Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar )
Ulcer diseases are a recalcitrant issue at Atlantic salmon ( Salmo salar ) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa . However, several bacterial species are frequen...
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Online Access: | https://doi.org/10.3390/microorganisms11071736 https://doaj.org/article/94a178b51d324a86970b9a0c94e6d4c8 |
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ftdoajarticles:oai:doaj.org/article:94a178b51d324a86970b9a0c94e6d4c8 2023-08-20T04:05:13+02:00 Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander 2023-07-01T00:00:00Z https://doi.org/10.3390/microorganisms11071736 https://doaj.org/article/94a178b51d324a86970b9a0c94e6d4c8 EN eng MDPI AG https://www.mdpi.com/2076-2607/11/7/1736 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms11071736 2076-2607 https://doaj.org/article/94a178b51d324a86970b9a0c94e6d4c8 Microorganisms, Vol 11, Iss 1736, p 1736 (2023) Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/microorganisms11071736 2023-07-30T00:35:07Z Ulcer diseases are a recalcitrant issue at Atlantic salmon ( Salmo salar ) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa . However, several bacterial species are frequently isolated from ulcer disease events, and it is unclear if other undescribed pathogens are implicated in ulcer disease in Atlantic salmon. Although different polyvalent vaccines are used against M. viscosa , ulcerative outbreaks are continuously reported in Atlantic salmon in Canada. This study analyzed the phenotypical and genomic characteristics of Vibrio sp. J383 isolated from internal organs of vaccinated farmed Atlantic salmon displaying clinical signs of ulcer disease. Infection assays conducted on vaccinated farmed Atlantic salmon and revealed that Vibrio sp. J383 causes a low level of mortalities when administered intracelomic at doses ranging from 10 7 –10 8 CFU/dose. Vibrio sp. J383 persisted in the blood of infected fish for at least 8 weeks at 10 and 12 °C. Clinical signs of this disease were greatest 12 °C, but no mortality and bacteremia were observed at 16 °C. The Vibrio sp. J383 genome (5,902,734 bp) has two chromosomes of 3,633,265 bp and 2,068,312 bp, respectively, and one large plasmid of 201,166 bp. Phylogenetic and comparative analyses indicated that Vibrio sp. J383 is related to V. splendidus , with 93% identity. Furthermore, the phenotypic analysis showed that there were significant differences between Vibrio sp. J383 and other Vibrio spp, suggesting J383 is a novel Vibrio species adapted to cold temperatures. Article in Journal/Newspaper Atlantic salmon North Atlantic Salmo salar Directory of Open Access Journals: DOAJ Articles Canada Microorganisms 11 7 1736 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease Biology (General) QH301-705.5 |
spellingShingle |
Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease Biology (General) QH301-705.5 Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) |
topic_facet |
Atlantic salmon Vibrio sp. J383 genomics phylogenetics phenotype ulcer disease Biology (General) QH301-705.5 |
description |
Ulcer diseases are a recalcitrant issue at Atlantic salmon ( Salmo salar ) aquaculture cage-sites across the North Atlantic region. Classical ulcerative outbreaks (also called winter ulcer disease) refer to a skin infection caused by Moritella viscosa . However, several bacterial species are frequently isolated from ulcer disease events, and it is unclear if other undescribed pathogens are implicated in ulcer disease in Atlantic salmon. Although different polyvalent vaccines are used against M. viscosa , ulcerative outbreaks are continuously reported in Atlantic salmon in Canada. This study analyzed the phenotypical and genomic characteristics of Vibrio sp. J383 isolated from internal organs of vaccinated farmed Atlantic salmon displaying clinical signs of ulcer disease. Infection assays conducted on vaccinated farmed Atlantic salmon and revealed that Vibrio sp. J383 causes a low level of mortalities when administered intracelomic at doses ranging from 10 7 –10 8 CFU/dose. Vibrio sp. J383 persisted in the blood of infected fish for at least 8 weeks at 10 and 12 °C. Clinical signs of this disease were greatest 12 °C, but no mortality and bacteremia were observed at 16 °C. The Vibrio sp. J383 genome (5,902,734 bp) has two chromosomes of 3,633,265 bp and 2,068,312 bp, respectively, and one large plasmid of 201,166 bp. Phylogenetic and comparative analyses indicated that Vibrio sp. J383 is related to V. splendidus , with 93% identity. Furthermore, the phenotypic analysis showed that there were significant differences between Vibrio sp. J383 and other Vibrio spp, suggesting J383 is a novel Vibrio species adapted to cold temperatures. |
format |
Article in Journal/Newspaper |
author |
Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander |
author_facet |
Maryam Ghasemieshkaftaki Ignacio Vasquez Aria Eshraghi Anthony Kurt Gamperl Javier Santander |
author_sort |
Maryam Ghasemieshkaftaki |
title |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) |
title_short |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) |
title_full |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) |
title_fullStr |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) |
title_full_unstemmed |
Comparative Genomic Analysis of a Novel Vibrio sp. Isolated from an Ulcer Disease Event in Atlantic Salmon ( Salmo salar ) |
title_sort |
comparative genomic analysis of a novel vibrio sp. isolated from an ulcer disease event in atlantic salmon ( salmo salar ) |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/microorganisms11071736 https://doaj.org/article/94a178b51d324a86970b9a0c94e6d4c8 |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_source |
Microorganisms, Vol 11, Iss 1736, p 1736 (2023) |
op_relation |
https://www.mdpi.com/2076-2607/11/7/1736 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms11071736 2076-2607 https://doaj.org/article/94a178b51d324a86970b9a0c94e6d4c8 |
op_doi |
https://doi.org/10.3390/microorganisms11071736 |
container_title |
Microorganisms |
container_volume |
11 |
container_issue |
7 |
container_start_page |
1736 |
_version_ |
1774715693784104960 |