Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot
Sera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus...
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ftdoajarticles:oai:doaj.org/article:96666e915ee848d5b54203db3493188b 2024-09-09T19:28:26+00:00 Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot Maria M.I. Ishida Regina Helena S. Peralta José. A. Livramento Sumie Hoshino-Shimizu José M. Peralta Adelaide J. Vaz 2006-12-01T00:00:00Z https://doi.org/10.1590/S0036-46652006000600008 https://doaj.org/article/96666e915ee848d5b54203db3493188b EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652006000600008 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652006000600008 0036-4665 1678-9946 https://doaj.org/article/96666e915ee848d5b54203db3493188b Revista do Instituto de Medicina Tropical de São Paulo, Vol 48, Iss 6, Pp 343-346 (2006) Taenia solium Taenia crassiceps Cysticercosis Serological diagnosis ELISA Immunoblot Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1590/S0036-46652006000600008 2024-08-05T17:49:30Z Sera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy. Amostras de soro de 88 pacientes dos Estados de Santa Catarina e São Paulo, Brasil, com crises epilépticas e que se submeteram a exame de Tomografia Computadorizada (TC), foram examinadas para detecção de anticorpos anti-cisticercos de Taenia solium por meio de ELISA e Immunoblot (IB) utilizando-se os seguintes antígenos: extrato salino total de cisticercos de T. solium (Tso); líquido vesicular de Taenia crassiceps (Tcra-vf) e glicoproteínas purificadas de cisticercos de T. crassiceps (Tcra-gp). Os resultados de ELISA com os antígenos Tso, Tcra-vf e Tcra-gp mostraram 95%, 90% e 80% de sensibilidade, respectivamente, e 68%, 85% e 93% de especificidade, respectivamente. No grupo de pacientes epilépticos, a positividade do ELISA foi 30%, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 48 6 343 346 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Taenia solium Taenia crassiceps Cysticercosis Serological diagnosis ELISA Immunoblot Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Taenia solium Taenia crassiceps Cysticercosis Serological diagnosis ELISA Immunoblot Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Maria M.I. Ishida Regina Helena S. Peralta José. A. Livramento Sumie Hoshino-Shimizu José M. Peralta Adelaide J. Vaz Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot |
topic_facet |
Taenia solium Taenia crassiceps Cysticercosis Serological diagnosis ELISA Immunoblot Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Sera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy. Amostras de soro de 88 pacientes dos Estados de Santa Catarina e São Paulo, Brasil, com crises epilépticas e que se submeteram a exame de Tomografia Computadorizada (TC), foram examinadas para detecção de anticorpos anti-cisticercos de Taenia solium por meio de ELISA e Immunoblot (IB) utilizando-se os seguintes antígenos: extrato salino total de cisticercos de T. solium (Tso); líquido vesicular de Taenia crassiceps (Tcra-vf) e glicoproteínas purificadas de cisticercos de T. crassiceps (Tcra-gp). Os resultados de ELISA com os antígenos Tso, Tcra-vf e Tcra-gp mostraram 95%, 90% e 80% de sensibilidade, respectivamente, e 68%, 85% e 93% de especificidade, respectivamente. No grupo de pacientes epilépticos, a positividade do ELISA foi 30%, ... |
format |
Article in Journal/Newspaper |
author |
Maria M.I. Ishida Regina Helena S. Peralta José. A. Livramento Sumie Hoshino-Shimizu José M. Peralta Adelaide J. Vaz |
author_facet |
Maria M.I. Ishida Regina Helena S. Peralta José. A. Livramento Sumie Hoshino-Shimizu José M. Peralta Adelaide J. Vaz |
author_sort |
Maria M.I. Ishida |
title |
Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot |
title_short |
Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot |
title_full |
Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot |
title_fullStr |
Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot |
title_full_unstemmed |
Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay Sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de ELISA e immunoblot |
title_sort |
serodiagnosis of neurocysticercosis in patients with epileptic seizure using elisa and immunoblot assay sorodiagnóstico da neurocisticercose em pacientes com crises epiléticas, por meio de elisa e immunoblot |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2006 |
url |
https://doi.org/10.1590/S0036-46652006000600008 https://doaj.org/article/96666e915ee848d5b54203db3493188b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 48, Iss 6, Pp 343-346 (2006) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652006000600008 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652006000600008 0036-4665 1678-9946 https://doaj.org/article/96666e915ee848d5b54203db3493188b |
op_doi |
https://doi.org/10.1590/S0036-46652006000600008 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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48 |
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