Prevalencia de rotavirus y su relación con factores climáticos en Cumaná, Venezuela
Abstract: During the period June 1993 to June 1995, 377 fecal samples from hospitalized young children with extreme diarrhea in the Antonio Patricio de Alcala Hospital in Cumana, Venezuela were taken, as well as similar samples from 89 asymptomatic children of the same age range. Viral ARN was extra...
| Main Authors: | , , |
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| Format: | Article in Journal/Newspaper |
| Language: | English Spanish |
| Published: |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2003
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| Subjects: | |
| Online Access: | https://doaj.org/article/942db81449e4464abc125d439936ec88 |
| Summary: | Abstract: During the period June 1993 to June 1995, 377 fecal samples from hospitalized young children with extreme diarrhea in the Antonio Patricio de Alcala Hospital in Cumana, Venezuela were taken, as well as similar samples from 89 asymptomatic children of the same age range. Viral ARN was extracted from the samples with phenol-chloroform, ethanol precipitation, gel electrophoresis of poly-acrylamide (Page) and silver tincture. Rotavirus was found in 116 (38.77%) of the diarrhea samples and in 7 (7.87%) of the non-diarrhea samples. Only in 105 of the diarrhea samples was it possible to detect electro-ferrotype patterns, in which 84 (80%) were the long electro-ferrotype; 19 (18.09%) were short electro-ferrotype; and two (1.91%), had mixed electro-ferrotypes. In the non-diarrhea 5 (71.43%) were the long type, and 2 (28.57%) were short. Using co-electrophoresis, 7 electro-ferrotype patterns were distinguished: four long, two short, and one electro-ferrotype mixture. A significant inverse correlation was found between the monthly prevalence of rotavirus and the mean temperature (r=-0.5583, p< 0.05) and a significant inverse relationship between the monthly prevalence of rotavirus and average monthly precipitation. Resumen: Durante el período de junio de 1993 a junio de 1995, se recolectaron 377 muestras de heces de niños menores de cinco años, con diarrea aguda, recluidos en el Hospital Antonio Patricio de Alcalá de la ciudad de Cumaná, Venezuela, y de 89 niños asintomáticos con el mismo rango de edades. A las muestras se les realizó extracción del ARN viral con fenol-cloroformo, precipitación con etanol, electroforesis en geles de poliacrilamida (PAGE) y tinción con plata. Se encontró rotavirus en 116 (30,77%) de las muestras diarreicas y en 7 (7,87%) de las muestras no diarreicas. A sólo 105 de las muestras diarreicas fue posible observarle un patrón electroforético definido, de las cuales, ochenta y cuatro (80%) presentaron electroferotipo largo; diecinueve (18,09%) presentaron electroferotipo corto; y dos ... |
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