Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature
Abstract Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine c...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:93e983d80db14756ab7b672d6e462d2d 2023-05-15T15:10:47+02:00 Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature Lídia J. Tasima Caroline Serino-Silva Daniela M. Hatakeyama Erika S. Nishiduka Alexandre K. Tashima Sávio S. Sant’Anna Kathleen F. Grego Karen de Morais-Zani Anita M. Tanaka-Azevedo 2020-04-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2019-0053 https://doaj.org/article/93e983d80db14756ab7b672d6e462d2d EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100307&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190053.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0053 https://doaj.org/article/93e983d80db14756ab7b672d6e462d2d Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) Rattlesnake Snake venom Toxins Venom variation Antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0053 2022-12-30T20:16:07Z Abstract Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy. Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and -negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected. Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Rattlesnake Snake venom Toxins Venom variation Antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Rattlesnake Snake venom Toxins Venom variation Antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Lídia J. Tasima Caroline Serino-Silva Daniela M. Hatakeyama Erika S. Nishiduka Alexandre K. Tashima Sávio S. Sant’Anna Kathleen F. Grego Karen de Morais-Zani Anita M. Tanaka-Azevedo Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
topic_facet |
Rattlesnake Snake venom Toxins Venom variation Antivenom Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy. Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and -negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected. Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom. |
format |
Article in Journal/Newspaper |
author |
Lídia J. Tasima Caroline Serino-Silva Daniela M. Hatakeyama Erika S. Nishiduka Alexandre K. Tashima Sávio S. Sant’Anna Kathleen F. Grego Karen de Morais-Zani Anita M. Tanaka-Azevedo |
author_facet |
Lídia J. Tasima Caroline Serino-Silva Daniela M. Hatakeyama Erika S. Nishiduka Alexandre K. Tashima Sávio S. Sant’Anna Kathleen F. Grego Karen de Morais-Zani Anita M. Tanaka-Azevedo |
author_sort |
Lídia J. Tasima |
title |
Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
title_short |
Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
title_full |
Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
title_fullStr |
Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
title_full_unstemmed |
Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
title_sort |
crotamine in crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature |
publisher |
SciELO |
publishDate |
2020 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0053 https://doaj.org/article/93e983d80db14756ab7b672d6e462d2d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100307&tlng=en http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190053.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0053 https://doaj.org/article/93e983d80db14756ab7b672d6e462d2d |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0053 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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