Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica
Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms an...
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ftdoajarticles:oai:doaj.org/article:98c655526e7849cc8b4b00b59b41ff5e 2023-05-15T13:51:22+02:00 Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica Salihu Ibrahim Khadijah Nabilah Mohd Zahri Peter Convey Khalilah Abdul Khalil Claudio Gomez-Fuentes Azham Zulkarnain Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad 2020-11-01T00:00:00Z https://doi.org/10.1016/j.ejbt.2020.07.005 https://doaj.org/article/98c655526e7849cc8b4b00b59b41ff5e EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S0717345820300361 https://doaj.org/toc/0717-3458 0717-3458 doi:10.1016/j.ejbt.2020.07.005 https://doaj.org/article/98c655526e7849cc8b4b00b59b41ff5e Electronic Journal of Biotechnology, Vol 48, Iss , Pp 1-12 (2020) Antarctica Biodegradation Bioremediation Canola oil Cold-adapted Rhodococcus One-factor-at-a-time Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.1016/j.ejbt.2020.07.005 2022-12-31T02:54:20Z Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. Results: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. Conclusions: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica.How to cite: Ibrahim S, Zahri KNM, Convey P, et al. Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.07.005 Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Electronic Journal of Biotechnology 48 1 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica Biodegradation Bioremediation Canola oil Cold-adapted Rhodococcus One-factor-at-a-time Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 |
spellingShingle |
Antarctica Biodegradation Bioremediation Canola oil Cold-adapted Rhodococcus One-factor-at-a-time Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Salihu Ibrahim Khadijah Nabilah Mohd Zahri Peter Convey Khalilah Abdul Khalil Claudio Gomez-Fuentes Azham Zulkarnain Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
topic_facet |
Antarctica Biodegradation Bioremediation Canola oil Cold-adapted Rhodococcus One-factor-at-a-time Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 |
description |
Background: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. Results: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. Conclusions: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica.How to cite: Ibrahim S, Zahri KNM, Convey P, et al. Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.07.005 |
format |
Article in Journal/Newspaper |
author |
Salihu Ibrahim Khadijah Nabilah Mohd Zahri Peter Convey Khalilah Abdul Khalil Claudio Gomez-Fuentes Azham Zulkarnain Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
author_facet |
Salihu Ibrahim Khadijah Nabilah Mohd Zahri Peter Convey Khalilah Abdul Khalil Claudio Gomez-Fuentes Azham Zulkarnain Siti Aisyah Alias Gerardo González-Rocha Siti Aqlima Ahmad |
author_sort |
Salihu Ibrahim |
title |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_short |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_full |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_fullStr |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_full_unstemmed |
Optimisation of biodegradation conditions for waste canola oil by cold-adapted Rhodococcus sp. AQ5-07 from Antarctica |
title_sort |
optimisation of biodegradation conditions for waste canola oil by cold-adapted rhodococcus sp. aq5-07 from antarctica |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://doi.org/10.1016/j.ejbt.2020.07.005 https://doaj.org/article/98c655526e7849cc8b4b00b59b41ff5e |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Electronic Journal of Biotechnology, Vol 48, Iss , Pp 1-12 (2020) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0717345820300361 https://doaj.org/toc/0717-3458 0717-3458 doi:10.1016/j.ejbt.2020.07.005 https://doaj.org/article/98c655526e7849cc8b4b00b59b41ff5e |
op_doi |
https://doi.org/10.1016/j.ejbt.2020.07.005 |
container_title |
Electronic Journal of Biotechnology |
container_volume |
48 |
container_start_page |
1 |
op_container_end_page |
12 |
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1766255192409374720 |