The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil
Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epi...
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ftdoajarticles:oai:doaj.org/article:938390122d2842f887dc0c86d2373472 2023-05-15T15:10:42+02:00 The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil Flávia Thamiris Figueiredo Pacheco Renata Kelly Novaes Rodrigues Silva Silvia Souza de Carvalho Felipe Carvalho Rocha Gisele Maria Trindade das Chagas Daisy Chagas Gomes Hugo da Costa-Ribeiro Junior Tereza Cristina Medrado Ribeiro Ângela Peixoto de Mattos Luciano Kalabric Silva Neci Matos Soares Márcia Cristina Aquino Teixeira 2020-09-01T00:00:00Z https://doi.org/10.7705/biomedica.5161 https://doaj.org/article/938390122d2842f887dc0c86d2373472 EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5161 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5161 https://doaj.org/article/938390122d2842f887dc0c86d2373472 Biomédica: revista del Instituto Nacional de Salud, Vol 40, Iss 3, Pp 557-568 (2020) giardiasis/epidemiology child daycare centers brazil Medicine R Arctic medicine. Tropical medicine RC955-962 article 2020 ftdoajarticles https://doi.org/10.7705/biomedica.5161 2022-12-30T23:59:06Z Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil. Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil. Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin. Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years. Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 40 3 557 568 |
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English Spanish |
topic |
giardiasis/epidemiology child daycare centers brazil Medicine R Arctic medicine. Tropical medicine RC955-962 |
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giardiasis/epidemiology child daycare centers brazil Medicine R Arctic medicine. Tropical medicine RC955-962 Flávia Thamiris Figueiredo Pacheco Renata Kelly Novaes Rodrigues Silva Silvia Souza de Carvalho Felipe Carvalho Rocha Gisele Maria Trindade das Chagas Daisy Chagas Gomes Hugo da Costa-Ribeiro Junior Tereza Cristina Medrado Ribeiro Ângela Peixoto de Mattos Luciano Kalabric Silva Neci Matos Soares Márcia Cristina Aquino Teixeira The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
topic_facet |
giardiasis/epidemiology child daycare centers brazil Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction. Giardia duodenalis is an intestinal protozoan with a high prevalence in children of developing countries. Molecular studies revealed a great genetic diversity of G. duodenalis, with assemblages A and B found mainly in humans. Despite its importance, the information on the molecular epidemiology of human giardiasis is still limited in Brazil. Objective. To characterize G. duodenalis molecular isolates in children from Salvador, Bahia, Brazil. Materials and methods. Giardia duodenalis positive fecal samples were obtained from 71 children from two day care centers and 39 users of a clinical analysis laboratory. Samples were analyzed by PCR-RFLP of the glutamate dehydrogenase (gdh) and beta-giardin genes and by the sequencing of beta-giardin. Results. Of the 110 G. duodenalis samples, 80 (72.7%) amplified one or both target genes. Of these, 62 (77.5 %) were identified as assemblage A and 18 (22.5%) as assemblage B. The subassemblage AII was identified in 58.8% (n=47) of isolates followed by the sub-assemblage AI (18.8%, n=15), BIV (11.2%, n=9), and BIII (5.0%, n=4). The AII sub-assemblage was the most frequent in children of both day care centers whereas AI was found only in the group attended at the clinical laboratory. Sub-assemblage AII predominated in children under two years. Conclusions. The higher frequency of AII sub-assemblage suggests that anthroponotic transmission is more common in Salvador, but that zoonotic transmission pathways are also present and a change in susceptibility to different molecular patterns of Giardia may occur during child growth. |
format |
Article in Journal/Newspaper |
author |
Flávia Thamiris Figueiredo Pacheco Renata Kelly Novaes Rodrigues Silva Silvia Souza de Carvalho Felipe Carvalho Rocha Gisele Maria Trindade das Chagas Daisy Chagas Gomes Hugo da Costa-Ribeiro Junior Tereza Cristina Medrado Ribeiro Ângela Peixoto de Mattos Luciano Kalabric Silva Neci Matos Soares Márcia Cristina Aquino Teixeira |
author_facet |
Flávia Thamiris Figueiredo Pacheco Renata Kelly Novaes Rodrigues Silva Silvia Souza de Carvalho Felipe Carvalho Rocha Gisele Maria Trindade das Chagas Daisy Chagas Gomes Hugo da Costa-Ribeiro Junior Tereza Cristina Medrado Ribeiro Ângela Peixoto de Mattos Luciano Kalabric Silva Neci Matos Soares Márcia Cristina Aquino Teixeira |
author_sort |
Flávia Thamiris Figueiredo Pacheco |
title |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_short |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_full |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_fullStr |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_full_unstemmed |
The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil |
title_sort |
predominance of giardia duodenalis aii sub-assemblage in young children from salvador, bahia, brazil |
publisher |
Instituto Nacional de Salud |
publishDate |
2020 |
url |
https://doi.org/10.7705/biomedica.5161 https://doaj.org/article/938390122d2842f887dc0c86d2373472 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 40, Iss 3, Pp 557-568 (2020) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/5161 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5161 https://doaj.org/article/938390122d2842f887dc0c86d2373472 |
op_doi |
https://doi.org/10.7705/biomedica.5161 |
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