Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator
Background Talitrus saltator is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the Northeast Atlantic and Mediterranean. T. saltator exhibits endogenous locomotor activity rhythms and time-compensated sun and moon orientation, both of which necessitate at least one c...
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ftdoajarticles:oai:doaj.org/article:949e13860b9347d1837a8686b10e4473 2023-10-01T03:58:14+02:00 Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator Joseph F. O’Grady Laura S. Hoelters Martin T. Swain David C. Wilcockson 2016-10-01T00:00:00Z https://doi.org/10.7717/peerj.2555 https://doaj.org/article/949e13860b9347d1837a8686b10e4473 EN eng PeerJ Inc. https://peerj.com/articles/2555.pdf https://peerj.com/articles/2555/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.2555 2167-8359 https://doaj.org/article/949e13860b9347d1837a8686b10e4473 PeerJ, Vol 4, p e2555 (2016) Circadian Crustacean Talitrus saltator Transcriptome Rhythms Medicine R article 2016 ftdoajarticles https://doi.org/10.7717/peerj.2555 2023-09-03T00:47:04Z Background Talitrus saltator is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the Northeast Atlantic and Mediterranean. T. saltator exhibits endogenous locomotor activity rhythms and time-compensated sun and moon orientation, both of which necessitate at least one chronometric mechanism. Whilst their behaviour is well studied, currently there are no descriptions of the underlying molecular components of a biological clock in this animal, and very few in other crustacean species. Methods We harvested brain tissue from animals expressing robust circadian activity rhythms and used homology cloning and Illumina RNAseq approaches to sequence and identify the core circadian clock and clock-related genes in these samples. We assessed the temporal expression of these genes in time-course samples from rhythmic animals using RNAseq. Results We identified a comprehensive suite of circadian clock gene homologues in T. saltator including the ‘core’ clock genes period (Talper), cryptochrome 2 (Talcry2), timeless (Taltim), clock (Talclk), and bmal1 (Talbmal1). In addition we describe the sequence and putative structures of 23 clock-associated genes including two unusual, extended isoforms of pigment dispersing hormone (Talpdh). We examined time-course RNAseq expression data, derived from tissues harvested from behaviourally rhythmic animals, to reveal rhythmic expression of these genes with approximately circadian period in Talper and Talbmal1. Of the clock-related genes, casein kinase IIβ (TalckIIβ), ebony (Talebony), jetlag (Taljetlag), pigment dispensing hormone (Talpdh), protein phosphatase 1 (Talpp1), shaggy (Talshaggy), sirt1 (Talsirt1), sirt7 (Talsirt7) and supernumerary limbs (Talslimb) show temporal changes in expression. Discussion We report the sequences of principle genes that comprise the circadian clock of T. saltator and highlight the conserved structural and functional domains of their deduced cognate proteins. Our sequencing data contribute to the growing inventory of ... Article in Journal/Newspaper Northeast Atlantic Directory of Open Access Journals: DOAJ Articles PeerJ 4 e2555 |
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op_collection_id |
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language |
English |
topic |
Circadian Crustacean Talitrus saltator Transcriptome Rhythms Medicine R |
spellingShingle |
Circadian Crustacean Talitrus saltator Transcriptome Rhythms Medicine R Joseph F. O’Grady Laura S. Hoelters Martin T. Swain David C. Wilcockson Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator |
topic_facet |
Circadian Crustacean Talitrus saltator Transcriptome Rhythms Medicine R |
description |
Background Talitrus saltator is an amphipod crustacean that inhabits the supralittoral zone on sandy beaches in the Northeast Atlantic and Mediterranean. T. saltator exhibits endogenous locomotor activity rhythms and time-compensated sun and moon orientation, both of which necessitate at least one chronometric mechanism. Whilst their behaviour is well studied, currently there are no descriptions of the underlying molecular components of a biological clock in this animal, and very few in other crustacean species. Methods We harvested brain tissue from animals expressing robust circadian activity rhythms and used homology cloning and Illumina RNAseq approaches to sequence and identify the core circadian clock and clock-related genes in these samples. We assessed the temporal expression of these genes in time-course samples from rhythmic animals using RNAseq. Results We identified a comprehensive suite of circadian clock gene homologues in T. saltator including the ‘core’ clock genes period (Talper), cryptochrome 2 (Talcry2), timeless (Taltim), clock (Talclk), and bmal1 (Talbmal1). In addition we describe the sequence and putative structures of 23 clock-associated genes including two unusual, extended isoforms of pigment dispersing hormone (Talpdh). We examined time-course RNAseq expression data, derived from tissues harvested from behaviourally rhythmic animals, to reveal rhythmic expression of these genes with approximately circadian period in Talper and Talbmal1. Of the clock-related genes, casein kinase IIβ (TalckIIβ), ebony (Talebony), jetlag (Taljetlag), pigment dispensing hormone (Talpdh), protein phosphatase 1 (Talpp1), shaggy (Talshaggy), sirt1 (Talsirt1), sirt7 (Talsirt7) and supernumerary limbs (Talslimb) show temporal changes in expression. Discussion We report the sequences of principle genes that comprise the circadian clock of T. saltator and highlight the conserved structural and functional domains of their deduced cognate proteins. Our sequencing data contribute to the growing inventory of ... |
format |
Article in Journal/Newspaper |
author |
Joseph F. O’Grady Laura S. Hoelters Martin T. Swain David C. Wilcockson |
author_facet |
Joseph F. O’Grady Laura S. Hoelters Martin T. Swain David C. Wilcockson |
author_sort |
Joseph F. O’Grady |
title |
Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator |
title_short |
Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator |
title_full |
Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator |
title_fullStr |
Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator |
title_full_unstemmed |
Identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean Talitrus saltator |
title_sort |
identification and temporal expression of putative circadian clock transcripts in the amphipod crustacean talitrus saltator |
publisher |
PeerJ Inc. |
publishDate |
2016 |
url |
https://doi.org/10.7717/peerj.2555 https://doaj.org/article/949e13860b9347d1837a8686b10e4473 |
genre |
Northeast Atlantic |
genre_facet |
Northeast Atlantic |
op_source |
PeerJ, Vol 4, p e2555 (2016) |
op_relation |
https://peerj.com/articles/2555.pdf https://peerj.com/articles/2555/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.2555 2167-8359 https://doaj.org/article/949e13860b9347d1837a8686b10e4473 |
op_doi |
https://doi.org/10.7717/peerj.2555 |
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PeerJ |
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4 |
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e2555 |
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1778530800122200064 |