Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas
Abstract The Pacific oyster, Crassostrea gigas, is a traditional food worldwide. The soft body of the oyster can easily accumulate heavy metals such as cadmium (Cd). To clarify the molecular mechanism of Cd accumulation in the viscera of C. gigas, we identified Cd-binding proteins. 5,10,15,20-Tetrap...
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ftdoajarticles:oai:doaj.org/article:983c6b04f0784e28b3e6a1135551ce95 2023-05-15T15:58:12+02:00 Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas Zehua Zheng Kazuhiro Kawakami Dingkun Zhang Lumi Negishi Mohamed Abomosallam Tomiko Asakura Koji Nagata Michio Suzuki 2021-05-01T00:00:00Z https://doi.org/10.1038/s41598-021-90882-4 https://doaj.org/article/983c6b04f0784e28b3e6a1135551ce95 EN eng Nature Portfolio https://doi.org/10.1038/s41598-021-90882-4 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-021-90882-4 2045-2322 https://doaj.org/article/983c6b04f0784e28b3e6a1135551ce95 Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1038/s41598-021-90882-4 2022-12-31T11:18:38Z Abstract The Pacific oyster, Crassostrea gigas, is a traditional food worldwide. The soft body of the oyster can easily accumulate heavy metals such as cadmium (Cd). To clarify the molecular mechanism of Cd accumulation in the viscera of C. gigas, we identified Cd-binding proteins. 5,10,15,20-Tetraphenyl-21H,23H-porphinetetrasulfonic acid, disulfuric acid, tetrahydrate, and Cd-binding competition experiments using immobilized metal ion affinity chromatography revealed the binding of water-soluble high molecular weight proteins to Cd, including C. gigas protein disulfide isomerase (cgPDI). Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analyses revealed two CGHC motifs in cgPDI. The binding between Cd and rcgPDI was confirmed through a Cd-binding experiment using the TPPS method. Isothermal titration calorimetry (ITC) revealed the binding of two Cd ions to one molecule of rcgPDI. Circular dichroism (CD) spectrum and tryptophan fluorescence analyses demonstrated that the rcgPDI bound to Cd. The binding markedly changed the two-dimensional or three-dimensional structures. The activity of rcgPDI measured by a PDI Activity Assay Kit was more affected by the addition of Cd than by human PDI. Immunological analyses indicated that C. gigas contained cgPDI at a concentration of 1.0 nmol/g (viscera wet weight). The combination of ITC and quantification results revealed that Cd-binding to cgPDI accounted for 20% of the total bound Cd in the visceral mass. The findings provide new insights into the defense mechanisms of invertebrates against Cd. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Scientific Reports 11 1 |
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Medicine R Science Q Zehua Zheng Kazuhiro Kawakami Dingkun Zhang Lumi Negishi Mohamed Abomosallam Tomiko Asakura Koji Nagata Michio Suzuki Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas |
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Medicine R Science Q |
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Abstract The Pacific oyster, Crassostrea gigas, is a traditional food worldwide. The soft body of the oyster can easily accumulate heavy metals such as cadmium (Cd). To clarify the molecular mechanism of Cd accumulation in the viscera of C. gigas, we identified Cd-binding proteins. 5,10,15,20-Tetraphenyl-21H,23H-porphinetetrasulfonic acid, disulfuric acid, tetrahydrate, and Cd-binding competition experiments using immobilized metal ion affinity chromatography revealed the binding of water-soluble high molecular weight proteins to Cd, including C. gigas protein disulfide isomerase (cgPDI). Liquid chromatography–tandem mass spectrometry (LC–MS/MS) analyses revealed two CGHC motifs in cgPDI. The binding between Cd and rcgPDI was confirmed through a Cd-binding experiment using the TPPS method. Isothermal titration calorimetry (ITC) revealed the binding of two Cd ions to one molecule of rcgPDI. Circular dichroism (CD) spectrum and tryptophan fluorescence analyses demonstrated that the rcgPDI bound to Cd. The binding markedly changed the two-dimensional or three-dimensional structures. The activity of rcgPDI measured by a PDI Activity Assay Kit was more affected by the addition of Cd than by human PDI. Immunological analyses indicated that C. gigas contained cgPDI at a concentration of 1.0 nmol/g (viscera wet weight). The combination of ITC and quantification results revealed that Cd-binding to cgPDI accounted for 20% of the total bound Cd in the visceral mass. The findings provide new insights into the defense mechanisms of invertebrates against Cd. |
format |
Article in Journal/Newspaper |
author |
Zehua Zheng Kazuhiro Kawakami Dingkun Zhang Lumi Negishi Mohamed Abomosallam Tomiko Asakura Koji Nagata Michio Suzuki |
author_facet |
Zehua Zheng Kazuhiro Kawakami Dingkun Zhang Lumi Negishi Mohamed Abomosallam Tomiko Asakura Koji Nagata Michio Suzuki |
author_sort |
Zehua Zheng |
title |
Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas |
title_short |
Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas |
title_full |
Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas |
title_fullStr |
Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas |
title_full_unstemmed |
Identification and functional analysis of cadmium-binding protein in the visceral mass of Crassostrea gigas |
title_sort |
identification and functional analysis of cadmium-binding protein in the visceral mass of crassostrea gigas |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doi.org/10.1038/s41598-021-90882-4 https://doaj.org/article/983c6b04f0784e28b3e6a1135551ce95 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
op_relation |
https://doi.org/10.1038/s41598-021-90882-4 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-021-90882-4 2045-2322 https://doaj.org/article/983c6b04f0784e28b3e6a1135551ce95 |
op_doi |
https://doi.org/10.1038/s41598-021-90882-4 |
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Scientific Reports |
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11 |
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1 |
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1766393931481743360 |