Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses.
BACKGROUND:Vibrio cholerae causes over 2 million cases of cholera and 90,000 deaths each year. Serosurveillance can be a useful tool for estimating the intensity of cholera transmission and prioritizing populations for cholera control interventions. Current methods involving venous blood draws and d...
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ftdoajarticles:oai:doaj.org/article:9497cb6bb6a74c5a80bd7b957f09e6ae 2023-05-15T15:12:34+02:00 Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. Anita S Iyer Andrew S Azman Malika Bouhenia Lul O Deng Cole P Anderson Michael Graves Pavol Kováč Peng Xu Edward T Ryan Jason B Harris David A Sack Francisco J Luquero Daniel T Leung 2018-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006196 https://doaj.org/article/9497cb6bb6a74c5a80bd7b957f09e6ae EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5805362?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006196 https://doaj.org/article/9497cb6bb6a74c5a80bd7b957f09e6ae PLoS Neglected Tropical Diseases, Vol 12, Iss 1, p e0006196 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006196 2022-12-30T21:54:52Z BACKGROUND:Vibrio cholerae causes over 2 million cases of cholera and 90,000 deaths each year. Serosurveillance can be a useful tool for estimating the intensity of cholera transmission and prioritizing populations for cholera control interventions. Current methods involving venous blood draws and downstream specimen storage and transport methods pose logistical challenges in most settings where cholera strikes. To overcome these challenges, we developed methods for determining cholera-specific immune responses from dried blood spots (DBS). METHODOLOGY/PRINCIPAL FINDINGS:As conventional vibriocidal assay methods were unsuitable for DBS eluates from filter paper, we adopted a drop-plate culture method. We show that DBS collected from volunteers in South Sudan, and stored for prolonged periods in field conditions, retained functional vibriocidal antibodies, the titers of which correlated with paired serum titers determined by conventional spectrophotometric methods (r = 0.94, p = 0.00012). We also showed that eluates from DBS Serum Separator cards could be used with conventional spectrophotometric vibriocidal methods, and that they correlated with paired serum at a wide range of titers (r = 0.96, p<0.0001). Similarly, we used ELISA methods to show that V. cholerae O-specific polysaccharide antibody responses from DBS eluates correlated with results from paired serum for IgG (r = 0.85, p = 0.00006), IgM (r = 0.79, p = 0.00049) and IgA (r = 0.73, p = 0.0019), highlighting its potential for use in determination of isotype-specific responses. Storage of DBS cards at a range of temperatures did not change antibody responses. CONCLUSION:In conclusion, we have developed and demonstrated a proof-of-concept for assays utilizing DBS for assessing cholera-specific immune responses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 1 e0006196 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Anita S Iyer Andrew S Azman Malika Bouhenia Lul O Deng Cole P Anderson Michael Graves Pavol Kováč Peng Xu Edward T Ryan Jason B Harris David A Sack Francisco J Luquero Daniel T Leung Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Vibrio cholerae causes over 2 million cases of cholera and 90,000 deaths each year. Serosurveillance can be a useful tool for estimating the intensity of cholera transmission and prioritizing populations for cholera control interventions. Current methods involving venous blood draws and downstream specimen storage and transport methods pose logistical challenges in most settings where cholera strikes. To overcome these challenges, we developed methods for determining cholera-specific immune responses from dried blood spots (DBS). METHODOLOGY/PRINCIPAL FINDINGS:As conventional vibriocidal assay methods were unsuitable for DBS eluates from filter paper, we adopted a drop-plate culture method. We show that DBS collected from volunteers in South Sudan, and stored for prolonged periods in field conditions, retained functional vibriocidal antibodies, the titers of which correlated with paired serum titers determined by conventional spectrophotometric methods (r = 0.94, p = 0.00012). We also showed that eluates from DBS Serum Separator cards could be used with conventional spectrophotometric vibriocidal methods, and that they correlated with paired serum at a wide range of titers (r = 0.96, p<0.0001). Similarly, we used ELISA methods to show that V. cholerae O-specific polysaccharide antibody responses from DBS eluates correlated with results from paired serum for IgG (r = 0.85, p = 0.00006), IgM (r = 0.79, p = 0.00049) and IgA (r = 0.73, p = 0.0019), highlighting its potential for use in determination of isotype-specific responses. Storage of DBS cards at a range of temperatures did not change antibody responses. CONCLUSION:In conclusion, we have developed and demonstrated a proof-of-concept for assays utilizing DBS for assessing cholera-specific immune responses. |
format |
Article in Journal/Newspaper |
author |
Anita S Iyer Andrew S Azman Malika Bouhenia Lul O Deng Cole P Anderson Michael Graves Pavol Kováč Peng Xu Edward T Ryan Jason B Harris David A Sack Francisco J Luquero Daniel T Leung |
author_facet |
Anita S Iyer Andrew S Azman Malika Bouhenia Lul O Deng Cole P Anderson Michael Graves Pavol Kováč Peng Xu Edward T Ryan Jason B Harris David A Sack Francisco J Luquero Daniel T Leung |
author_sort |
Anita S Iyer |
title |
Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. |
title_short |
Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. |
title_full |
Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. |
title_fullStr |
Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. |
title_full_unstemmed |
Dried Blood Spots for Measuring Vibrio cholerae-specific Immune Responses. |
title_sort |
dried blood spots for measuring vibrio cholerae-specific immune responses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006196 https://doaj.org/article/9497cb6bb6a74c5a80bd7b957f09e6ae |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 1, p e0006196 (2018) |
op_relation |
http://europepmc.org/articles/PMC5805362?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006196 https://doaj.org/article/9497cb6bb6a74c5a80bd7b957f09e6ae |
op_doi |
https://doi.org/10.1371/journal.pntd.0006196 |
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PLOS Neglected Tropical Diseases |
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12 |
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1 |
container_start_page |
e0006196 |
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