Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992005000300008 https://doaj.org/article/9636617cd0934f76919c7a6b053503a8 |
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ftdoajarticles:oai:doaj.org/article:9636617cd0934f76919c7a6b053503a8 2023-05-15T15:06:04+02:00 Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera 2005-09-01T00:00:00Z https://doi.org/10.1590/S1678-91992005000300008 https://doaj.org/article/9636617cd0934f76919c7a6b053503a8 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000300008 1678-9199 https://doaj.org/article/9636617cd0934f76919c7a6b053503a8 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 3, Pp 299-314 (2005) hyperimmunization irradiation Crotalus durissus terrificus ELISA serum neutralization detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2005 ftdoajarticles https://doi.org/10.1590/S1678-91992005000300008 2022-12-31T00:27:51Z ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 11 3 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
hyperimmunization irradiation Crotalus durissus terrificus ELISA serum neutralization detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
hyperimmunization irradiation Crotalus durissus terrificus ELISA serum neutralization detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
topic_facet |
hyperimmunization irradiation Crotalus durissus terrificus ELISA serum neutralization detoxification Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom. |
format |
Article in Journal/Newspaper |
author |
R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera |
author_facet |
R. S. Ferreira Junior N. Nascimento J. C. Martinez J. B. Alves D. A. Meira B. Barraviera |
author_sort |
R. S. Ferreira Junior |
title |
Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
title_short |
Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
title_full |
Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
title_fullStr |
Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
title_full_unstemmed |
Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
title_sort |
immunization with native and cobalt 60-irradiated crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera |
publisher |
SciELO |
publishDate |
2005 |
url |
https://doi.org/10.1590/S1678-91992005000300008 https://doaj.org/article/9636617cd0934f76919c7a6b053503a8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 3, Pp 299-314 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000300008 1678-9199 https://doaj.org/article/9636617cd0934f76919c7a6b053503a8 |
op_doi |
https://doi.org/10.1590/S1678-91992005000300008 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
11 |
container_issue |
3 |
_version_ |
1766337730153807872 |