Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice.
Almost all Echinococcus multilocularis (Em) infections occur in the liver of the intermediate host, causing a lethal zoonotic helminthic disease, alveolar echinococcosis (AE). However, the long non-coding RNAs (lncRNAs) expression profiles of the host and the potential regulatory function of lncRNA...
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ftdoajarticles:oai:doaj.org/article:958c5741e0c34e26b5b7fe67f7776908 2023-05-15T15:12:44+02:00 Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. Xiaofeng Nian Li Li Xusheng Ma Xiurong Li Wenhui Li Nianzhang Zhang John Asekhaen Ohiolei Le Li Guodong Dai Yanhong Liu Hongbin Yan Baoquan Fu Sa Xiao Wanzhong Jia 2022-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010435 https://doaj.org/article/958c5741e0c34e26b5b7fe67f7776908 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010435 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010435 https://doaj.org/article/958c5741e0c34e26b5b7fe67f7776908 PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010435 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010435 2022-12-31T02:24:01Z Almost all Echinococcus multilocularis (Em) infections occur in the liver of the intermediate host, causing a lethal zoonotic helminthic disease, alveolar echinococcosis (AE). However, the long non-coding RNAs (lncRNAs) expression profiles of the host and the potential regulatory function of lncRNA during Em infection are poorly understood. In this study, the profiles of lncRNAs and mRNAs in the liver of mice at different time points after Em infection were explored by microarray. Thirty-one differentially expressed mRNAs (DEMs) and 68 differentially expressed lncRNAs (DELs) were found continuously dysregulated. These DEMs were notably enriched in "antigen processing and presentation", "Th1 and Th2 cell differentiation" and "Th17 cell differentiation" pathways. The potential predicted function of DELs revealed that most DELs might influence Th17 cell differentiation and TGF-β/Smad pathway of host by trans-regulating SMAD3, STAT1, and early growth response (EGR) genes. At 30 days post-infection (dpi), up-regulated DEMs were enriched in Toll-like and RIG-I-like receptor signaling pathways, which were validated by qRT-PCR, Western blotting and downstream cytokines detection. Furthermore, flow cytometric analysis and serum levels of the corresponding cytokines confirmed the changes in cell-mediated immunity in host during Em infection that showed Th1 and Th17-type CD4+ T-cells were predominant at the early infection stage whereas Th2-type CD4+ T-cells were significantly higher at the middle/late stage. Collectively, our study revealed the potential regulatory functions of lncRNAs in modulating host Th cell subsets and provide novel clues in understanding the influence of Em infection on host innate and adaptive immune response. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 5 e0010435 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiaofeng Nian Li Li Xusheng Ma Xiurong Li Wenhui Li Nianzhang Zhang John Asekhaen Ohiolei Le Li Guodong Dai Yanhong Liu Hongbin Yan Baoquan Fu Sa Xiao Wanzhong Jia Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Almost all Echinococcus multilocularis (Em) infections occur in the liver of the intermediate host, causing a lethal zoonotic helminthic disease, alveolar echinococcosis (AE). However, the long non-coding RNAs (lncRNAs) expression profiles of the host and the potential regulatory function of lncRNA during Em infection are poorly understood. In this study, the profiles of lncRNAs and mRNAs in the liver of mice at different time points after Em infection were explored by microarray. Thirty-one differentially expressed mRNAs (DEMs) and 68 differentially expressed lncRNAs (DELs) were found continuously dysregulated. These DEMs were notably enriched in "antigen processing and presentation", "Th1 and Th2 cell differentiation" and "Th17 cell differentiation" pathways. The potential predicted function of DELs revealed that most DELs might influence Th17 cell differentiation and TGF-β/Smad pathway of host by trans-regulating SMAD3, STAT1, and early growth response (EGR) genes. At 30 days post-infection (dpi), up-regulated DEMs were enriched in Toll-like and RIG-I-like receptor signaling pathways, which were validated by qRT-PCR, Western blotting and downstream cytokines detection. Furthermore, flow cytometric analysis and serum levels of the corresponding cytokines confirmed the changes in cell-mediated immunity in host during Em infection that showed Th1 and Th17-type CD4+ T-cells were predominant at the early infection stage whereas Th2-type CD4+ T-cells were significantly higher at the middle/late stage. Collectively, our study revealed the potential regulatory functions of lncRNAs in modulating host Th cell subsets and provide novel clues in understanding the influence of Em infection on host innate and adaptive immune response. |
format |
Article in Journal/Newspaper |
author |
Xiaofeng Nian Li Li Xusheng Ma Xiurong Li Wenhui Li Nianzhang Zhang John Asekhaen Ohiolei Le Li Guodong Dai Yanhong Liu Hongbin Yan Baoquan Fu Sa Xiao Wanzhong Jia |
author_facet |
Xiaofeng Nian Li Li Xusheng Ma Xiurong Li Wenhui Li Nianzhang Zhang John Asekhaen Ohiolei Le Li Guodong Dai Yanhong Liu Hongbin Yan Baoquan Fu Sa Xiao Wanzhong Jia |
author_sort |
Xiaofeng Nian |
title |
Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. |
title_short |
Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. |
title_full |
Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. |
title_fullStr |
Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. |
title_full_unstemmed |
Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice. |
title_sort |
understanding pathogen-host interplay by expression profiles of lncrna and mrna in the liver of echinococcus multilocularis-infected mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010435 https://doaj.org/article/958c5741e0c34e26b5b7fe67f7776908 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 5, p e0010435 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010435 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010435 https://doaj.org/article/958c5741e0c34e26b5b7fe67f7776908 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010435 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
5 |
container_start_page |
e0010435 |
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1766343378421678080 |