Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus.
Protein metabolism, including the interrelated processes of synthesis and degradation, mediates the growth of an animal. In ectothermic animals, protein metabolism is responsive to changes in both biotic and abiotic conditions. This study aimed to characterise responses of protein metabolism to food...
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ftdoajarticles:oai:doaj.org/article:9781400027f148d39e900ef47cae4338 2023-05-15T14:29:59+02:00 Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. Alicia A Cassidy Roxanne J Saulnier Simon G Lamarre 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0153364 https://doaj.org/article/9781400027f148d39e900ef47cae4338 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4838323?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0153364 https://doaj.org/article/9781400027f148d39e900ef47cae4338 PLoS ONE, Vol 11, Iss 4, p e0153364 (2016) Medicine R Science Q article 2016 ftdoajarticles https://doi.org/10.1371/journal.pone.0153364 2022-12-31T01:50:54Z Protein metabolism, including the interrelated processes of synthesis and degradation, mediates the growth of an animal. In ectothermic animals, protein metabolism is responsive to changes in both biotic and abiotic conditions. This study aimed to characterise responses of protein metabolism to food deprivation that occur in the coldwater salmonid, Arctic charr, Salvelinus alpinus. We compared two groups of Arctic charr: one fed continuously and the other deprived of food for 36 days. We measured the fractional rate of protein synthesis (KS) in individuals from the fed and fasted groups using a flooding dose technique modified for the use of deuterium-labelled phenylalanine. The enzyme activities of the three major protein degradation pathways (ubiquitin proteasome, lysosomal cathepsins and the calpain systems) were measured in the same fish. This study is the first to measure both KS and the enzymatic activity of protein degradation in the same fish, allowing us to examine the apparent contribution of different protein degradation pathways to protein turnover in various tissues (red and white muscle, liver, heart and gills). KS was lower in the white muscle and in liver of the fasted fish compared to the fed fish. There were no observable effects of food deprivation on the protease activities in any of the tissues with the exception of liver, where the ubiquitin proteasome pathway seemed to be activated during fasting conditions. Lysosomal proteolysis appears to be the primary degradation pathway for muscle protein, while the ubiquitin proteasome pathway seems to predominate in the liver. We speculate that Arctic charr regulate protein metabolism during food deprivation to conserve proteins. Article in Journal/Newspaper Arctic charr Arctic Salvelinus alpinus Directory of Open Access Journals: DOAJ Articles Arctic PLOS ONE 11 4 e0153364 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Alicia A Cassidy Roxanne J Saulnier Simon G Lamarre Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. |
topic_facet |
Medicine R Science Q |
description |
Protein metabolism, including the interrelated processes of synthesis and degradation, mediates the growth of an animal. In ectothermic animals, protein metabolism is responsive to changes in both biotic and abiotic conditions. This study aimed to characterise responses of protein metabolism to food deprivation that occur in the coldwater salmonid, Arctic charr, Salvelinus alpinus. We compared two groups of Arctic charr: one fed continuously and the other deprived of food for 36 days. We measured the fractional rate of protein synthesis (KS) in individuals from the fed and fasted groups using a flooding dose technique modified for the use of deuterium-labelled phenylalanine. The enzyme activities of the three major protein degradation pathways (ubiquitin proteasome, lysosomal cathepsins and the calpain systems) were measured in the same fish. This study is the first to measure both KS and the enzymatic activity of protein degradation in the same fish, allowing us to examine the apparent contribution of different protein degradation pathways to protein turnover in various tissues (red and white muscle, liver, heart and gills). KS was lower in the white muscle and in liver of the fasted fish compared to the fed fish. There were no observable effects of food deprivation on the protease activities in any of the tissues with the exception of liver, where the ubiquitin proteasome pathway seemed to be activated during fasting conditions. Lysosomal proteolysis appears to be the primary degradation pathway for muscle protein, while the ubiquitin proteasome pathway seems to predominate in the liver. We speculate that Arctic charr regulate protein metabolism during food deprivation to conserve proteins. |
format |
Article in Journal/Newspaper |
author |
Alicia A Cassidy Roxanne J Saulnier Simon G Lamarre |
author_facet |
Alicia A Cassidy Roxanne J Saulnier Simon G Lamarre |
author_sort |
Alicia A Cassidy |
title |
Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. |
title_short |
Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. |
title_full |
Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. |
title_fullStr |
Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. |
title_full_unstemmed |
Adjustments of Protein Metabolism in Fasting Arctic Charr, Salvelinus alpinus. |
title_sort |
adjustments of protein metabolism in fasting arctic charr, salvelinus alpinus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0153364 https://doaj.org/article/9781400027f148d39e900ef47cae4338 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_source |
PLoS ONE, Vol 11, Iss 4, p e0153364 (2016) |
op_relation |
http://europepmc.org/articles/PMC4838323?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0153364 https://doaj.org/article/9781400027f148d39e900ef47cae4338 |
op_doi |
https://doi.org/10.1371/journal.pone.0153364 |
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PLOS ONE |
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11 |
container_issue |
4 |
container_start_page |
e0153364 |
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