The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic.
The infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst. The cyst wall of Entamoeba invadens (Ei), a model for Eh, is composed of chitin fibrils and three sets of chitin-binding lectins that cross-link chitin fibrils (...
Published in: | PLoS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2010
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0000750 https://doaj.org/article/940a4c3c4a314b90aa0473fe540754d1 |
id |
ftdoajarticles:oai:doaj.org/article:940a4c3c4a314b90aa0473fe540754d1 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:940a4c3c4a314b90aa0473fe540754d1 2023-05-15T15:08:11+02:00 The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. Sudip K Ghosh Katrina L Van Dellen Anirban Chatterjee Tuli Dey Rashidul Haque Phillips W Robbins John Samuelson 2010-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000750 https://doaj.org/article/940a4c3c4a314b90aa0473fe540754d1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2907411?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000750 https://doaj.org/article/940a4c3c4a314b90aa0473fe540754d1 PLoS Neglected Tropical Diseases, Vol 4, Iss 7, p e750 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000750 2022-12-31T10:45:51Z The infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst. The cyst wall of Entamoeba invadens (Ei), a model for Eh, is composed of chitin fibrils and three sets of chitin-binding lectins that cross-link chitin fibrils (multivalent Jacob lectins), self-aggregate (Jessie lectins), and remodel chitin (chitinase). The goal here was to determine how well the Ei model applies to Entamoeba cysts from humans.An Eh Jacob lectin (EhJacob2) has three predicted chitin-binding domains surrounding a large, Ser-rich spacer. Recombinant EhJacob2 made in transfected Eh trophozoites binds to particulate chitin. Sequences of PCR products using primers flanking the highly polymorphic spacer of EhJacob2 may be used to distinguish Entamoeba isolates. Antibodies to the EhJacob2, EhJessie3, and chitinase each recognize cyst walls of clinical isolates of Entamoeba. While numerous sera from patients with amebic intestinal infections and liver abscess recognize recombinant EhJacob1 and EhJessie3 lectins, few of these sera recognize recombinant EhJacob2.The EhJacob2 lectin binds chitin and is polymorphic, and Jacob2, Jessie3, and chitinase are present in cyst walls of clinical isolates of Entamoeba. These results suggest there are substantial similarities between cysts of the human pathogen (Eh) and the in vitro model (Ei), even though there are quantitative and qualitative differences in their chitin-binding lectins. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 7 e750 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sudip K Ghosh Katrina L Van Dellen Anirban Chatterjee Tuli Dey Rashidul Haque Phillips W Robbins John Samuelson The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst. The cyst wall of Entamoeba invadens (Ei), a model for Eh, is composed of chitin fibrils and three sets of chitin-binding lectins that cross-link chitin fibrils (multivalent Jacob lectins), self-aggregate (Jessie lectins), and remodel chitin (chitinase). The goal here was to determine how well the Ei model applies to Entamoeba cysts from humans.An Eh Jacob lectin (EhJacob2) has three predicted chitin-binding domains surrounding a large, Ser-rich spacer. Recombinant EhJacob2 made in transfected Eh trophozoites binds to particulate chitin. Sequences of PCR products using primers flanking the highly polymorphic spacer of EhJacob2 may be used to distinguish Entamoeba isolates. Antibodies to the EhJacob2, EhJessie3, and chitinase each recognize cyst walls of clinical isolates of Entamoeba. While numerous sera from patients with amebic intestinal infections and liver abscess recognize recombinant EhJacob1 and EhJessie3 lectins, few of these sera recognize recombinant EhJacob2.The EhJacob2 lectin binds chitin and is polymorphic, and Jacob2, Jessie3, and chitinase are present in cyst walls of clinical isolates of Entamoeba. These results suggest there are substantial similarities between cysts of the human pathogen (Eh) and the in vitro model (Ei), even though there are quantitative and qualitative differences in their chitin-binding lectins. |
format |
Article in Journal/Newspaper |
author |
Sudip K Ghosh Katrina L Van Dellen Anirban Chatterjee Tuli Dey Rashidul Haque Phillips W Robbins John Samuelson |
author_facet |
Sudip K Ghosh Katrina L Van Dellen Anirban Chatterjee Tuli Dey Rashidul Haque Phillips W Robbins John Samuelson |
author_sort |
Sudip K Ghosh |
title |
The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. |
title_short |
The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. |
title_full |
The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. |
title_fullStr |
The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. |
title_full_unstemmed |
The Jacob2 lectin of the Entamoeba histolytica cyst wall binds chitin and is polymorphic. |
title_sort |
jacob2 lectin of the entamoeba histolytica cyst wall binds chitin and is polymorphic. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000750 https://doaj.org/article/940a4c3c4a314b90aa0473fe540754d1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 7, p e750 (2010) |
op_relation |
http://europepmc.org/articles/PMC2907411?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000750 https://doaj.org/article/940a4c3c4a314b90aa0473fe540754d1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000750 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
7 |
container_start_page |
e750 |
_version_ |
1766339598527496192 |