Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses
ABSTRACT Small RNAs (sRNAs) play a very important role in gene regulation at the posttranscriptional level. However, sRNAs from nonmodel microorganisms, extremophiles in particular, have been rarely explored. We discovered a putative sRNA, termed Pf1 sRNA, in Pseudoalteromonas fuliginea BSW20308 iso...
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ftdoajarticles:oai:doaj.org/article:98e8825fe7ed44abb1b276705008f855 2023-05-15T15:14:13+02:00 Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses Jiao Wen Li Liao Zedong Duan Shiyuan Su Jin Zhang Bo Chen 2023-02-01T00:00:00Z https://doi.org/10.1128/spectrum.04094-22 https://doaj.org/article/98e8825fe7ed44abb1b276705008f855 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/spectrum.04094-22 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.04094-22 2165-0497 https://doaj.org/article/98e8825fe7ed44abb1b276705008f855 Microbiology Spectrum, Vol 11, Iss 1 (2023) Pseudoalteromonas sRNA CsrA temperature regulation Microbiology QR1-502 article 2023 ftdoajarticles https://doi.org/10.1128/spectrum.04094-22 2023-02-19T01:46:39Z ABSTRACT Small RNAs (sRNAs) play a very important role in gene regulation at the posttranscriptional level. However, sRNAs from nonmodel microorganisms, extremophiles in particular, have been rarely explored. We discovered a putative sRNA, termed Pf1 sRNA, in Pseudoalteromonas fuliginea BSW20308 isolated from the polar regions in our previous work. In this study, we identified the sRNA and investigated its regulatory role in gene expression under different temperatures. Pf1 sRNA was confirmed to be a new member of the CsrB family but has little sequence similarity with Escherichia coli CsrB. However, Pf1 sRNA was able to bind to CsrA from E. coli and P. fuliginea BSW20308 to regulate glycogen synthesis. The Pf1 sRNA knockout strain (ΔPf1) affected motility, fitness, and global gene expression in transcriptomes and proteomes at 4°C and 32°C. Genes related to carbon metabolism, amino acid metabolism, salinity tolerance, antibiotic resistance, oxidative stress, motility, chemotaxis, biofilm, and secretion systems were differentially expressed in the wild-type strain and the ΔPf1 mutant. Our study suggested that Pf1 sRNA might play an important role in response to environmental changes by regulating global gene expression. Specific targets of the Pf1 sRNA-CsrA system were tentatively proposed, such as genes involved in the type VI secretion system, TonB-dependent receptors, and response regulators, but most of them have an unknown function. Since this is the first study of CsrB family sRNA in Pseudoalteromonas and microbes from the polar regions, it provides a novel insight at the posttranscriptional level into the responses and adaptation to temperature changes in bacteria from extreme environments. This study also sheds light on the evolution of sRNA in extreme environments and expands the bacterial sRNA database. IMPORTANCE Previous research on microbial temperature adaptation has focused primarily on functional genes, with little attention paid to posttranscriptional regulation. Small RNAs, the major ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Microbiology Spectrum 11 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Pseudoalteromonas sRNA CsrA temperature regulation Microbiology QR1-502 |
spellingShingle |
Pseudoalteromonas sRNA CsrA temperature regulation Microbiology QR1-502 Jiao Wen Li Liao Zedong Duan Shiyuan Su Jin Zhang Bo Chen Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses |
topic_facet |
Pseudoalteromonas sRNA CsrA temperature regulation Microbiology QR1-502 |
description |
ABSTRACT Small RNAs (sRNAs) play a very important role in gene regulation at the posttranscriptional level. However, sRNAs from nonmodel microorganisms, extremophiles in particular, have been rarely explored. We discovered a putative sRNA, termed Pf1 sRNA, in Pseudoalteromonas fuliginea BSW20308 isolated from the polar regions in our previous work. In this study, we identified the sRNA and investigated its regulatory role in gene expression under different temperatures. Pf1 sRNA was confirmed to be a new member of the CsrB family but has little sequence similarity with Escherichia coli CsrB. However, Pf1 sRNA was able to bind to CsrA from E. coli and P. fuliginea BSW20308 to regulate glycogen synthesis. The Pf1 sRNA knockout strain (ΔPf1) affected motility, fitness, and global gene expression in transcriptomes and proteomes at 4°C and 32°C. Genes related to carbon metabolism, amino acid metabolism, salinity tolerance, antibiotic resistance, oxidative stress, motility, chemotaxis, biofilm, and secretion systems were differentially expressed in the wild-type strain and the ΔPf1 mutant. Our study suggested that Pf1 sRNA might play an important role in response to environmental changes by regulating global gene expression. Specific targets of the Pf1 sRNA-CsrA system were tentatively proposed, such as genes involved in the type VI secretion system, TonB-dependent receptors, and response regulators, but most of them have an unknown function. Since this is the first study of CsrB family sRNA in Pseudoalteromonas and microbes from the polar regions, it provides a novel insight at the posttranscriptional level into the responses and adaptation to temperature changes in bacteria from extreme environments. This study also sheds light on the evolution of sRNA in extreme environments and expands the bacterial sRNA database. IMPORTANCE Previous research on microbial temperature adaptation has focused primarily on functional genes, with little attention paid to posttranscriptional regulation. Small RNAs, the major ... |
format |
Article in Journal/Newspaper |
author |
Jiao Wen Li Liao Zedong Duan Shiyuan Su Jin Zhang Bo Chen |
author_facet |
Jiao Wen Li Liao Zedong Duan Shiyuan Su Jin Zhang Bo Chen |
author_sort |
Jiao Wen |
title |
Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses |
title_short |
Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses |
title_full |
Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses |
title_fullStr |
Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses |
title_full_unstemmed |
Identification and Regulatory Roles of a New Csr Small RNA from Arctic Pseudoalteromonas fuliginea BSW20308 in Temperature Responses |
title_sort |
identification and regulatory roles of a new csr small rna from arctic pseudoalteromonas fuliginea bsw20308 in temperature responses |
publisher |
American Society for Microbiology |
publishDate |
2023 |
url |
https://doi.org/10.1128/spectrum.04094-22 https://doaj.org/article/98e8825fe7ed44abb1b276705008f855 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Microbiology Spectrum, Vol 11, Iss 1 (2023) |
op_relation |
https://journals.asm.org/doi/10.1128/spectrum.04094-22 https://doaj.org/toc/2165-0497 doi:10.1128/spectrum.04094-22 2165-0497 https://doaj.org/article/98e8825fe7ed44abb1b276705008f855 |
op_doi |
https://doi.org/10.1128/spectrum.04094-22 |
container_title |
Microbiology Spectrum |
container_volume |
11 |
container_issue |
1 |
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1766344688386703360 |