Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela
Abstract: During the period June 1993 to June 1995, 377 fecal samples from hospitalized young children with extreme diarrhea in the Antonio Patricio de Alcala Hospital in Cumana, Venezuela were taken, as well as similar samples from 89 asymptomatic children of the same age range. Viral ARN was extra...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2003
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ftdoajarticles:oai:doaj.org/article:942db81449e4464abc125d439936ec88 2023-10-01T03:54:23+02:00 Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela Rojas Yacqueline J Bastardo Jesús W Sulbarán María Z 2003-06-01T00:00:00Z https://doaj.org/article/942db81449e4464abc125d439936ec88 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://drive.google.com/file/d/1aeyMreiJJFpf_hCmLqB79ss2VOVD45ee/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/942db81449e4464abc125d439936ec88 Kasmera, Vol 31, Iss 1, Pp 20-28 (2003) Rotavirus seasonal prevalence electro-ferrotype climate epidemiology diarrhea prevalencia estacional electroferotipos clima epidemiología diarrea Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2003 ftdoajarticles 2023-09-03T00:45:10Z Abstract: During the period June 1993 to June 1995, 377 fecal samples from hospitalized young children with extreme diarrhea in the Antonio Patricio de Alcala Hospital in Cumana, Venezuela were taken, as well as similar samples from 89 asymptomatic children of the same age range. Viral ARN was extracted from the samples with phenol-chloroform, ethanol precipitation, gel electrophoresis of poly-acrylamide (Page) and silver tincture. Rotavirus was found in 116 (38.77%) of the diarrhea samples and in 7 (7.87%) of the non-diarrhea samples. Only in 105 of the diarrhea samples was it possible to detect electro-ferrotype patterns, in which 84 (80%) were the long electro-ferrotype; 19 (18.09%) were short electro-ferrotype; and two (1.91%), had mixed electro-ferrotypes. In the non-diarrhea 5 (71.43%) were the long type, and 2 (28.57%) were short. Using co-electrophoresis, 7 electro-ferrotype patterns were distinguished: four long, two short, and one electro-ferrotype mixture. A significant inverse correlation was found between the monthly prevalence of rotavirus and the mean temperature (r=-0.5583, p< 0.05) and a significant inverse relationship between the monthly prevalence of rotavirus and average monthly precipitation. Resumen: Durante el período de junio de 1993 a junio de 1995, se recolectaron 377 muestras de heces de niños menores de cinco años, con diarrea aguda, recluidos en el Hospital Antonio Patricio de Alcalá de la ciudad de Cumaná, Venezuela, y de 89 niños asintomáticos con el mismo rango de edades. A las muestras se les realizó extracción del ARN viral con fenol-cloroformo, precipitación con etanol, electroforesis en geles de poliacrilamida (PAGE) y tinción con plata. Se encontró rotavirus en 116 (30,77%) de las muestras diarreicas y en 7 (7,87%) de las muestras no diarreicas. A sólo 105 de las muestras diarreicas fue posible observarle un patrón electroforético definido, de las cuales, ochenta y cuatro (80%) presentaron electroferotipo largo; diecinueve (18,09%) presentaron electroferotipo corto; y dos ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Arctic Corto ENVELOPE(-60.783,-60.783,-62.450,-62.450) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Rotavirus seasonal prevalence electro-ferrotype climate epidemiology diarrhea prevalencia estacional electroferotipos clima epidemiología diarrea Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Rotavirus seasonal prevalence electro-ferrotype climate epidemiology diarrhea prevalencia estacional electroferotipos clima epidemiología diarrea Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rojas Yacqueline J Bastardo Jesús W Sulbarán María Z Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela |
topic_facet |
Rotavirus seasonal prevalence electro-ferrotype climate epidemiology diarrhea prevalencia estacional electroferotipos clima epidemiología diarrea Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract: During the period June 1993 to June 1995, 377 fecal samples from hospitalized young children with extreme diarrhea in the Antonio Patricio de Alcala Hospital in Cumana, Venezuela were taken, as well as similar samples from 89 asymptomatic children of the same age range. Viral ARN was extracted from the samples with phenol-chloroform, ethanol precipitation, gel electrophoresis of poly-acrylamide (Page) and silver tincture. Rotavirus was found in 116 (38.77%) of the diarrhea samples and in 7 (7.87%) of the non-diarrhea samples. Only in 105 of the diarrhea samples was it possible to detect electro-ferrotype patterns, in which 84 (80%) were the long electro-ferrotype; 19 (18.09%) were short electro-ferrotype; and two (1.91%), had mixed electro-ferrotypes. In the non-diarrhea 5 (71.43%) were the long type, and 2 (28.57%) were short. Using co-electrophoresis, 7 electro-ferrotype patterns were distinguished: four long, two short, and one electro-ferrotype mixture. A significant inverse correlation was found between the monthly prevalence of rotavirus and the mean temperature (r=-0.5583, p< 0.05) and a significant inverse relationship between the monthly prevalence of rotavirus and average monthly precipitation. Resumen: Durante el período de junio de 1993 a junio de 1995, se recolectaron 377 muestras de heces de niños menores de cinco años, con diarrea aguda, recluidos en el Hospital Antonio Patricio de Alcalá de la ciudad de Cumaná, Venezuela, y de 89 niños asintomáticos con el mismo rango de edades. A las muestras se les realizó extracción del ARN viral con fenol-cloroformo, precipitación con etanol, electroforesis en geles de poliacrilamida (PAGE) y tinción con plata. Se encontró rotavirus en 116 (30,77%) de las muestras diarreicas y en 7 (7,87%) de las muestras no diarreicas. A sólo 105 de las muestras diarreicas fue posible observarle un patrón electroforético definido, de las cuales, ochenta y cuatro (80%) presentaron electroferotipo largo; diecinueve (18,09%) presentaron electroferotipo corto; y dos ... |
format |
Article in Journal/Newspaper |
author |
Rojas Yacqueline J Bastardo Jesús W Sulbarán María Z |
author_facet |
Rojas Yacqueline J Bastardo Jesús W Sulbarán María Z |
author_sort |
Rojas Yacqueline J |
title |
Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela |
title_short |
Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela |
title_full |
Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela |
title_fullStr |
Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela |
title_full_unstemmed |
Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela |
title_sort |
prevalencia de rotavirus y su relación con factores climáticos en cumaná, venezuela |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2003 |
url |
https://doaj.org/article/942db81449e4464abc125d439936ec88 |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) ENVELOPE(-60.783,-60.783,-62.450,-62.450) |
geographic |
Aguda Arctic Corto |
geographic_facet |
Aguda Arctic Corto |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 31, Iss 1, Pp 20-28 (2003) |
op_relation |
https://drive.google.com/file/d/1aeyMreiJJFpf_hCmLqB79ss2VOVD45ee/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/942db81449e4464abc125d439936ec88 |
_version_ |
1778521956793974784 |