INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of...
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ftdoajarticles:oai:doaj.org/article:945af429da614ebeb1805e355dce70e0 2023-05-15T15:08:13+02:00 Luciane Cristina Gelatti Renan Rangel Bonamigo Fernanda Matsiko Inoue Mirian Silva do Carmo Ana Paula Becker Fernanda Marques da Silva Castrucci Antônio Carlos Campos Pignatari Pedro Alves d' Azevedo 2013-01-01T00:00:00Z https://doaj.org/article/945af429da614ebeb1805e355dce70e0 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/945af429da614ebeb1805e355dce70e0 Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 1, Pp 34-38 (2013) Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles 2022-12-30T23:08:28Z INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Valentine ENVELOPE(-54.550,-54.550,-61.083,-61.083) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections Arctic medicine. Tropical medicine RC955-962 Luciane Cristina Gelatti Renan Rangel Bonamigo Fernanda Matsiko Inoue Mirian Silva do Carmo Ana Paula Becker Fernanda Marques da Silva Castrucci Antônio Carlos Campos Pignatari Pedro Alves d' Azevedo |
topic_facet |
Staphylococcus aureus Methicillin-resistant Skin infections Soft tissue infections Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen commonly associated with nosocomial infections. However, it has also been associated with community-acquired skin and soft tissue infections (CA-MRSA). There are few data on the identification and prevalence of CA-MRSA infections in Brazil. METHODS: This is a cross-sectional study of 104 patients with community-acquired skin infections attending two health care centers in Porto Alegre, southern Brazil. MRSA isolates were characterized by molecular methods, including detection of the mecA gene by PCR, gene SCCmec typing, Panton-Valentine leukocidin (PVL) detection, pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST). RESULTS: From the 104 samples, 58 Staphylococcus aureus isolates were obtained, of which five (8.6%) had a CA-MRSA-resistant profile. All five isolates had the mecA gene and amplified to SCCmec type IV. Analysis of chromosomal DNA by PFGE revealed the presence of two clusters related to international clones (OSPC and USA 300), with a Dice similarity coefficient >80%. The study was complemented by MLST, which detected three different strains: ST30, ST8, and ST45, the latter not presenting any relation with the clones compared in PFGE. CONCLUSIONS: The presence of CA-MRSA reveals an important change in the epidemiology of this pathogen and adds new elements to the knowledge of the molecular biology of infections by MRSA with SCCmec type IV in southern Brazil. |
format |
Article in Journal/Newspaper |
author |
Luciane Cristina Gelatti Renan Rangel Bonamigo Fernanda Matsiko Inoue Mirian Silva do Carmo Ana Paula Becker Fernanda Marques da Silva Castrucci Antônio Carlos Campos Pignatari Pedro Alves d' Azevedo |
author_facet |
Luciane Cristina Gelatti Renan Rangel Bonamigo Fernanda Matsiko Inoue Mirian Silva do Carmo Ana Paula Becker Fernanda Marques da Silva Castrucci Antônio Carlos Campos Pignatari Pedro Alves d' Azevedo |
author_sort |
Luciane Cristina Gelatti |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2013 |
url |
https://doaj.org/article/945af429da614ebeb1805e355dce70e0 |
long_lat |
ENVELOPE(-54.550,-54.550,-61.083,-61.083) |
geographic |
Arctic Valentine |
geographic_facet |
Arctic Valentine |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 1, Pp 34-38 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000100034 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/945af429da614ebeb1805e355dce70e0 |
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1766339623951269888 |