Zika virus-like particle (VLP) based vaccine.
The newly emerged mosquito-borne Zika virus poses a major public challenge due to its ability to cause significant birth defects and neurological disorders. The impact of sexual transmission is unclear but raises further concerns about virus dissemination. No specific treatment or vaccine is current...
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ftdoajarticles:oai:doaj.org/article:98ad68310785481f8b9c36e702d8b3e7 2023-05-15T15:13:42+02:00 Zika virus-like particle (VLP) based vaccine. Hélène Boigard Alexandra Alimova George R Martin Al Katz Paul Gottlieb Jose M Galarza 2017-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005608 https://doaj.org/article/98ad68310785481f8b9c36e702d8b3e7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5436897?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005608 https://doaj.org/article/98ad68310785481f8b9c36e702d8b3e7 PLoS Neglected Tropical Diseases, Vol 11, Iss 5, p e0005608 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005608 2022-12-31T12:24:11Z The newly emerged mosquito-borne Zika virus poses a major public challenge due to its ability to cause significant birth defects and neurological disorders. The impact of sexual transmission is unclear but raises further concerns about virus dissemination. No specific treatment or vaccine is currently available, thus the development of a safe and effective vaccine is paramount. Here we describe a novel strategy to assemble Zika virus-like particles (VLPs) by co-expressing the structural (CprME) and non-structural (NS2B/NS3) proteins, and demonstrate their effectiveness as vaccines. VLPs are produced in a suspension culture of mammalian cells and self-assembled into particles closely resembling Zika viruses as shown by electron microscopy studies. We tested various VLP vaccines and compared them to analogous compositions of an inactivated Zika virus (In-ZIKV) used as a reference. VLP immunizations elicited high titers of antibodies, as did the In-ZIKV controls. However, in mice the VLP vaccine stimulated significantly higher virus neutralizing antibody titers than comparable formulations of the In-ZIKV vaccine. The serum neutralizing activity elicited by the VLP vaccine was enhanced using a higher VLP dose and with the addition of an adjuvant, reaching neutralizing titers greater than those detected in the serum of a patient who recovered from a Zika infection in Brazil in 2015. Discrepancies in neutralization levels between the VLP vaccine and the In-ZIKV suggest that chemical inactivation has deleterious effects on neutralizing epitopes within the E protein. This along with the inability of a VLP vaccine to cause infection makes it a preferable candidate for vaccine development. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 5 e0005608 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hélène Boigard Alexandra Alimova George R Martin Al Katz Paul Gottlieb Jose M Galarza Zika virus-like particle (VLP) based vaccine. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The newly emerged mosquito-borne Zika virus poses a major public challenge due to its ability to cause significant birth defects and neurological disorders. The impact of sexual transmission is unclear but raises further concerns about virus dissemination. No specific treatment or vaccine is currently available, thus the development of a safe and effective vaccine is paramount. Here we describe a novel strategy to assemble Zika virus-like particles (VLPs) by co-expressing the structural (CprME) and non-structural (NS2B/NS3) proteins, and demonstrate their effectiveness as vaccines. VLPs are produced in a suspension culture of mammalian cells and self-assembled into particles closely resembling Zika viruses as shown by electron microscopy studies. We tested various VLP vaccines and compared them to analogous compositions of an inactivated Zika virus (In-ZIKV) used as a reference. VLP immunizations elicited high titers of antibodies, as did the In-ZIKV controls. However, in mice the VLP vaccine stimulated significantly higher virus neutralizing antibody titers than comparable formulations of the In-ZIKV vaccine. The serum neutralizing activity elicited by the VLP vaccine was enhanced using a higher VLP dose and with the addition of an adjuvant, reaching neutralizing titers greater than those detected in the serum of a patient who recovered from a Zika infection in Brazil in 2015. Discrepancies in neutralization levels between the VLP vaccine and the In-ZIKV suggest that chemical inactivation has deleterious effects on neutralizing epitopes within the E protein. This along with the inability of a VLP vaccine to cause infection makes it a preferable candidate for vaccine development. |
format |
Article in Journal/Newspaper |
author |
Hélène Boigard Alexandra Alimova George R Martin Al Katz Paul Gottlieb Jose M Galarza |
author_facet |
Hélène Boigard Alexandra Alimova George R Martin Al Katz Paul Gottlieb Jose M Galarza |
author_sort |
Hélène Boigard |
title |
Zika virus-like particle (VLP) based vaccine. |
title_short |
Zika virus-like particle (VLP) based vaccine. |
title_full |
Zika virus-like particle (VLP) based vaccine. |
title_fullStr |
Zika virus-like particle (VLP) based vaccine. |
title_full_unstemmed |
Zika virus-like particle (VLP) based vaccine. |
title_sort |
zika virus-like particle (vlp) based vaccine. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005608 https://doaj.org/article/98ad68310785481f8b9c36e702d8b3e7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 5, p e0005608 (2017) |
op_relation |
http://europepmc.org/articles/PMC5436897?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005608 https://doaj.org/article/98ad68310785481f8b9c36e702d8b3e7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005608 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
5 |
container_start_page |
e0005608 |
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1766344221412818944 |