Azathioprine favourably influences the course of malaria
Abstract Background Azathioprine triggers suicidal erythrocyte death or eryptosis, characterized by cell shrinkage and exposure of phosphatidylserine at the erythrocyte surface. Eryptosis may accelerate the clearance of Plasmodium -infected erythrocytes. The present study thus explored whether azath...
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ftdoajarticles:oai:doaj.org/article:97bfc50879a0443db0b3cef86e8a8002 2023-05-15T15:08:48+02:00 Azathioprine favourably influences the course of malaria Huber Stephan M Föller Michael Geiger Corinna Koka Saisudha Bobbala Diwakar Lang Florian 2009-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-102 https://doaj.org/article/97bfc50879a0443db0b3cef86e8a8002 EN eng BMC http://www.malariajournal.com/content/8/1/102 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-102 1475-2875 https://doaj.org/article/97bfc50879a0443db0b3cef86e8a8002 Malaria Journal, Vol 8, Iss 1, p 102 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-102 2022-12-30T22:26:21Z Abstract Background Azathioprine triggers suicidal erythrocyte death or eryptosis, characterized by cell shrinkage and exposure of phosphatidylserine at the erythrocyte surface. Eryptosis may accelerate the clearance of Plasmodium -infected erythrocytes. The present study thus explored whether azathioprine influences eryptosis of Plasmodium -infected erythrocytes, development of parasitaemia and thus the course of malaria. Methods Human erythrocytes were infected in vitro with Plasmodium falciparum (P. falciparum) (strain BinH) in the absence and presence of azathioprine (0.001 – 10 μM), parasitaemia determined utilizing Syto16, phosphatidylserine exposure estimated from annexin V-binding and cell volume from forward scatter in FACS analysis. Mice were infected with Plasmodium berghei (P. berghei) ANKA by injecting parasitized murine erythrocytes (1 × 10 6 ) intraperitoneally. Where indicated azathioprine (5 mg/kg b.w.) was administered subcutaneously from the eighth day of infection. Results In vitro infection of human erythrocytes with P. falciparum increased annexin V-binding and initially decreased forward scatter, effects significantly augmented by azathioprine. At higher concentrations azathioprine significantly decreased intraerythrocytic DNA/RNA content (≥ 1 μM) and in vitro parasitaemia (≥ 1 μM). Administration of azathioprine significantly decreased the parasitaemia of circulating erythrocytes and increased the survival of P. berghei -infected mice (from 0% to 77% 22 days after infection). Conclusion Azathioprine inhibits intraerythrocytic growth of P. falciparum , enhances suicidal death of infected erythrocytes, decreases parasitaemia and fosters host survival during malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 102 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Huber Stephan M Föller Michael Geiger Corinna Koka Saisudha Bobbala Diwakar Lang Florian Azathioprine favourably influences the course of malaria |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Azathioprine triggers suicidal erythrocyte death or eryptosis, characterized by cell shrinkage and exposure of phosphatidylserine at the erythrocyte surface. Eryptosis may accelerate the clearance of Plasmodium -infected erythrocytes. The present study thus explored whether azathioprine influences eryptosis of Plasmodium -infected erythrocytes, development of parasitaemia and thus the course of malaria. Methods Human erythrocytes were infected in vitro with Plasmodium falciparum (P. falciparum) (strain BinH) in the absence and presence of azathioprine (0.001 – 10 μM), parasitaemia determined utilizing Syto16, phosphatidylserine exposure estimated from annexin V-binding and cell volume from forward scatter in FACS analysis. Mice were infected with Plasmodium berghei (P. berghei) ANKA by injecting parasitized murine erythrocytes (1 × 10 6 ) intraperitoneally. Where indicated azathioprine (5 mg/kg b.w.) was administered subcutaneously from the eighth day of infection. Results In vitro infection of human erythrocytes with P. falciparum increased annexin V-binding and initially decreased forward scatter, effects significantly augmented by azathioprine. At higher concentrations azathioprine significantly decreased intraerythrocytic DNA/RNA content (≥ 1 μM) and in vitro parasitaemia (≥ 1 μM). Administration of azathioprine significantly decreased the parasitaemia of circulating erythrocytes and increased the survival of P. berghei -infected mice (from 0% to 77% 22 days after infection). Conclusion Azathioprine inhibits intraerythrocytic growth of P. falciparum , enhances suicidal death of infected erythrocytes, decreases parasitaemia and fosters host survival during malaria. |
format |
Article in Journal/Newspaper |
author |
Huber Stephan M Föller Michael Geiger Corinna Koka Saisudha Bobbala Diwakar Lang Florian |
author_facet |
Huber Stephan M Föller Michael Geiger Corinna Koka Saisudha Bobbala Diwakar Lang Florian |
author_sort |
Huber Stephan M |
title |
Azathioprine favourably influences the course of malaria |
title_short |
Azathioprine favourably influences the course of malaria |
title_full |
Azathioprine favourably influences the course of malaria |
title_fullStr |
Azathioprine favourably influences the course of malaria |
title_full_unstemmed |
Azathioprine favourably influences the course of malaria |
title_sort |
azathioprine favourably influences the course of malaria |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-102 https://doaj.org/article/97bfc50879a0443db0b3cef86e8a8002 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 102 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/102 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-102 1475-2875 https://doaj.org/article/97bfc50879a0443db0b3cef86e8a8002 |
op_doi |
https://doi.org/10.1186/1475-2875-8-102 |
container_title |
Malaria Journal |
container_volume |
8 |
container_issue |
1 |
container_start_page |
102 |
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1766340092067053568 |