Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland
Thermophilic bacteria have gained increased attention as candidates for bioethanol production from lignocellulosic biomass. This study investigated ethanol production by Thermoanaerobacter strain J1 from hydrolysates made from lignocellulosic biomass in batch cultures. The effect of increased initia...
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Hindawi Limited
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ftdoajarticles:oai:doaj.org/article:964db2f4be094a908b79b8602a382ba0 2023-05-15T16:50:40+02:00 Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland Jan Eric Jessen Johann Orlygsson 2012-01-01T00:00:00Z https://doi.org/10.1155/2012/186982 https://doaj.org/article/964db2f4be094a908b79b8602a382ba0 EN eng Hindawi Limited http://dx.doi.org/10.1155/2012/186982 https://doaj.org/toc/1110-7243 https://doaj.org/toc/1110-7251 1110-7243 1110-7251 doi:10.1155/2012/186982 https://doaj.org/article/964db2f4be094a908b79b8602a382ba0 Journal of Biomedicine and Biotechnology, Vol 2012 (2012) Biotechnology TP248.13-248.65 Medicine R article 2012 ftdoajarticles https://doi.org/10.1155/2012/186982 2022-12-30T20:51:07Z Thermophilic bacteria have gained increased attention as candidates for bioethanol production from lignocellulosic biomass. This study investigated ethanol production by Thermoanaerobacter strain J1 from hydrolysates made from lignocellulosic biomass in batch cultures. The effect of increased initial glucose concentration and the partial pressure of hydrogen on end product formation were examined. The strain showed a broad substrate spectrum, and high ethanol yields were observed on glucose (1.70 mol/mol) and xylose (1.25 mol/mol). Ethanol yields were, however, dramatically lowered by adding thiosulfate or by cocultivating strain J1 with a hydrogenotrophic methanogen with acetate becoming the major end product. Ethanol production from 4.5 g/L of lignocellulosic biomass hydrolysates (grass, hemp stem, wheat straw, newspaper, and cellulose) pretreated with acid or alkali and the enzymes Celluclast and Novozymes 188 was investigated. The highest ethanol yields were obtained on cellulose (7.5 mM·g−1) but the lowest on straw (0.8 mM·g−1). Chemical pretreatment increased ethanol yields substantially from lignocellulosic biomass but not from cellulose. The largest increase was on straw hydrolysates where ethanol production increased from 0.8 mM·g−1 to 3.3 mM·g−1 using alkali-pretreated biomass. The highest ethanol yields on lignocellulosic hydrolysates were observed with hemp hydrolysates pretreated with acid, 4.2 mM·g−1. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Journal of Biomedicine and Biotechnology 2012 1 7 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Biotechnology TP248.13-248.65 Medicine R |
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Biotechnology TP248.13-248.65 Medicine R Jan Eric Jessen Johann Orlygsson Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland |
topic_facet |
Biotechnology TP248.13-248.65 Medicine R |
description |
Thermophilic bacteria have gained increased attention as candidates for bioethanol production from lignocellulosic biomass. This study investigated ethanol production by Thermoanaerobacter strain J1 from hydrolysates made from lignocellulosic biomass in batch cultures. The effect of increased initial glucose concentration and the partial pressure of hydrogen on end product formation were examined. The strain showed a broad substrate spectrum, and high ethanol yields were observed on glucose (1.70 mol/mol) and xylose (1.25 mol/mol). Ethanol yields were, however, dramatically lowered by adding thiosulfate or by cocultivating strain J1 with a hydrogenotrophic methanogen with acetate becoming the major end product. Ethanol production from 4.5 g/L of lignocellulosic biomass hydrolysates (grass, hemp stem, wheat straw, newspaper, and cellulose) pretreated with acid or alkali and the enzymes Celluclast and Novozymes 188 was investigated. The highest ethanol yields were obtained on cellulose (7.5 mM·g−1) but the lowest on straw (0.8 mM·g−1). Chemical pretreatment increased ethanol yields substantially from lignocellulosic biomass but not from cellulose. The largest increase was on straw hydrolysates where ethanol production increased from 0.8 mM·g−1 to 3.3 mM·g−1 using alkali-pretreated biomass. The highest ethanol yields on lignocellulosic hydrolysates were observed with hemp hydrolysates pretreated with acid, 4.2 mM·g−1. |
format |
Article in Journal/Newspaper |
author |
Jan Eric Jessen Johann Orlygsson |
author_facet |
Jan Eric Jessen Johann Orlygsson |
author_sort |
Jan Eric Jessen |
title |
Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland |
title_short |
Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland |
title_full |
Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland |
title_fullStr |
Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland |
title_full_unstemmed |
Production of Ethanol from Sugars and Lignocellulosic Biomass by Thermoanaerobacter J1 Isolated from a Hot Spring in Iceland |
title_sort |
production of ethanol from sugars and lignocellulosic biomass by thermoanaerobacter j1 isolated from a hot spring in iceland |
publisher |
Hindawi Limited |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/186982 https://doaj.org/article/964db2f4be094a908b79b8602a382ba0 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Journal of Biomedicine and Biotechnology, Vol 2012 (2012) |
op_relation |
http://dx.doi.org/10.1155/2012/186982 https://doaj.org/toc/1110-7243 https://doaj.org/toc/1110-7251 1110-7243 1110-7251 doi:10.1155/2012/186982 https://doaj.org/article/964db2f4be094a908b79b8602a382ba0 |
op_doi |
https://doi.org/10.1155/2012/186982 |
container_title |
Journal of Biomedicine and Biotechnology |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
7 |
_version_ |
1766040784251912192 |