Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In t...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992009000100004 https://doaj.org/article/9561af7e541d4b43a9f15c70f4387e0f |
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ftdoajarticles:oai:doaj.org/article:9561af7e541d4b43a9f15c70f4387e0f 2023-05-15T15:07:19+02:00 Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina S Bustillo H Lucero LC Leiva O Acosta EB Kier Joffé JO Gorodner 2009-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992009000100004 https://doaj.org/article/9561af7e541d4b43a9f15c70f4387e0f EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000100004 1678-9199 https://doaj.org/article/9561af7e541d4b43a9f15c70f4387e0f Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 1, Pp 28-42 (2009) Bothrops alternatus Bothrops diporus cytotoxicity C2C12 apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1590/S1678-91992009000100004 2022-12-31T14:57:04Z Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL) than B. alternatus (CC50: 5.8 µg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Argentina Argentine Journal of Venomous Animals and Toxins including Tropical Diseases 15 1 28 42 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bothrops alternatus Bothrops diporus cytotoxicity C2C12 apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Bothrops alternatus Bothrops diporus cytotoxicity C2C12 apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 S Bustillo H Lucero LC Leiva O Acosta EB Kier Joffé JO Gorodner Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
topic_facet |
Bothrops alternatus Bothrops diporus cytotoxicity C2C12 apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 µg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC50: 2 µg/mL) than B. alternatus (CC50: 5.8 µg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line. |
format |
Article in Journal/Newspaper |
author |
S Bustillo H Lucero LC Leiva O Acosta EB Kier Joffé JO Gorodner |
author_facet |
S Bustillo H Lucero LC Leiva O Acosta EB Kier Joffé JO Gorodner |
author_sort |
S Bustillo |
title |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
title_short |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
title_full |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
title_fullStr |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
title_full_unstemmed |
Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina |
title_sort |
cytotoxicity and morphological analysis of cell death induced by bothrops venoms from the northeast of argentina |
publisher |
SciELO |
publishDate |
2009 |
url |
https://doi.org/10.1590/S1678-91992009000100004 https://doaj.org/article/9561af7e541d4b43a9f15c70f4387e0f |
geographic |
Arctic Argentina Argentine |
geographic_facet |
Arctic Argentina Argentine |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 1, Pp 28-42 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100004 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000100004 1678-9199 https://doaj.org/article/9561af7e541d4b43a9f15c70f4387e0f |
op_doi |
https://doi.org/10.1590/S1678-91992009000100004 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
15 |
container_issue |
1 |
container_start_page |
28 |
op_container_end_page |
42 |
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1766338842560823296 |