The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products
Scarce research into the secondary metabolites of the fungi Pseudogymnoascus spp. has shown a hidden biosynthetic potential for biologically active compounds. This work investigated the biosynthesis of secondary metabolites by two Pseudogymnoascus fungal strains, VKM F-4518 and VKM F-4519, isolated...
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ftdoajarticles:oai:doaj.org/article:942ae0d9f195445693961df68c48940e 2023-09-26T15:14:30+02:00 The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein 2023-07-01T00:00:00Z https://doi.org/10.3390/fermentation9080702 https://doaj.org/article/942ae0d9f195445693961df68c48940e EN eng MDPI AG https://www.mdpi.com/2311-5637/9/8/702 https://doaj.org/toc/2311-5637 doi:10.3390/fermentation9080702 2311-5637 https://doaj.org/article/942ae0d9f195445693961df68c48940e Fermentation, Vol 9, Iss 702, p 702 (2023) fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B Fermentation industries. Beverages. Alcohol TP500-660 article 2023 ftdoajarticles https://doi.org/10.3390/fermentation9080702 2023-08-27T00:35:43Z Scarce research into the secondary metabolites of the fungi Pseudogymnoascus spp. has shown a hidden biosynthetic potential for biologically active compounds. This work investigated the biosynthesis of secondary metabolites by two Pseudogymnoascus fungal strains, VKM F-4518 and VKM F-4519, isolated from the surface soil layer of the Kolyma Lowland, Russia, in the Arctic. In these strains, 16-membered trilactone macrolides, (+)-macrosphelides A and B, were identified using 1D and 2D NMR, UHRMS, and optical rotation data. In the fungi of this genus, these metabolites were found for the first time. The studied strains are highly active producers of macrosphelide A, which is being considered as a promising agent for the cure of cancer. Using the antiSMASH secondary metabolite analysis tool, we found that the genome of strain VKM F-4518 contained 32 of the biosynthetic clusters of the secondary metabolite genes (BGC) and that of VKM F-4519 had 17 BGCs. Based on the comparison of the cluster of macrotriolide genes from the fungus Paraphaeosphaeria sporulosa , we found the complete supposed cluster BGCs of macrosphelides in the genomes of two Pseudogymnoascus strains using the BLAST+ program. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Kolyma ENVELOPE(161.000,161.000,69.500,69.500) Fermentation 9 8 702 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B Fermentation industries. Beverages. Alcohol TP500-660 |
spellingShingle |
fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B Fermentation industries. Beverages. Alcohol TP500-660 Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
topic_facet |
fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B Fermentation industries. Beverages. Alcohol TP500-660 |
description |
Scarce research into the secondary metabolites of the fungi Pseudogymnoascus spp. has shown a hidden biosynthetic potential for biologically active compounds. This work investigated the biosynthesis of secondary metabolites by two Pseudogymnoascus fungal strains, VKM F-4518 and VKM F-4519, isolated from the surface soil layer of the Kolyma Lowland, Russia, in the Arctic. In these strains, 16-membered trilactone macrolides, (+)-macrosphelides A and B, were identified using 1D and 2D NMR, UHRMS, and optical rotation data. In the fungi of this genus, these metabolites were found for the first time. The studied strains are highly active producers of macrosphelide A, which is being considered as a promising agent for the cure of cancer. Using the antiSMASH secondary metabolite analysis tool, we found that the genome of strain VKM F-4518 contained 32 of the biosynthetic clusters of the secondary metabolite genes (BGC) and that of VKM F-4519 had 17 BGCs. Based on the comparison of the cluster of macrotriolide genes from the fungus Paraphaeosphaeria sporulosa , we found the complete supposed cluster BGCs of macrosphelides in the genomes of two Pseudogymnoascus strains using the BLAST+ program. |
format |
Article in Journal/Newspaper |
author |
Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein |
author_facet |
Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein |
author_sort |
Tatiana V. Antipova |
title |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_short |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_full |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_fullStr |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_full_unstemmed |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_sort |
potential of arctic pseudogymnoascus fungi in the biosynthesis of natural products |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/fermentation9080702 https://doaj.org/article/942ae0d9f195445693961df68c48940e |
long_lat |
ENVELOPE(161.000,161.000,69.500,69.500) |
geographic |
Arctic Kolyma |
geographic_facet |
Arctic Kolyma |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Fermentation, Vol 9, Iss 702, p 702 (2023) |
op_relation |
https://www.mdpi.com/2311-5637/9/8/702 https://doaj.org/toc/2311-5637 doi:10.3390/fermentation9080702 2311-5637 https://doaj.org/article/942ae0d9f195445693961df68c48940e |
op_doi |
https://doi.org/10.3390/fermentation9080702 |
container_title |
Fermentation |
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9 |
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8 |
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702 |
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1778135340247154688 |