Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5
Abstract Background Pseudofactin II is a recently identified biosurfactant secreted by Pseudomonas fluorescens BD5, the strain obtained from freshwater from the Arctic Archipelago of Svalbard. Pseudofactin II is a novel compound identified as cyclic lipopeptide with a palmitic acid connected to the...
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ftdoajarticles:oai:doaj.org/article:960137b5300047fd82303c28d2bed962 2023-05-15T14:28:59+02:00 Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 Janek Tomasz Łukaszewicz Marcin Krasowska Anna 2012-02-01T00:00:00Z https://doi.org/10.1186/1471-2180-12-24 https://doaj.org/article/960137b5300047fd82303c28d2bed962 EN eng BMC http://www.biomedcentral.com/1471-2180/12/24 https://doaj.org/toc/1471-2180 doi:10.1186/1471-2180-12-24 1471-2180 https://doaj.org/article/960137b5300047fd82303c28d2bed962 BMC Microbiology, Vol 12, Iss 1, p 24 (2012) Biosurfactant Lipopeptide Adhesion Biofilm Uropathogenic microorganisms Microbiology QR1-502 article 2012 ftdoajarticles https://doi.org/10.1186/1471-2180-12-24 2022-12-31T01:23:39Z Abstract Background Pseudofactin II is a recently identified biosurfactant secreted by Pseudomonas fluorescens BD5, the strain obtained from freshwater from the Arctic Archipelago of Svalbard. Pseudofactin II is a novel compound identified as cyclic lipopeptide with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety. The C-terminal carboxylic group of the last amino acid forms a lactone with the hydroxyl of Thr3. Adhesion is the first stage of biofilm formation and the best moment for the action of antiadhesive and anti-biofilm compounds. Adsorption of biosurfactants to a surface e.g. glass, polystyrene, silicone modifies its hydrophobicity, interfering with the microbial adhesion and desorption processes. In this study the role and applications of pseudofactin II as a antiadhesive compound has been investigated from medicinal and therapeutic perspectives. Results Pseudofactin II lowered the adhesion to three types of surfaces (glass, polystyrene and silicone) of bacterial strains of five species: Escherichia coli, Enterococcus faecalis, Enterococcus hirae, Staphylococcus epidermidis, Proteus mirabilis and two Candida albicans strains. Pretreatment of a polystyrene surface with 0.5 mg/ml pseudofactin II inhibited bacterial adhesion by 36-90% and that of C. albicans by 92-99%. The same concentration of pseudofactin II dislodged 26-70% of preexisting biofilms grown on previously untreated surfaces. Pseudofactin II also caused a marked inhibition of the initial adhesion of E. faecalis, E. coli, E. hirae and C. albicans strains to silicone urethral catheters. The highest concentration tested (0.5 mg/ml) caused a total growth inhibition of S. epidermidis , partial (18-37%) inhibition of other bacteria and 8-9% inhibition of C. albicans growth. Conclusion Pseudofactin II showed antiadhesive activity against several pathogenic microorganisms which are potential biofilm formers on catheters, implants and internal prostheses. Up to 99% prevention could be achieved by 0.5 mg/ml ... Article in Journal/Newspaper Arctic Archipelago Arctic Svalbard Directory of Open Access Journals: DOAJ Articles Arctic Svalbard BMC Microbiology 12 1 24 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Biosurfactant Lipopeptide Adhesion Biofilm Uropathogenic microorganisms Microbiology QR1-502 |
spellingShingle |
Biosurfactant Lipopeptide Adhesion Biofilm Uropathogenic microorganisms Microbiology QR1-502 Janek Tomasz Łukaszewicz Marcin Krasowska Anna Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 |
topic_facet |
Biosurfactant Lipopeptide Adhesion Biofilm Uropathogenic microorganisms Microbiology QR1-502 |
description |
Abstract Background Pseudofactin II is a recently identified biosurfactant secreted by Pseudomonas fluorescens BD5, the strain obtained from freshwater from the Arctic Archipelago of Svalbard. Pseudofactin II is a novel compound identified as cyclic lipopeptide with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety. The C-terminal carboxylic group of the last amino acid forms a lactone with the hydroxyl of Thr3. Adhesion is the first stage of biofilm formation and the best moment for the action of antiadhesive and anti-biofilm compounds. Adsorption of biosurfactants to a surface e.g. glass, polystyrene, silicone modifies its hydrophobicity, interfering with the microbial adhesion and desorption processes. In this study the role and applications of pseudofactin II as a antiadhesive compound has been investigated from medicinal and therapeutic perspectives. Results Pseudofactin II lowered the adhesion to three types of surfaces (glass, polystyrene and silicone) of bacterial strains of five species: Escherichia coli, Enterococcus faecalis, Enterococcus hirae, Staphylococcus epidermidis, Proteus mirabilis and two Candida albicans strains. Pretreatment of a polystyrene surface with 0.5 mg/ml pseudofactin II inhibited bacterial adhesion by 36-90% and that of C. albicans by 92-99%. The same concentration of pseudofactin II dislodged 26-70% of preexisting biofilms grown on previously untreated surfaces. Pseudofactin II also caused a marked inhibition of the initial adhesion of E. faecalis, E. coli, E. hirae and C. albicans strains to silicone urethral catheters. The highest concentration tested (0.5 mg/ml) caused a total growth inhibition of S. epidermidis , partial (18-37%) inhibition of other bacteria and 8-9% inhibition of C. albicans growth. Conclusion Pseudofactin II showed antiadhesive activity against several pathogenic microorganisms which are potential biofilm formers on catheters, implants and internal prostheses. Up to 99% prevention could be achieved by 0.5 mg/ml ... |
format |
Article in Journal/Newspaper |
author |
Janek Tomasz Łukaszewicz Marcin Krasowska Anna |
author_facet |
Janek Tomasz Łukaszewicz Marcin Krasowska Anna |
author_sort |
Janek Tomasz |
title |
Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 |
title_short |
Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 |
title_full |
Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 |
title_fullStr |
Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 |
title_full_unstemmed |
Antiadhesive activity of the biosurfactant pseudofactin II secreted by the Arctic bacterium Pseudomonas fluorescens BD5 |
title_sort |
antiadhesive activity of the biosurfactant pseudofactin ii secreted by the arctic bacterium pseudomonas fluorescens bd5 |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1471-2180-12-24 https://doaj.org/article/960137b5300047fd82303c28d2bed962 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Archipelago Arctic Svalbard |
genre_facet |
Arctic Archipelago Arctic Svalbard |
op_source |
BMC Microbiology, Vol 12, Iss 1, p 24 (2012) |
op_relation |
http://www.biomedcentral.com/1471-2180/12/24 https://doaj.org/toc/1471-2180 doi:10.1186/1471-2180-12-24 1471-2180 https://doaj.org/article/960137b5300047fd82303c28d2bed962 |
op_doi |
https://doi.org/10.1186/1471-2180-12-24 |
container_title |
BMC Microbiology |
container_volume |
12 |
container_issue |
1 |
container_start_page |
24 |
_version_ |
1766303092349861888 |