Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation.
A Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its diagnostic accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, a conv...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0008402 https://doaj.org/article/957c41d6cf8341b38112db105e8a2f81 |
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ftdoajarticles:oai:doaj.org/article:957c41d6cf8341b38112db105e8a2f81 2023-05-15T15:16:25+02:00 Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. Susana A Besuschio Albert Picado Arturo Muñoz-Calderón Diana P Wehrendt Marisa Fernández Alejandro Benatar Zoraida Diaz-Bello Cecilia Irurtia Israel Cruz Joseph M Ndung'u María L Cafferata Graciela Montenegro Sergio Sosa Estani Raúl H Lucero Belkisyole Alarcón de Noya Silvia A Longhi Alejandro G Schijman 2020-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008402 https://doaj.org/article/957c41d6cf8341b38112db105e8a2f81 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008402 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008402 https://doaj.org/article/957c41d6cf8341b38112db105e8a2f81 PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008402 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008402 2022-12-31T10:07:30Z A Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its diagnostic accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, a convenience series of clinical samples (venous blood treated with EDTA or different stabilizer agents, heel-prick blood in filter paper or cerebrospinal fluid samples (CSF)) from 30 infants born to seropositive mothers (13 with congenital CD and 17 noninfected), four recipients of organs from CD donors, six orally-infected cases after consumption of contaminated guava juice and six CD patients coinfected with HIV at risk of CD reactivation (N = 46 patients, 46 blood samples and 1 CSF sample) were tested by T. cruzi Loopamp kit (Tc LAMP) and standardized quantitative real-time PCR (qPCR). T. cruzi Loopamp accuracy was estimated using the case definition in the different groups as a reference. Cohen's kappa coefficient (κ) was applied to measure the agreement between Tc LAMP (index test) and qPCR (reference test). Sensitivity and specificity of T. cruzi Loopamp kit in blood samples from the pooled clinical groups was 93% (95% CI: 77-99) and 100% (95% CI: 80-100) respectively. The agreement between Tc LAMP and qPCR was almost perfect (κ = 0.92, 95% CI: 0.62-1.00). The T. cruzi Loopamp kit was sensitive and specific for detection of T. cruzi infection. It was carried out from DNA extracted from peripheral blood samples (via frozen EDTA blood, guanidine hydrochloride-EDTA blood, DNAgard blood and dried blood spots), as well as in CSF specimens infected with TcI or TcII/V/VI parasite populations. The T. cruzi Loopamp kit appears potentially useful for rapid detection of T. cruzi infection in congenital, acute and CD reactivation due to HIV infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 8 e0008402 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Susana A Besuschio Albert Picado Arturo Muñoz-Calderón Diana P Wehrendt Marisa Fernández Alejandro Benatar Zoraida Diaz-Bello Cecilia Irurtia Israel Cruz Joseph M Ndung'u María L Cafferata Graciela Montenegro Sergio Sosa Estani Raúl H Lucero Belkisyole Alarcón de Noya Silvia A Longhi Alejandro G Schijman Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
A Trypanosoma cruzi Loopamp kit was recently developed as a ready-to-use diagnostic method requiring minimal laboratory facilities. We evaluated its diagnostic accuracy for detection of acute Chagas disease (CD) in different epidemiological and clinical scenarios. In this retrospective study, a convenience series of clinical samples (venous blood treated with EDTA or different stabilizer agents, heel-prick blood in filter paper or cerebrospinal fluid samples (CSF)) from 30 infants born to seropositive mothers (13 with congenital CD and 17 noninfected), four recipients of organs from CD donors, six orally-infected cases after consumption of contaminated guava juice and six CD patients coinfected with HIV at risk of CD reactivation (N = 46 patients, 46 blood samples and 1 CSF sample) were tested by T. cruzi Loopamp kit (Tc LAMP) and standardized quantitative real-time PCR (qPCR). T. cruzi Loopamp accuracy was estimated using the case definition in the different groups as a reference. Cohen's kappa coefficient (κ) was applied to measure the agreement between Tc LAMP (index test) and qPCR (reference test). Sensitivity and specificity of T. cruzi Loopamp kit in blood samples from the pooled clinical groups was 93% (95% CI: 77-99) and 100% (95% CI: 80-100) respectively. The agreement between Tc LAMP and qPCR was almost perfect (κ = 0.92, 95% CI: 0.62-1.00). The T. cruzi Loopamp kit was sensitive and specific for detection of T. cruzi infection. It was carried out from DNA extracted from peripheral blood samples (via frozen EDTA blood, guanidine hydrochloride-EDTA blood, DNAgard blood and dried blood spots), as well as in CSF specimens infected with TcI or TcII/V/VI parasite populations. The T. cruzi Loopamp kit appears potentially useful for rapid detection of T. cruzi infection in congenital, acute and CD reactivation due to HIV infection. |
format |
Article in Journal/Newspaper |
author |
Susana A Besuschio Albert Picado Arturo Muñoz-Calderón Diana P Wehrendt Marisa Fernández Alejandro Benatar Zoraida Diaz-Bello Cecilia Irurtia Israel Cruz Joseph M Ndung'u María L Cafferata Graciela Montenegro Sergio Sosa Estani Raúl H Lucero Belkisyole Alarcón de Noya Silvia A Longhi Alejandro G Schijman |
author_facet |
Susana A Besuschio Albert Picado Arturo Muñoz-Calderón Diana P Wehrendt Marisa Fernández Alejandro Benatar Zoraida Diaz-Bello Cecilia Irurtia Israel Cruz Joseph M Ndung'u María L Cafferata Graciela Montenegro Sergio Sosa Estani Raúl H Lucero Belkisyole Alarcón de Noya Silvia A Longhi Alejandro G Schijman |
author_sort |
Susana A Besuschio |
title |
Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. |
title_short |
Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. |
title_full |
Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. |
title_fullStr |
Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. |
title_full_unstemmed |
Trypanosoma cruzi loop-mediated isothermal amplification (Trypanosoma cruzi Loopamp) kit for detection of congenital, acute and Chagas disease reactivation. |
title_sort |
trypanosoma cruzi loop-mediated isothermal amplification (trypanosoma cruzi loopamp) kit for detection of congenital, acute and chagas disease reactivation. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008402 https://doaj.org/article/957c41d6cf8341b38112db105e8a2f81 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008402 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008402 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008402 https://doaj.org/article/957c41d6cf8341b38112db105e8a2f81 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008402 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
8 |
container_start_page |
e0008402 |
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