Rabies-specific antibodies: measuring surrogates of protection against a fatal disease.
Antibodies play a central role in prophylaxis against many infectious agents. While neutralization is a primary function of antibodies, the Fc- and complement-dependent activities of these multifunctional proteins may also be critical in their ability to provide protection against most viruses. Prot...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0000595 https://doaj.org/article/96239b620db54602b2179bbc3e398e48 |
id |
ftdoajarticles:oai:doaj.org/article:96239b620db54602b2179bbc3e398e48 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:96239b620db54602b2179bbc3e398e48 2023-05-15T15:06:57+02:00 Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. Susan M Moore Cathleen A Hanlon 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000595 https://doaj.org/article/96239b620db54602b2179bbc3e398e48 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2834733?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000595 https://doaj.org/article/96239b620db54602b2179bbc3e398e48 PLoS Neglected Tropical Diseases, Vol 4, Iss 3, p e595 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000595 2022-12-31T16:21:50Z Antibodies play a central role in prophylaxis against many infectious agents. While neutralization is a primary function of antibodies, the Fc- and complement-dependent activities of these multifunctional proteins may also be critical in their ability to provide protection against most viruses. Protection against viral pathogens in vivo is complex, and while virus neutralization--the ability of antibody to inactivate virus infectivity, often measured in vitro--is important, it is often only a partial contributor in protection. The rapid fluorescent focus inhibition test (RFFIT) remains the "gold standard" assay to measure rabies virus-neutralizing antibodies. In addition to neutralization, the rabies-specific antigen-binding activity of antibodies may be measured through enzyme-linked immunosorbent assays (ELISAs), as well as other available methods. For any disease, in selecting the appropriate assay(s) to use to assess antibody titers, assay validation and how they are interpreted are important considerations-but for a fatal disease like rabies, they are of paramount importance. The innate limitations of a one-dimensional laboratory test for rabies antibody measurement, as well as the validation of the method of choice, must be carefully considered in the selection of an assay method and for the interpretation of results that might be construed as a surrogate of protection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 3 e595 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Susan M Moore Cathleen A Hanlon Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Antibodies play a central role in prophylaxis against many infectious agents. While neutralization is a primary function of antibodies, the Fc- and complement-dependent activities of these multifunctional proteins may also be critical in their ability to provide protection against most viruses. Protection against viral pathogens in vivo is complex, and while virus neutralization--the ability of antibody to inactivate virus infectivity, often measured in vitro--is important, it is often only a partial contributor in protection. The rapid fluorescent focus inhibition test (RFFIT) remains the "gold standard" assay to measure rabies virus-neutralizing antibodies. In addition to neutralization, the rabies-specific antigen-binding activity of antibodies may be measured through enzyme-linked immunosorbent assays (ELISAs), as well as other available methods. For any disease, in selecting the appropriate assay(s) to use to assess antibody titers, assay validation and how they are interpreted are important considerations-but for a fatal disease like rabies, they are of paramount importance. The innate limitations of a one-dimensional laboratory test for rabies antibody measurement, as well as the validation of the method of choice, must be carefully considered in the selection of an assay method and for the interpretation of results that might be construed as a surrogate of protection. |
format |
Article in Journal/Newspaper |
author |
Susan M Moore Cathleen A Hanlon |
author_facet |
Susan M Moore Cathleen A Hanlon |
author_sort |
Susan M Moore |
title |
Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
title_short |
Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
title_full |
Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
title_fullStr |
Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
title_full_unstemmed |
Rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
title_sort |
rabies-specific antibodies: measuring surrogates of protection against a fatal disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000595 https://doaj.org/article/96239b620db54602b2179bbc3e398e48 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 3, p e595 (2010) |
op_relation |
http://europepmc.org/articles/PMC2834733?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000595 https://doaj.org/article/96239b620db54602b2179bbc3e398e48 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000595 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
3 |
container_start_page |
e595 |
_version_ |
1766338530073640960 |