Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection
Abstract Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y. Methods The mRNA ex...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:966891e44bd24f1b98d7376e1dbd96e2 2023-05-15T15:10:12+02:00 Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection Mariana Gatto Larissa Ragozo Cardoso Oliveira Fernanda De Nuzzi Dias João Pessoa Araújo Júnior Carlos Roberto Gonçalves Lima Eliana Peresi Lordelo Rodrigo Mattos dos Santos Cilmery Suemi Kurokawa 2017-12-01T00:00:00Z https://doi.org/10.1186/s40409-017-0137-4 https://doaj.org/article/966891e44bd24f1b98d7376e1dbd96e2 EN eng SciELO http://link.springer.com/article/10.1186/s40409-017-0137-4 https://doaj.org/toc/1678-9199 doi:10.1186/s40409-017-0137-4 1678-9199 https://doaj.org/article/966891e44bd24f1b98d7376e1dbd96e2 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-9 (2017) Chagas disease Trypanosoma cruzi Immune response Cytokines q-PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2017 ftdoajarticles https://doi.org/10.1186/s40409-017-0137-4 2022-12-31T14:53:15Z Abstract Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y. Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student’s paired t-test and independent groups were compared using Student’s unpaired t-test. Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and Foxp3 in spleen and heart tissue compared to untreated infected animals. Conclusion Benznidazole can induce Th1 profile in the initial of the acute phase. The treatment decreased the parasite load in both organs, although the number of parasites in Y-strain-infected mice remained high. The data suggest that benznidazole may modulate cytokine expression in infection and can be dependent of the strain. However, treatment was not fully effective in the infection provoked by Y strain, probably due to the characteristics of the strain itself. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 23 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
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Chagas disease Trypanosoma cruzi Immune response Cytokines q-PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Chagas disease Trypanosoma cruzi Immune response Cytokines q-PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Mariana Gatto Larissa Ragozo Cardoso Oliveira Fernanda De Nuzzi Dias João Pessoa Araújo Júnior Carlos Roberto Gonçalves Lima Eliana Peresi Lordelo Rodrigo Mattos dos Santos Cilmery Suemi Kurokawa Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection |
topic_facet |
Chagas disease Trypanosoma cruzi Immune response Cytokines q-PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y. Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student’s paired t-test and independent groups were compared using Student’s unpaired t-test. Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and Foxp3 in spleen and heart tissue compared to untreated infected animals. Conclusion Benznidazole can induce Th1 profile in the initial of the acute phase. The treatment decreased the parasite load in both organs, although the number of parasites in Y-strain-infected mice remained high. The data suggest that benznidazole may modulate cytokine expression in infection and can be dependent of the strain. However, treatment was not fully effective in the infection provoked by Y strain, probably due to the characteristics of the strain itself. |
format |
Article in Journal/Newspaper |
author |
Mariana Gatto Larissa Ragozo Cardoso Oliveira Fernanda De Nuzzi Dias João Pessoa Araújo Júnior Carlos Roberto Gonçalves Lima Eliana Peresi Lordelo Rodrigo Mattos dos Santos Cilmery Suemi Kurokawa |
author_facet |
Mariana Gatto Larissa Ragozo Cardoso Oliveira Fernanda De Nuzzi Dias João Pessoa Araújo Júnior Carlos Roberto Gonçalves Lima Eliana Peresi Lordelo Rodrigo Mattos dos Santos Cilmery Suemi Kurokawa |
author_sort |
Mariana Gatto |
title |
Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection |
title_short |
Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection |
title_full |
Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection |
title_fullStr |
Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection |
title_full_unstemmed |
Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection |
title_sort |
benznidazole affects expression of th1, th17 and treg cytokines during acute experimental trypanosoma cruzi infection |
publisher |
SciELO |
publishDate |
2017 |
url |
https://doi.org/10.1186/s40409-017-0137-4 https://doaj.org/article/966891e44bd24f1b98d7376e1dbd96e2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-9 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s40409-017-0137-4 https://doaj.org/toc/1678-9199 doi:10.1186/s40409-017-0137-4 1678-9199 https://doaj.org/article/966891e44bd24f1b98d7376e1dbd96e2 |
op_doi |
https://doi.org/10.1186/s40409-017-0137-4 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
23 |
container_issue |
1 |
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1766341242614972416 |