Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection.
A century after the discovery of Trypanosoma cruzi in a child living in Lassance, Minas Gerais, Brazil in 1909, many uncertainties remain with respect to factors determining the pathogenesis of Chagas disease (CD). Herein, we simultaneously investigate the contribution of both host and parasite fact...
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ftdoajarticles:oai:doaj.org/article:95e6da8deeb44cf396088e97fac347c4 2023-05-15T15:16:05+02:00 Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. Claudiney Melquíades Rodrigues Helder Magno Silva Valadares Amanda Fortes Francisco Jerusa Marilda Arantes Camila França Campos Andréa Teixeira-Carvalho Olindo Assis Martins-Filho Márcio Sobreira Silva Araujo Rosa Maria Esteves Arantes Egler Chiari Glória Regina Franco Carlos Renato Machado Sérgio Danilo Junho Pena Ana Maria Caetano Faria Andréa Mara Macedo 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000846 https://doaj.org/article/95e6da8deeb44cf396088e97fac347c4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2953483?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000846 https://doaj.org/article/95e6da8deeb44cf396088e97fac347c4 PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e846 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000846 2022-12-31T16:00:01Z A century after the discovery of Trypanosoma cruzi in a child living in Lassance, Minas Gerais, Brazil in 1909, many uncertainties remain with respect to factors determining the pathogenesis of Chagas disease (CD). Herein, we simultaneously investigate the contribution of both host and parasite factors during acute phase of infection in BALB/c mice infected with the JG and/or CL Brener T. cruzi strains. JG single infected mice presented reduced parasitemia and heart parasitism, no mortality, levels of pro-inflammatory mediators (TNF-α, CCL2, IL-6 and IFN-γ) similar to those found among naïve animals and no clinical manifestations of disease. On the other hand, CL Brener single infected mice presented higher parasitemia and heart parasitism, as well as an increased systemic release of pro-inflammatory mediators and higher mortality probably due to a toxic shock-like systemic inflammatory response. Interestingly, coinfection with JG and CL Brener strains resulted in intermediate parasitemia, heart parasitism and mortality. This was accompanied by an increase in the systemic release of IL-10 with a parallel increase in the number of MAC-3(+) and CD4(+) T spleen cells expressing IL-10. Therefore, the endogenous production of IL-10 elicited by coinfection seems to be crucial to counterregulate the potentially lethal effects triggered by systemic release of pro-inflammatory mediators induced by CL Brener single infection. In conclusion, our results suggest that the composition of the infecting parasite population plays a role in the host response to T. cruzi in determining the severity of the disease in experimentally infected BALB/c mice. The combination of JG and CL Brener was able to trigger both protective inflammatory immunity and regulatory immune mechanisms that attenuate damage caused by inflammation and disease severity in BALB/c mice. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 10 e846 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Claudiney Melquíades Rodrigues Helder Magno Silva Valadares Amanda Fortes Francisco Jerusa Marilda Arantes Camila França Campos Andréa Teixeira-Carvalho Olindo Assis Martins-Filho Márcio Sobreira Silva Araujo Rosa Maria Esteves Arantes Egler Chiari Glória Regina Franco Carlos Renato Machado Sérgio Danilo Junho Pena Ana Maria Caetano Faria Andréa Mara Macedo Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
A century after the discovery of Trypanosoma cruzi in a child living in Lassance, Minas Gerais, Brazil in 1909, many uncertainties remain with respect to factors determining the pathogenesis of Chagas disease (CD). Herein, we simultaneously investigate the contribution of both host and parasite factors during acute phase of infection in BALB/c mice infected with the JG and/or CL Brener T. cruzi strains. JG single infected mice presented reduced parasitemia and heart parasitism, no mortality, levels of pro-inflammatory mediators (TNF-α, CCL2, IL-6 and IFN-γ) similar to those found among naïve animals and no clinical manifestations of disease. On the other hand, CL Brener single infected mice presented higher parasitemia and heart parasitism, as well as an increased systemic release of pro-inflammatory mediators and higher mortality probably due to a toxic shock-like systemic inflammatory response. Interestingly, coinfection with JG and CL Brener strains resulted in intermediate parasitemia, heart parasitism and mortality. This was accompanied by an increase in the systemic release of IL-10 with a parallel increase in the number of MAC-3(+) and CD4(+) T spleen cells expressing IL-10. Therefore, the endogenous production of IL-10 elicited by coinfection seems to be crucial to counterregulate the potentially lethal effects triggered by systemic release of pro-inflammatory mediators induced by CL Brener single infection. In conclusion, our results suggest that the composition of the infecting parasite population plays a role in the host response to T. cruzi in determining the severity of the disease in experimentally infected BALB/c mice. The combination of JG and CL Brener was able to trigger both protective inflammatory immunity and regulatory immune mechanisms that attenuate damage caused by inflammation and disease severity in BALB/c mice. |
format |
Article in Journal/Newspaper |
author |
Claudiney Melquíades Rodrigues Helder Magno Silva Valadares Amanda Fortes Francisco Jerusa Marilda Arantes Camila França Campos Andréa Teixeira-Carvalho Olindo Assis Martins-Filho Márcio Sobreira Silva Araujo Rosa Maria Esteves Arantes Egler Chiari Glória Regina Franco Carlos Renato Machado Sérgio Danilo Junho Pena Ana Maria Caetano Faria Andréa Mara Macedo |
author_facet |
Claudiney Melquíades Rodrigues Helder Magno Silva Valadares Amanda Fortes Francisco Jerusa Marilda Arantes Camila França Campos Andréa Teixeira-Carvalho Olindo Assis Martins-Filho Márcio Sobreira Silva Araujo Rosa Maria Esteves Arantes Egler Chiari Glória Regina Franco Carlos Renato Machado Sérgio Danilo Junho Pena Ana Maria Caetano Faria Andréa Mara Macedo |
author_sort |
Claudiney Melquíades Rodrigues |
title |
Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. |
title_short |
Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. |
title_full |
Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. |
title_fullStr |
Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. |
title_full_unstemmed |
Coinfection with different Trypanosoma cruzi strains interferes with the host immune response to infection. |
title_sort |
coinfection with different trypanosoma cruzi strains interferes with the host immune response to infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000846 https://doaj.org/article/95e6da8deeb44cf396088e97fac347c4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e846 (2010) |
op_relation |
http://europepmc.org/articles/PMC2953483?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000846 https://doaj.org/article/95e6da8deeb44cf396088e97fac347c4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000846 |
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PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
10 |
container_start_page |
e846 |
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