Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens
Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxin...
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ftdoajarticles:oai:doaj.org/article:9414ebc8d77349f0b36d721906adf1d6 2023-05-15T15:07:53+02:00 Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens A Tolooe B Shojadoost SM Peighambari 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000100008 https://doaj.org/article/9414ebc8d77349f0b36d721906adf1d6 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100008 1678-9199 https://doaj.org/article/9414ebc8d77349f0b36d721906adf1d6 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 59-65 (2011) Clostridium perfringens necrotic enteritis chicken beta2 toxin cpb2 Iran Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000100008 2022-12-31T02:26:47Z Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxins produced by some of its strains, such as beta2 toxin. The exact role of beta2 toxin in NE pathogenesis is still controversial. In the present study, C. perfringens isolates from healthy and diseased poultry flocks from different parts of Iran were analyzed by PCR assay to determine the presence of all variants of the beta2 toxin gene (cpb2). The products of two positive cpb2 PCR reactions were sequenced, compared to each other and to the cpb2 sequences published in GenBank (by multiple alignment and phylogenetic analysis). The current work represents the first study of cpb2 in poultry C. perfringens isolates in Asia, and reports the highest percentage of cpb2-positive isolates in both apparently healthy chickens (97.7%) and those afflicted with NE (94.4 %). The sequenced isolates were classified as atypical. This study did not show a direct correlation between NE occurrence and cpb2 presence. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 1 59 65 |
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English |
topic |
Clostridium perfringens necrotic enteritis chicken beta2 toxin cpb2 Iran Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Clostridium perfringens necrotic enteritis chicken beta2 toxin cpb2 Iran Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 A Tolooe B Shojadoost SM Peighambari Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens |
topic_facet |
Clostridium perfringens necrotic enteritis chicken beta2 toxin cpb2 Iran Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxins produced by some of its strains, such as beta2 toxin. The exact role of beta2 toxin in NE pathogenesis is still controversial. In the present study, C. perfringens isolates from healthy and diseased poultry flocks from different parts of Iran were analyzed by PCR assay to determine the presence of all variants of the beta2 toxin gene (cpb2). The products of two positive cpb2 PCR reactions were sequenced, compared to each other and to the cpb2 sequences published in GenBank (by multiple alignment and phylogenetic analysis). The current work represents the first study of cpb2 in poultry C. perfringens isolates in Asia, and reports the highest percentage of cpb2-positive isolates in both apparently healthy chickens (97.7%) and those afflicted with NE (94.4 %). The sequenced isolates were classified as atypical. This study did not show a direct correlation between NE occurrence and cpb2 presence. |
format |
Article in Journal/Newspaper |
author |
A Tolooe B Shojadoost SM Peighambari |
author_facet |
A Tolooe B Shojadoost SM Peighambari |
author_sort |
A Tolooe |
title |
Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens |
title_short |
Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens |
title_full |
Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens |
title_fullStr |
Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens |
title_full_unstemmed |
Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens |
title_sort |
molecular detection and characterization of cpb2 gene in clostridium perfringens isolates from healthy and diseased chickens |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000100008 https://doaj.org/article/9414ebc8d77349f0b36d721906adf1d6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 59-65 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100008 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100008 1678-9199 https://doaj.org/article/9414ebc8d77349f0b36d721906adf1d6 |
op_doi |
https://doi.org/10.1590/S1678-91992011000100008 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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17 |
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1 |
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59 |
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65 |
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1766339312033464320 |