Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia
This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006. In the previous study, 926 stool samples were collected from children aged 3–60 months with acute diarrh...
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ftdoajarticles:oai:doaj.org/article:95ab3b5b2c42439f8af1d6f938507086 2024-09-09T19:26:32+00:00 Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia Kaewkanya Nakjarung Ladaporn Bodhidatta Pimmnapar Neesanant Paphavee Lertsethtakarn Orntipa Sethabutr Ket Vansith Chhour Y. Meng Brett E. Swierczewski Carl J. Mason 2016-01-01T00:00:00Z https://doi.org/10.1155/2016/2707121 https://doaj.org/article/95ab3b5b2c42439f8af1d6f938507086 EN eng Wiley http://dx.doi.org/10.1155/2016/2707121 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2016/2707121 https://doaj.org/article/95ab3b5b2c42439f8af1d6f938507086 Journal of Tropical Medicine, Vol 2016 (2016) Arctic medicine. Tropical medicine RC955-962 article 2016 ftdoajarticles https://doi.org/10.1155/2016/2707121 2024-08-05T17:48:44Z This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006. In the previous study, 926 stool samples were collected from children aged 3–60 months with acute diarrhea (cases) and without diarrhea (controls) with reported 6.7% of cases and 3.2% of controls being positive for norovirus. The initial norovirus diagnostic assay was performed with real-time reverse transcription-polymerase chain reaction (real-time RT PCR) which also distinguished between genogroups I and II (GI and GII). Norovirus infection was most commonly detected in children aged 12–23 months in both cases and controls. Norovirus Genotyping Tool and phylogenetic analysis of partial sequences of the 3′ end of the RNA-dependent RNA Polymerase (RdRp) and the capsid domain region were employed to assign genotypes of the norovirus strains. GII.4 was the most predominant capsid genotype detected at 39.5% followed by GII.6 at 14.9%. The GII.4 Hunter 2004 variant was the predominant strain detected. Six RdRP/capsid recombinants including GII.P7/GII.6, GII.P7/GII.14, GII.P7/GII.20, GII.P12/GII.13, GII.P17/GII.16, and GII.P21/GII.3 were also identified. This study of norovirus infection in young children in Cambodia suggests genetic diversity of norovirus as reported worldwide. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2016 1 8 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Kaewkanya Nakjarung Ladaporn Bodhidatta Pimmnapar Neesanant Paphavee Lertsethtakarn Orntipa Sethabutr Ket Vansith Chhour Y. Meng Brett E. Swierczewski Carl J. Mason Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
This study investigated the genetic diversity of noroviruses identified from a previous surveillance study conducted at the National Pediatric Hospital in Phnom Penh, Cambodia, from 2004 to 2006. In the previous study, 926 stool samples were collected from children aged 3–60 months with acute diarrhea (cases) and without diarrhea (controls) with reported 6.7% of cases and 3.2% of controls being positive for norovirus. The initial norovirus diagnostic assay was performed with real-time reverse transcription-polymerase chain reaction (real-time RT PCR) which also distinguished between genogroups I and II (GI and GII). Norovirus infection was most commonly detected in children aged 12–23 months in both cases and controls. Norovirus Genotyping Tool and phylogenetic analysis of partial sequences of the 3′ end of the RNA-dependent RNA Polymerase (RdRp) and the capsid domain region were employed to assign genotypes of the norovirus strains. GII.4 was the most predominant capsid genotype detected at 39.5% followed by GII.6 at 14.9%. The GII.4 Hunter 2004 variant was the predominant strain detected. Six RdRP/capsid recombinants including GII.P7/GII.6, GII.P7/GII.14, GII.P7/GII.20, GII.P12/GII.13, GII.P17/GII.16, and GII.P21/GII.3 were also identified. This study of norovirus infection in young children in Cambodia suggests genetic diversity of norovirus as reported worldwide. |
format |
Article in Journal/Newspaper |
author |
Kaewkanya Nakjarung Ladaporn Bodhidatta Pimmnapar Neesanant Paphavee Lertsethtakarn Orntipa Sethabutr Ket Vansith Chhour Y. Meng Brett E. Swierczewski Carl J. Mason |
author_facet |
Kaewkanya Nakjarung Ladaporn Bodhidatta Pimmnapar Neesanant Paphavee Lertsethtakarn Orntipa Sethabutr Ket Vansith Chhour Y. Meng Brett E. Swierczewski Carl J. Mason |
author_sort |
Kaewkanya Nakjarung |
title |
Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia |
title_short |
Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia |
title_full |
Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia |
title_fullStr |
Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia |
title_full_unstemmed |
Molecular Epidemiology and Genetic Diversity of Norovirus in Young Children in Phnom Penh, Cambodia |
title_sort |
molecular epidemiology and genetic diversity of norovirus in young children in phnom penh, cambodia |
publisher |
Wiley |
publishDate |
2016 |
url |
https://doi.org/10.1155/2016/2707121 https://doaj.org/article/95ab3b5b2c42439f8af1d6f938507086 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2016 (2016) |
op_relation |
http://dx.doi.org/10.1155/2016/2707121 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2016/2707121 https://doaj.org/article/95ab3b5b2c42439f8af1d6f938507086 |
op_doi |
https://doi.org/10.1155/2016/2707121 |
container_title |
Journal of Tropical Medicine |
container_volume |
2016 |
container_start_page |
1 |
op_container_end_page |
8 |
_version_ |
1809896130544140288 |