DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL
Dengue virus (DENV) is the most frequent arbovirus worldwide. In this study, we report a large outbreak in Mato Grosso State (MT). Serum samples from 604 patients with acute febrile illness for less than five days were inoculated in C6/36 cells, then infected cells were subjected to an indirect immu...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:97f125b8dd044975a90642009c5b892b 2024-09-09T19:27:03+00:00 DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL Letícia Borges da Silva HEINEN Nayara ZUCHI Belgath Fernandes CARDOSO Marcelo Adriano Mendes dos SANTOS Mauricio Lacerda NOGUEIRA Renata DEZENGRINI-SLHESSARENKO 2015-12-01T00:00:00Z https://doi.org/10.1590/S0036-46652015000600005 https://doaj.org/article/97f125b8dd044975a90642009c5b892b EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000600489&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652015000600005 https://doaj.org/article/97f125b8dd044975a90642009c5b892b Revista do Instituto de Medicina Tropical de São Paulo, Vol 57, Iss 6, Pp 489-496 (2015) Arbovirus DENV Virological surveillance Epidemiology Molecular detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2015 ftdoajarticles https://doi.org/10.1590/S0036-46652015000600005 2024-08-05T17:49:30Z Dengue virus (DENV) is the most frequent arbovirus worldwide. In this study, we report a large outbreak in Mato Grosso State (MT). Serum samples from 604 patients with acute febrile illness for less than five days were inoculated in C6/36 cells, then infected cells were subjected to an indirect immunofluorescence test for DENV serotypes and yellow fever virus. Serum samples were submitted to a multiplex-semi-nested-RT-PCR for 11 flaviviruses. DENV-4 was isolated in 150/604 (24.8%) and DENV-1 in 19/604 (3.1%) specimens. By RT-PCR, 331 (54.8%) samples tested positive for DENV; 321 had single infections (DENV-4 n = 305; DENV-1 n = 15; DENV-3 n = 1), nine had co-infections of DENV-1/DENV-4, and one of DENV-2/DENV-4. DENV-4 was detected in 315/331 (95.2%) positive patients from 17 municipalities, and DENV-1 in 24/331 (7.2%) patients from five cities in north-central MT and the city of Cuiaba. The incidence of infection was higher in patients aged 20-39 (142/331; 42.9%). The NS5 partial nucleotide sequence of DENV-1 was most similar to that of genotype V, DENV-2 to Southeast Asian/American, DENV-3 to genotype III, and DENV-4 to genotype II strains, considered the most frequent strains in Brazil. This outbreak coincided with the introduction of DENV-4 in the state. Cuiaba was hyperendemic for the four DENV serotypes, highlighting the necessity for arbovirus surveillance in MT. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 57 6 489 496 |
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ftdoajarticles |
language |
English |
topic |
Arbovirus DENV Virological surveillance Epidemiology Molecular detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arbovirus DENV Virological surveillance Epidemiology Molecular detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Letícia Borges da Silva HEINEN Nayara ZUCHI Belgath Fernandes CARDOSO Marcelo Adriano Mendes dos SANTOS Mauricio Lacerda NOGUEIRA Renata DEZENGRINI-SLHESSARENKO DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL |
topic_facet |
Arbovirus DENV Virological surveillance Epidemiology Molecular detection Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Dengue virus (DENV) is the most frequent arbovirus worldwide. In this study, we report a large outbreak in Mato Grosso State (MT). Serum samples from 604 patients with acute febrile illness for less than five days were inoculated in C6/36 cells, then infected cells were subjected to an indirect immunofluorescence test for DENV serotypes and yellow fever virus. Serum samples were submitted to a multiplex-semi-nested-RT-PCR for 11 flaviviruses. DENV-4 was isolated in 150/604 (24.8%) and DENV-1 in 19/604 (3.1%) specimens. By RT-PCR, 331 (54.8%) samples tested positive for DENV; 321 had single infections (DENV-4 n = 305; DENV-1 n = 15; DENV-3 n = 1), nine had co-infections of DENV-1/DENV-4, and one of DENV-2/DENV-4. DENV-4 was detected in 315/331 (95.2%) positive patients from 17 municipalities, and DENV-1 in 24/331 (7.2%) patients from five cities in north-central MT and the city of Cuiaba. The incidence of infection was higher in patients aged 20-39 (142/331; 42.9%). The NS5 partial nucleotide sequence of DENV-1 was most similar to that of genotype V, DENV-2 to Southeast Asian/American, DENV-3 to genotype III, and DENV-4 to genotype II strains, considered the most frequent strains in Brazil. This outbreak coincided with the introduction of DENV-4 in the state. Cuiaba was hyperendemic for the four DENV serotypes, highlighting the necessity for arbovirus surveillance in MT. |
format |
Article in Journal/Newspaper |
author |
Letícia Borges da Silva HEINEN Nayara ZUCHI Belgath Fernandes CARDOSO Marcelo Adriano Mendes dos SANTOS Mauricio Lacerda NOGUEIRA Renata DEZENGRINI-SLHESSARENKO |
author_facet |
Letícia Borges da Silva HEINEN Nayara ZUCHI Belgath Fernandes CARDOSO Marcelo Adriano Mendes dos SANTOS Mauricio Lacerda NOGUEIRA Renata DEZENGRINI-SLHESSARENKO |
author_sort |
Letícia Borges da Silva HEINEN |
title |
DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL |
title_short |
DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL |
title_full |
DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL |
title_fullStr |
DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL |
title_full_unstemmed |
DENGUE OUTBREAK IN MATO GROSSO STATE, MIDWESTERN BRAZIL |
title_sort |
dengue outbreak in mato grosso state, midwestern brazil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2015 |
url |
https://doi.org/10.1590/S0036-46652015000600005 https://doaj.org/article/97f125b8dd044975a90642009c5b892b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 57, Iss 6, Pp 489-496 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000600489&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652015000600005 https://doaj.org/article/97f125b8dd044975a90642009c5b892b |
op_doi |
https://doi.org/10.1590/S0036-46652015000600005 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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57 |
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489 |
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