Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga
An immunoprecipitation technique, ELIEDA (enzyme-linked-immuno-electro-diffusion assay), was evaluated for the diagnosis of Schistosoma mansoni infection with low worm burden. One hundred of serum samples from patients excreting less than 600 eggs per gram of feces (epg), with unrelated diseases and...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
1995
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ftdoajarticles:oai:doaj.org/article:932865b555b74d14800b9e65e599028a 2024-09-09T19:28:29+00:00 Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga Grace Mary Leal-Bacelar Hermínia Y. Kanamura Sumie Hoshino-Shimizu Dirce Mary C. Lima Clarice P. Abrantes-Lemos Luiz Caetano da Silva 1995-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651995000200006 https://doaj.org/article/932865b555b74d14800b9e65e599028a EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000200006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000200006 0036-4665 1678-9946 https://doaj.org/article/932865b555b74d14800b9e65e599028a Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 2, Pp 123-127 (1995) Schistosoma mansoni Immunodiagnosis ELIEDA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1995 ftdoajarticles https://doi.org/10.1590/S0036-46651995000200006 2024-08-05T17:49:30Z An immunoprecipitation technique, ELIEDA (enzyme-linked-immuno-electro-diffusion assay), was evaluated for the diagnosis of Schistosoma mansoni infection with low worm burden. One hundred of serum samples from patients excreting less than 600 eggs per gram of feces (epg), with unrelated diseases and clinically healthy subjects were studied. In patients with egg counts higher than 200 epg, the sensitivities of IgM and IgG ELIEDA were 1.000 and 0.923, respectively, not differing from other Serologic techniques, such as indirect hemaglutination (IHAT), immunofluorescence (IFT) tests and immuno-electrodiffusion assay (IEDA). However in patients with low egg counts (< 100 epg), the IgG ELIEDA provided better results (0.821) than IgM ELIEDA (0.679), showing sensitivity that did not differ from that of IgG IFT (0.929), but lower than that of IgM IFT (0.964). However, its sensivity was higher than that found with IHAT (0.607) and IEDA (0.536). The specificity of IgG ELIEDA was comparable to that of other techniques. The data indicate that IgG ELIEDA might be useful for the diagnosis of slight S. mansoni infections, and the cellulose acetate membrane strips can be stored for further retrospective studies. A técnica de imunoprecipitação ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) foi avaliada para fins diagnósticos da esquistossomose mansoni em pacientes com baixa carga parasitária. Amostras de soros de 50 pacientes com exame de fezes positivo para S. mansoni (carga parasitária < 600 ovos por grama de fezes = opg) e 50 não esquistossomóticos (30 com outras afecções e 20 normais) foram estudadas. Em pacientes com carga parasitária acima de 200 opg, a sensibilidade da técnica de ELIEDA, tanto para anticorpos IgG como IgM, respectivamente 1,000 e 0,923, não diferiu da observada para outras reações sorológicas, como a de hemaglutinação (RHA), imunofluorescênca (RIF) e imunoeletrodifusão (IED). Entretanto, naqueles com baixa carga (< 100 opg), a ELIEDA-IgG mostrou resultados mais satisfatórios (0,821) que a ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rif ENVELOPE(-16.172,-16.172,66.526,66.526) Revista do Instituto de Medicina Tropical de São Paulo 37 2 123 127 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Schistosoma mansoni Immunodiagnosis ELIEDA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Schistosoma mansoni Immunodiagnosis ELIEDA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Grace Mary Leal-Bacelar Hermínia Y. Kanamura Sumie Hoshino-Shimizu Dirce Mary C. Lima Clarice P. Abrantes-Lemos Luiz Caetano da Silva Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga |
topic_facet |
Schistosoma mansoni Immunodiagnosis ELIEDA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
An immunoprecipitation technique, ELIEDA (enzyme-linked-immuno-electro-diffusion assay), was evaluated for the diagnosis of Schistosoma mansoni infection with low worm burden. One hundred of serum samples from patients excreting less than 600 eggs per gram of feces (epg), with unrelated diseases and clinically healthy subjects were studied. In patients with egg counts higher than 200 epg, the sensitivities of IgM and IgG ELIEDA were 1.000 and 0.923, respectively, not differing from other Serologic techniques, such as indirect hemaglutination (IHAT), immunofluorescence (IFT) tests and immuno-electrodiffusion assay (IEDA). However in patients with low egg counts (< 100 epg), the IgG ELIEDA provided better results (0.821) than IgM ELIEDA (0.679), showing sensitivity that did not differ from that of IgG IFT (0.929), but lower than that of IgM IFT (0.964). However, its sensivity was higher than that found with IHAT (0.607) and IEDA (0.536). The specificity of IgG ELIEDA was comparable to that of other techniques. The data indicate that IgG ELIEDA might be useful for the diagnosis of slight S. mansoni infections, and the cellulose acetate membrane strips can be stored for further retrospective studies. A técnica de imunoprecipitação ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) foi avaliada para fins diagnósticos da esquistossomose mansoni em pacientes com baixa carga parasitária. Amostras de soros de 50 pacientes com exame de fezes positivo para S. mansoni (carga parasitária < 600 ovos por grama de fezes = opg) e 50 não esquistossomóticos (30 com outras afecções e 20 normais) foram estudadas. Em pacientes com carga parasitária acima de 200 opg, a sensibilidade da técnica de ELIEDA, tanto para anticorpos IgG como IgM, respectivamente 1,000 e 0,923, não diferiu da observada para outras reações sorológicas, como a de hemaglutinação (RHA), imunofluorescênca (RIF) e imunoeletrodifusão (IED). Entretanto, naqueles com baixa carga (< 100 opg), a ELIEDA-IgG mostrou resultados mais satisfatórios (0,821) que a ... |
format |
Article in Journal/Newspaper |
author |
Grace Mary Leal-Bacelar Hermínia Y. Kanamura Sumie Hoshino-Shimizu Dirce Mary C. Lima Clarice P. Abrantes-Lemos Luiz Caetano da Silva |
author_facet |
Grace Mary Leal-Bacelar Hermínia Y. Kanamura Sumie Hoshino-Shimizu Dirce Mary C. Lima Clarice P. Abrantes-Lemos Luiz Caetano da Silva |
author_sort |
Grace Mary Leal-Bacelar |
title |
Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga |
title_short |
Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga |
title_full |
Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga |
title_fullStr |
Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga |
title_full_unstemmed |
Evaluation of the enzyme-linked-immuno-electro-diffusion-assay (ELIEDA) for the diagnosis of Schistosoma mansoni infection with low worm burden Avaliação da técnica de ELIEDA (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo Schistosoma mansoni de baixa carga |
title_sort |
evaluation of the enzyme-linked-immuno-electro-diffusion-assay (elieda) for the diagnosis of schistosoma mansoni infection with low worm burden avaliação da técnica de elieda (enzyme-linked-immuno-electro-diffusion-assay) para o diagnóstico da infecção pelo schistosoma mansoni de baixa carga |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1995 |
url |
https://doi.org/10.1590/S0036-46651995000200006 https://doaj.org/article/932865b555b74d14800b9e65e599028a |
long_lat |
ENVELOPE(-16.172,-16.172,66.526,66.526) |
geographic |
Arctic Rif |
geographic_facet |
Arctic Rif |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 2, Pp 123-127 (1995) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000200006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000200006 0036-4665 1678-9946 https://doaj.org/article/932865b555b74d14800b9e65e599028a |
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https://doi.org/10.1590/S0036-46651995000200006 |
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