Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research

Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and...

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Published in:Metabolites
Main Authors: Lada Ivanova, Oscar D. Rangel-Huerta, Haitham Tartor, Mona C. Gjessing, Maria K. Dahle, Silvio Uhlig
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2021
Subjects:
Online Access:https://doi.org/10.3390/metabo12010028
https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01
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spelling ftdoajarticles:oai:doaj.org/article:9836c16da7cc4f7eae3808aef127bd01 2023-05-15T15:31:18+02:00 Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig 2021-12-01T00:00:00Z https://doi.org/10.3390/metabo12010028 https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01 EN eng MDPI AG https://www.mdpi.com/2218-1989/12/1/28 https://doaj.org/toc/2218-1989 doi:10.3390/metabo12010028 2218-1989 https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01 Metabolites, Vol 12, Iss 28, p 28 (2021) gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3390/metabo12010028 2022-12-30T22:03:12Z Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon ( Salmo salar ) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Metabolites 12 1 28
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic gill mucus
skin mucus
Atlantic salmon
biomarkers
non-invasive sampling
data normalization
Microbiology
QR1-502
spellingShingle gill mucus
skin mucus
Atlantic salmon
biomarkers
non-invasive sampling
data normalization
Microbiology
QR1-502
Lada Ivanova
Oscar D. Rangel-Huerta
Haitham Tartor
Mona C. Gjessing
Maria K. Dahle
Silvio Uhlig
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
topic_facet gill mucus
skin mucus
Atlantic salmon
biomarkers
non-invasive sampling
data normalization
Microbiology
QR1-502
description Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon ( Salmo salar ) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research.
format Article in Journal/Newspaper
author Lada Ivanova
Oscar D. Rangel-Huerta
Haitham Tartor
Mona C. Gjessing
Maria K. Dahle
Silvio Uhlig
author_facet Lada Ivanova
Oscar D. Rangel-Huerta
Haitham Tartor
Mona C. Gjessing
Maria K. Dahle
Silvio Uhlig
author_sort Lada Ivanova
title Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_short Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_full Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_fullStr Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_full_unstemmed Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
title_sort fish skin and gill mucus: a source of metabolites for non-invasive health monitoring and research
publisher MDPI AG
publishDate 2021
url https://doi.org/10.3390/metabo12010028
https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source Metabolites, Vol 12, Iss 28, p 28 (2021)
op_relation https://www.mdpi.com/2218-1989/12/1/28
https://doaj.org/toc/2218-1989
doi:10.3390/metabo12010028
2218-1989
https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01
op_doi https://doi.org/10.3390/metabo12010028
container_title Metabolites
container_volume 12
container_issue 1
container_start_page 28
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