Molecular detection of drug resistant malaria in Southern Thailand
Abstract Background Drug resistance within the major malaria parasites Plasmodium vivax and Plasmodium falciparum threatens malaria control and elimination in Southeast Asia. Plasmodium vivax first-line treatment drug is chloroquine together with primaquine, and the first-line treatment for P. falci...
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ftdoajarticles:oai:doaj.org/article:98f2dd913a584335958be77b9ea85de6 2023-05-15T15:12:27+02:00 Molecular detection of drug resistant malaria in Southern Thailand Chaturong Noisang Christiane Prosser Wieland Meyer Waenurama Chemoh John Ellis Nongyao Sawangjaroen Rogan Lee 2019-08-01T00:00:00Z https://doi.org/10.1186/s12936-019-2903-y https://doaj.org/article/98f2dd913a584335958be77b9ea85de6 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2903-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2903-y 1475-2875 https://doaj.org/article/98f2dd913a584335958be77b9ea85de6 Malaria Journal, Vol 18, Iss 1, Pp 1-11 (2019) Drug resistance Molecular surveillance Southeast Asia Border malaria Plasmodium falciparum Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2903-y 2022-12-31T01:05:27Z Abstract Background Drug resistance within the major malaria parasites Plasmodium vivax and Plasmodium falciparum threatens malaria control and elimination in Southeast Asia. Plasmodium vivax first-line treatment drug is chloroquine together with primaquine, and the first-line treatment for P. falciparum malaria is artemisinin in combination with a partner drug. Plasmodium vivax and P. falciparum parasites resistant to their respective first-line therapies are now found within Southeast Asia. The resistance perimeters may include high transmission regions of Southern Thailand which are underrepresented in surveillance efforts. Methods This study investigated blood samples from malaria centres in Southern Thailand. Genetic loci associated with drug resistance were amplified and sequenced. Drug resistance associated genes Pvmdr1, Pvcrt-o, Pvdhfr, and Pvdhps were characterized for 145 cases of P. vivax malaria, as well as the artemisinin resistance-associated Pfkelch13 gene from 91 cases of P. falciparum malaria. Results Plasmodium vivax samples from Southern Thai provinces showed numerous chloroquine and antifolate resistance-associated mutations, including SNP and Pvcrt-o K10-insertion combinations suggestive of chloroquine resistant P. vivax phenotypes. A high proportion of the C580Y coding mutation (conferring artemisinin resistance) was detected in P. falciparum samples originating from Ranong and Yala (where the mutation was previously unreported). Conclusions The results demonstrate a risk of chloroquine and antifolate resistant P. vivax phenotypes in Southern Thailand, and artemisinin resistant P. falciparum observed as far south as the Thai–Malaysian border region. Ongoing surveillance of antimalarial drug resistance markers is called for in Southern Thailand to inform case management. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Drug resistance Molecular surveillance Southeast Asia Border malaria Plasmodium falciparum Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Drug resistance Molecular surveillance Southeast Asia Border malaria Plasmodium falciparum Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Chaturong Noisang Christiane Prosser Wieland Meyer Waenurama Chemoh John Ellis Nongyao Sawangjaroen Rogan Lee Molecular detection of drug resistant malaria in Southern Thailand |
topic_facet |
Drug resistance Molecular surveillance Southeast Asia Border malaria Plasmodium falciparum Plasmodium vivax Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Drug resistance within the major malaria parasites Plasmodium vivax and Plasmodium falciparum threatens malaria control and elimination in Southeast Asia. Plasmodium vivax first-line treatment drug is chloroquine together with primaquine, and the first-line treatment for P. falciparum malaria is artemisinin in combination with a partner drug. Plasmodium vivax and P. falciparum parasites resistant to their respective first-line therapies are now found within Southeast Asia. The resistance perimeters may include high transmission regions of Southern Thailand which are underrepresented in surveillance efforts. Methods This study investigated blood samples from malaria centres in Southern Thailand. Genetic loci associated with drug resistance were amplified and sequenced. Drug resistance associated genes Pvmdr1, Pvcrt-o, Pvdhfr, and Pvdhps were characterized for 145 cases of P. vivax malaria, as well as the artemisinin resistance-associated Pfkelch13 gene from 91 cases of P. falciparum malaria. Results Plasmodium vivax samples from Southern Thai provinces showed numerous chloroquine and antifolate resistance-associated mutations, including SNP and Pvcrt-o K10-insertion combinations suggestive of chloroquine resistant P. vivax phenotypes. A high proportion of the C580Y coding mutation (conferring artemisinin resistance) was detected in P. falciparum samples originating from Ranong and Yala (where the mutation was previously unreported). Conclusions The results demonstrate a risk of chloroquine and antifolate resistant P. vivax phenotypes in Southern Thailand, and artemisinin resistant P. falciparum observed as far south as the Thai–Malaysian border region. Ongoing surveillance of antimalarial drug resistance markers is called for in Southern Thailand to inform case management. |
format |
Article in Journal/Newspaper |
author |
Chaturong Noisang Christiane Prosser Wieland Meyer Waenurama Chemoh John Ellis Nongyao Sawangjaroen Rogan Lee |
author_facet |
Chaturong Noisang Christiane Prosser Wieland Meyer Waenurama Chemoh John Ellis Nongyao Sawangjaroen Rogan Lee |
author_sort |
Chaturong Noisang |
title |
Molecular detection of drug resistant malaria in Southern Thailand |
title_short |
Molecular detection of drug resistant malaria in Southern Thailand |
title_full |
Molecular detection of drug resistant malaria in Southern Thailand |
title_fullStr |
Molecular detection of drug resistant malaria in Southern Thailand |
title_full_unstemmed |
Molecular detection of drug resistant malaria in Southern Thailand |
title_sort |
molecular detection of drug resistant malaria in southern thailand |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2903-y https://doaj.org/article/98f2dd913a584335958be77b9ea85de6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-11 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2903-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2903-y 1475-2875 https://doaj.org/article/98f2dd913a584335958be77b9ea85de6 |
op_doi |
https://doi.org/10.1186/s12936-019-2903-y |
container_title |
Malaria Journal |
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18 |
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1 |
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1766343124246855680 |