Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and...
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ftdoajarticles:oai:doaj.org/article:9836c16da7cc4f7eae3808aef127bd01 2023-05-15T15:31:18+02:00 Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig 2021-12-01T00:00:00Z https://doi.org/10.3390/metabo12010028 https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01 EN eng MDPI AG https://www.mdpi.com/2218-1989/12/1/28 https://doaj.org/toc/2218-1989 doi:10.3390/metabo12010028 2218-1989 https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01 Metabolites, Vol 12, Iss 28, p 28 (2021) gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3390/metabo12010028 2022-12-30T22:03:12Z Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon ( Salmo salar ) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Metabolites 12 1 28 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Microbiology QR1-502 |
spellingShingle |
gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Microbiology QR1-502 Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
topic_facet |
gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Microbiology QR1-502 |
description |
Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon ( Salmo salar ) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research. |
format |
Article in Journal/Newspaper |
author |
Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig |
author_facet |
Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig |
author_sort |
Lada Ivanova |
title |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_short |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_full |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_fullStr |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_full_unstemmed |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_sort |
fish skin and gill mucus: a source of metabolites for non-invasive health monitoring and research |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/metabo12010028 https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Metabolites, Vol 12, Iss 28, p 28 (2021) |
op_relation |
https://www.mdpi.com/2218-1989/12/1/28 https://doaj.org/toc/2218-1989 doi:10.3390/metabo12010028 2218-1989 https://doaj.org/article/9836c16da7cc4f7eae3808aef127bd01 |
op_doi |
https://doi.org/10.3390/metabo12010028 |
container_title |
Metabolites |
container_volume |
12 |
container_issue |
1 |
container_start_page |
28 |
_version_ |
1766361795535044608 |