Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem
Considering the importance of Caspian Sea sturgeon conservation according to CITES rules, finding effective and efficient methods for tracing and identifying sturgeon species are necessary. Residual DNA detection in fish ponds can be used as a model for tracing fish environmental DNA (eDNA) in river...
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University of Mazandaran
2023
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Online Access: | https://doi.org/10.22080/jgr.2023.25221.1348 https://doaj.org/article/96cbcb4b3dfa4e3a9d3eb0f4e646c48d |
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ftdoajarticles:oai:doaj.org/article:96cbcb4b3dfa4e3a9d3eb0f4e646c48d 2024-02-04T10:04:30+01:00 Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem Hor Torabi Jafroudi Shirin Jamshidi Soheila Talesh Sasani Ali Bani 2023-02-01T00:00:00Z https://doi.org/10.22080/jgr.2023.25221.1348 https://doaj.org/article/96cbcb4b3dfa4e3a9d3eb0f4e646c48d EN eng University of Mazandaran https://sc.journals.umz.ac.ir/article_4297_26c4045fcae0b69ba1ccf285fe4dabc9.pdf https://doaj.org/toc/2423-4257 https://doaj.org/toc/2588-2589 2423-4257 2588-2589 doi:10.22080/jgr.2023.25221.1348 https://doaj.org/article/96cbcb4b3dfa4e3a9d3eb0f4e646c48d Journal of Genetic Resources, Vol 9, Iss 1, Pp 103-110 (2023) aquatic ecosystem environmental dna persian sturgeon real-time pcr Genetics QH426-470 article 2023 ftdoajarticles https://doi.org/10.22080/jgr.2023.25221.1348 2024-01-07T01:46:21Z Considering the importance of Caspian Sea sturgeon conservation according to CITES rules, finding effective and efficient methods for tracing and identifying sturgeon species are necessary. Residual DNA detection in fish ponds can be used as a model for tracing fish environmental DNA (eDNA) in rivers and seas. This method of DNA detection is non-invasive and advantageous for the conservation of critically endangered sturgeons. Sampling was done from the water of the Persian sturgeon fish pond and fixed with precipitation premix solution (ethanol and acetate sodium). DNA was extracted from fixed water, and fine tissue of two sturgeon species in the Caspian Sea, Sterlet, and Siberian sturgeon. The positive specificity of primers was checked by conventional PCR for sturgeons with fine tissue DNA samples. The linear relationship between the threshold cycle (ct) value and the Persian Sturgeon DNA concentration was measured by the Mini-Barcoding quantitative real-time PCR. DNA samples of fish ponds generated a curve that could not be produced in negative control and irrelevant (non-sturgeon) genomic DNA. Although residual DNA of Persian sturgeons was detected in fish pond water at picogram level through the quantitative method, molecular diagnosis with this method can confirm only the existence of sturgeon species (in general) in fish ponds. In identifying residual DNA in the picogram level of the Persian sturgeon fish pond, SYBER Green real-time PCR method can be an acceptable method for barcoding with high efficiency compared to other methods such as conventional PCR method and non-invasive which is advantageous for the conservation of critically endangered sturgeons. Article in Journal/Newspaper Siberian sturgeon Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
aquatic ecosystem environmental dna persian sturgeon real-time pcr Genetics QH426-470 |
spellingShingle |
aquatic ecosystem environmental dna persian sturgeon real-time pcr Genetics QH426-470 Hor Torabi Jafroudi Shirin Jamshidi Soheila Talesh Sasani Ali Bani Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem |
topic_facet |
aquatic ecosystem environmental dna persian sturgeon real-time pcr Genetics QH426-470 |
description |
Considering the importance of Caspian Sea sturgeon conservation according to CITES rules, finding effective and efficient methods for tracing and identifying sturgeon species are necessary. Residual DNA detection in fish ponds can be used as a model for tracing fish environmental DNA (eDNA) in rivers and seas. This method of DNA detection is non-invasive and advantageous for the conservation of critically endangered sturgeons. Sampling was done from the water of the Persian sturgeon fish pond and fixed with precipitation premix solution (ethanol and acetate sodium). DNA was extracted from fixed water, and fine tissue of two sturgeon species in the Caspian Sea, Sterlet, and Siberian sturgeon. The positive specificity of primers was checked by conventional PCR for sturgeons with fine tissue DNA samples. The linear relationship between the threshold cycle (ct) value and the Persian Sturgeon DNA concentration was measured by the Mini-Barcoding quantitative real-time PCR. DNA samples of fish ponds generated a curve that could not be produced in negative control and irrelevant (non-sturgeon) genomic DNA. Although residual DNA of Persian sturgeons was detected in fish pond water at picogram level through the quantitative method, molecular diagnosis with this method can confirm only the existence of sturgeon species (in general) in fish ponds. In identifying residual DNA in the picogram level of the Persian sturgeon fish pond, SYBER Green real-time PCR method can be an acceptable method for barcoding with high efficiency compared to other methods such as conventional PCR method and non-invasive which is advantageous for the conservation of critically endangered sturgeons. |
format |
Article in Journal/Newspaper |
author |
Hor Torabi Jafroudi Shirin Jamshidi Soheila Talesh Sasani Ali Bani |
author_facet |
Hor Torabi Jafroudi Shirin Jamshidi Soheila Talesh Sasani Ali Bani |
author_sort |
Hor Torabi Jafroudi |
title |
Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem |
title_short |
Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem |
title_full |
Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem |
title_fullStr |
Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem |
title_full_unstemmed |
Molecular Identification of Residual DNA Separated from the Persian Sturgeon (Acipenser persicus) for Modeling eDNA Evaluation in Aquatic Ecosystem |
title_sort |
molecular identification of residual dna separated from the persian sturgeon (acipenser persicus) for modeling edna evaluation in aquatic ecosystem |
publisher |
University of Mazandaran |
publishDate |
2023 |
url |
https://doi.org/10.22080/jgr.2023.25221.1348 https://doaj.org/article/96cbcb4b3dfa4e3a9d3eb0f4e646c48d |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_source |
Journal of Genetic Resources, Vol 9, Iss 1, Pp 103-110 (2023) |
op_relation |
https://sc.journals.umz.ac.ir/article_4297_26c4045fcae0b69ba1ccf285fe4dabc9.pdf https://doaj.org/toc/2423-4257 https://doaj.org/toc/2588-2589 2423-4257 2588-2589 doi:10.22080/jgr.2023.25221.1348 https://doaj.org/article/96cbcb4b3dfa4e3a9d3eb0f4e646c48d |
op_doi |
https://doi.org/10.22080/jgr.2023.25221.1348 |
_version_ |
1789973007366619136 |