIVE:The aim of this study is to demonstrate the effect of proteins secreted by T. cruzi on healthy, isolated beating rat heart model under a non-damage-inducing protocol. METHODS AND RESULTS:We established a non-damage-inducing recirculation-reoxygenation model where ultrafiltrate fractions of conditioned medium control or conditioned infected medium were perfused at a standard flow rate and under partial oxygenation. Western blotting with chagasic patient serum was performed to determine the antigenicity of the conditioned infected medium fractions. We observed bradycardia, ventricular fibrillation and complete atrioventricular block in hearts during perfusion with >50 kDa conditioned infected culture medium. The preincubation of conditioned infected medium with chagasic serum abolished the bradycardia and arrhythmias. The proteins present in the conditioned infected culture medium of >50 kDa fractions were recognized by the chagasic patient sera associated with arrhythmias. CONCLUSIONS:These results suggest that proteins secreted by T. cruzi are involved in Chagas disease arrhythmias and may be a potential biomarker in chagasic patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 2 e0003512 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Héctor O Rodríguez-Angulo Jhoan Toro-Mendoza Juan A Marques Juan L Concepción Rafael Bonfante-Cabarcas Yoliver Higuerey Luz E Thomas Leandro Balzano-Nogueira José R López Alfredo Mijares Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
RATIONALE:Chagas cardiomyopathy, caused by the protozoan Trypanosoma cruzi, is characterized by alterations in intracellular ion, heart failure and arrhythmias. Arrhythmias have been related to sudden death, even in asymptomatic patients, and their molecular mechanisms have not been fully elucidated. OBJECTIVE:The aim of this study is to demonstrate the effect of proteins secreted by T. cruzi on healthy, isolated beating rat heart model under a non-damage-inducing protocol. METHODS AND RESULTS:We established a non-damage-inducing recirculation-reoxygenation model where ultrafiltrate fractions of conditioned medium control or conditioned infected medium were perfused at a standard flow rate and under partial oxygenation. Western blotting with chagasic patient serum was performed to determine the antigenicity of the conditioned infected medium fractions. We observed bradycardia, ventricular fibrillation and complete atrioventricular block in hearts during perfusion with >50 kDa conditioned infected culture medium. The preincubation of conditioned infected medium with chagasic serum abolished the bradycardia and arrhythmias. The proteins present in the conditioned infected culture medium of >50 kDa fractions were recognized by the chagasic patient sera associated with arrhythmias. CONCLUSIONS:These results suggest that proteins secreted by T. cruzi are involved in Chagas disease arrhythmias and may be a potential biomarker in chagasic patients. |
format |
Article in Journal/Newspaper |
author |
Héctor O Rodríguez-Angulo Jhoan Toro-Mendoza Juan A Marques Juan L Concepción Rafael Bonfante-Cabarcas Yoliver Higuerey Luz E Thomas Leandro Balzano-Nogueira José R López Alfredo Mijares |
author_facet |
Héctor O Rodríguez-Angulo Jhoan Toro-Mendoza Juan A Marques Juan L Concepción Rafael Bonfante-Cabarcas Yoliver Higuerey Luz E Thomas Leandro Balzano-Nogueira José R López Alfredo Mijares |
author_sort |
Héctor O Rodríguez-Angulo |
title |
Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. |
title_short |
Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. |
title_full |
Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. |
title_fullStr |
Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. |
title_full_unstemmed |
Evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by T. cruzi in isolated rat hearts. |
title_sort |
evidence of reversible bradycardia and arrhythmias caused by immunogenic proteins secreted by t. cruzi in isolated rat hearts. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003512 https://doaj.org/article/98fc907fd8ca41bdb8e4c68d0e578c80 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 2, p e0003512 (2015) |
op_relation |
http://europepmc.org/articles/PMC4315581?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003512 https://doaj.org/article/98fc907fd8ca41bdb8e4c68d0e578c80 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003512 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
2 |
container_start_page |
e0003512 |
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1766338998459957248 |