A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain

• Published in: Journal of Venomous Animals and Toxins including Tropical Diseases
• Main Authors: J. C. F. Queiroz, C. F. P. Teixeira, I. Lebrun, P. J. Spencer, T. M. A. D. Zucchi
• Format: Article in Journal/Newspaper
• Language: English
• Published: SciELO 2007
• Subjects: Aspergillus nidulans; protein purification and characterization; glycoprotein; anti-inflammatory activity; Arctic medicine. Tropical medicine; RC955-962; Toxicology. Poisons; RA1190-1270; Zoology; QL1-991; Arctic
• Online Access: https://doi.org/10.1590/S1678-91992007000200010; https://doaj.org/article/93b8a7f215ab4e6e931ad65311b2d708

Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32% glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.