The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi.
The pathway for RNA interference is widespread in metazoans and participates in numerous cellular tasks, from gene silencing to chromatin remodeling and protection against retrotransposition. The unicellular eukaryote Trypanosoma cruzi is missing the canonical RNAi pathway and is unable to induce RN...
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Online Access: | https://doi.org/10.1371/journal.pntd.0001283 https://doaj.org/article/97eee3fce9e943188af8da3a2191fa2e |
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ftdoajarticles:oai:doaj.org/article:97eee3fce9e943188af8da3a2191fa2e 2023-05-15T15:05:01+02:00 The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. Oscar Franzén Erik Arner Marcela Ferella Daniel Nilsson Patricia Respuela Piero Carninci Yoshihide Hayashizaki Lena Aslund Björn Andersson Carsten O Daub 2011-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001283 https://doaj.org/article/97eee3fce9e943188af8da3a2191fa2e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3166047?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001283 https://doaj.org/article/97eee3fce9e943188af8da3a2191fa2e PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1283 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001283 2022-12-31T08:13:09Z The pathway for RNA interference is widespread in metazoans and participates in numerous cellular tasks, from gene silencing to chromatin remodeling and protection against retrotransposition. The unicellular eukaryote Trypanosoma cruzi is missing the canonical RNAi pathway and is unable to induce RNAi-related processes. To further understand alternative RNA pathways operating in this organism, we have performed deep sequencing and genome-wide analyses of a size-fractioned cDNA library (16-61 nt) from the epimastigote life stage. Deep sequencing generated 582,243 short sequences of which 91% could be aligned with the genome sequence. About 95-98% of the aligned data (depending on the haplotype) corresponded to small RNAs derived from tRNAs, rRNAs, snRNAs and snoRNAs. The largest class consisted of tRNA-derived small RNAs which primarily originated from the 3' end of tRNAs, followed by small RNAs derived from rRNA. The remaining sequences revealed the presence of 92 novel transcribed loci, of which 79 did not show homology to known RNA classes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 8 e1283 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Oscar Franzén Erik Arner Marcela Ferella Daniel Nilsson Patricia Respuela Piero Carninci Yoshihide Hayashizaki Lena Aslund Björn Andersson Carsten O Daub The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The pathway for RNA interference is widespread in metazoans and participates in numerous cellular tasks, from gene silencing to chromatin remodeling and protection against retrotransposition. The unicellular eukaryote Trypanosoma cruzi is missing the canonical RNAi pathway and is unable to induce RNAi-related processes. To further understand alternative RNA pathways operating in this organism, we have performed deep sequencing and genome-wide analyses of a size-fractioned cDNA library (16-61 nt) from the epimastigote life stage. Deep sequencing generated 582,243 short sequences of which 91% could be aligned with the genome sequence. About 95-98% of the aligned data (depending on the haplotype) corresponded to small RNAs derived from tRNAs, rRNAs, snRNAs and snoRNAs. The largest class consisted of tRNA-derived small RNAs which primarily originated from the 3' end of tRNAs, followed by small RNAs derived from rRNA. The remaining sequences revealed the presence of 92 novel transcribed loci, of which 79 did not show homology to known RNA classes. |
format |
Article in Journal/Newspaper |
author |
Oscar Franzén Erik Arner Marcela Ferella Daniel Nilsson Patricia Respuela Piero Carninci Yoshihide Hayashizaki Lena Aslund Björn Andersson Carsten O Daub |
author_facet |
Oscar Franzén Erik Arner Marcela Ferella Daniel Nilsson Patricia Respuela Piero Carninci Yoshihide Hayashizaki Lena Aslund Björn Andersson Carsten O Daub |
author_sort |
Oscar Franzén |
title |
The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. |
title_short |
The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. |
title_full |
The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. |
title_fullStr |
The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. |
title_full_unstemmed |
The short non-coding transcriptome of the protozoan parasite Trypanosoma cruzi. |
title_sort |
short non-coding transcriptome of the protozoan parasite trypanosoma cruzi. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001283 https://doaj.org/article/97eee3fce9e943188af8da3a2191fa2e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 8, p e1283 (2011) |
op_relation |
http://europepmc.org/articles/PMC3166047?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001283 https://doaj.org/article/97eee3fce9e943188af8da3a2191fa2e |
op_doi |
https://doi.org/10.1371/journal.pntd.0001283 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
8 |
container_start_page |
e1283 |
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1766336791025025024 |