Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report.
Mucosal-associated invariant T (MAIT) cells are unconventional T lymphocytes with a semi-conserved TCRα, activated by the presentation of vitamin B metabolites by the MHC-I related protein, MR1, and with diverse innate and adaptive effector functions. The role of MAIT cells in acute intestinal infec...
Published in: | PLOS Neglected Tropical Diseases |
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2022
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ftdoajarticles:oai:doaj.org/article:98895e00d54f43b3bd070b2c5fc8c5e5 2023-05-15T15:13:53+02:00 Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. Taufiqur R Bhuiyan M Arifur Rahman Shubhanshi Trivedi Taliman Afroz Hasan Al Banna Mohammad Rubel Hoq Ioana Pop Owen Jensen Rasheduzzaman Rashu Muhammad Ikhtear Uddin Motaher Hossain Ashraful I Khan Fahima Chowdhury Jason B Harris Stephen B Calderwood Edward T Ryan Firdausi Qadri Daniel T Leung 2022-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010411 https://doaj.org/article/98895e00d54f43b3bd070b2c5fc8c5e5 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010411 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010411 https://doaj.org/article/98895e00d54f43b3bd070b2c5fc8c5e5 PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010411 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010411 2023-01-15T01:24:08Z Mucosal-associated invariant T (MAIT) cells are unconventional T lymphocytes with a semi-conserved TCRα, activated by the presentation of vitamin B metabolites by the MHC-I related protein, MR1, and with diverse innate and adaptive effector functions. The role of MAIT cells in acute intestinal infections, especially at the mucosal level, is not well known. Here, we analyzed the presence and phenotype of MAIT cells in duodenal biopsies and paired peripheral blood samples, in patients during and after culture-confirmed Vibrio cholerae O1 infection. Immunohistochemical staining of duodenal biopsies from cholera patients (n = 5, median age 32 years, range 26-44, 1 female) identified MAIT cells in the lamina propria of the crypts, but not the villi. By flow cytometry (n = 10, median age 31 years, range 23-36, 1 female), we showed that duodenal MAIT cells are more activated than peripheral MAIT cells (p < 0.01 across time points), although there were no significant differences between duodenal MAIT cells at day 2 and day 30. We found fecal markers of intestinal permeability and inflammation to be correlated with the loss of duodenal (but not peripheral) MAIT cells, and single-cell sequencing revealed differing T cell receptor usage between the duodenal and peripheral blood MAIT cells. In this preliminary report limited by a small sample size, we show that MAIT cells are present in the lamina propria of the duodenum during V. cholerae infection, and more activated than those in the blood. Future work into the trafficking and tissue-resident function of MAIT cells is warranted. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 5 e0010411 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Taufiqur R Bhuiyan M Arifur Rahman Shubhanshi Trivedi Taliman Afroz Hasan Al Banna Mohammad Rubel Hoq Ioana Pop Owen Jensen Rasheduzzaman Rashu Muhammad Ikhtear Uddin Motaher Hossain Ashraful I Khan Fahima Chowdhury Jason B Harris Stephen B Calderwood Edward T Ryan Firdausi Qadri Daniel T Leung Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mucosal-associated invariant T (MAIT) cells are unconventional T lymphocytes with a semi-conserved TCRα, activated by the presentation of vitamin B metabolites by the MHC-I related protein, MR1, and with diverse innate and adaptive effector functions. The role of MAIT cells in acute intestinal infections, especially at the mucosal level, is not well known. Here, we analyzed the presence and phenotype of MAIT cells in duodenal biopsies and paired peripheral blood samples, in patients during and after culture-confirmed Vibrio cholerae O1 infection. Immunohistochemical staining of duodenal biopsies from cholera patients (n = 5, median age 32 years, range 26-44, 1 female) identified MAIT cells in the lamina propria of the crypts, but not the villi. By flow cytometry (n = 10, median age 31 years, range 23-36, 1 female), we showed that duodenal MAIT cells are more activated than peripheral MAIT cells (p < 0.01 across time points), although there were no significant differences between duodenal MAIT cells at day 2 and day 30. We found fecal markers of intestinal permeability and inflammation to be correlated with the loss of duodenal (but not peripheral) MAIT cells, and single-cell sequencing revealed differing T cell receptor usage between the duodenal and peripheral blood MAIT cells. In this preliminary report limited by a small sample size, we show that MAIT cells are present in the lamina propria of the duodenum during V. cholerae infection, and more activated than those in the blood. Future work into the trafficking and tissue-resident function of MAIT cells is warranted. |
format |
Article in Journal/Newspaper |
author |
Taufiqur R Bhuiyan M Arifur Rahman Shubhanshi Trivedi Taliman Afroz Hasan Al Banna Mohammad Rubel Hoq Ioana Pop Owen Jensen Rasheduzzaman Rashu Muhammad Ikhtear Uddin Motaher Hossain Ashraful I Khan Fahima Chowdhury Jason B Harris Stephen B Calderwood Edward T Ryan Firdausi Qadri Daniel T Leung |
author_facet |
Taufiqur R Bhuiyan M Arifur Rahman Shubhanshi Trivedi Taliman Afroz Hasan Al Banna Mohammad Rubel Hoq Ioana Pop Owen Jensen Rasheduzzaman Rashu Muhammad Ikhtear Uddin Motaher Hossain Ashraful I Khan Fahima Chowdhury Jason B Harris Stephen B Calderwood Edward T Ryan Firdausi Qadri Daniel T Leung |
author_sort |
Taufiqur R Bhuiyan |
title |
Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. |
title_short |
Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. |
title_full |
Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. |
title_fullStr |
Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. |
title_full_unstemmed |
Mucosal-Associated Invariant T (MAIT) cells are highly activated in duodenal tissue of humans with Vibrio cholerae O1 infection: A preliminary report. |
title_sort |
mucosal-associated invariant t (mait) cells are highly activated in duodenal tissue of humans with vibrio cholerae o1 infection: a preliminary report. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010411 https://doaj.org/article/98895e00d54f43b3bd070b2c5fc8c5e5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010411 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010411 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010411 https://doaj.org/article/98895e00d54f43b3bd070b2c5fc8c5e5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010411 |
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PLOS Neglected Tropical Diseases |
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16 |
container_issue |
5 |
container_start_page |
e0010411 |
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