Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo
Objective: To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in vivo. Methods: The effects of the methanolic extract of Ephedra alata on the viability, migration as well as apoptosis of breast cancer 4T1 cells were measured usi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2024
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ftdoajarticles:oai:doaj.org/article:9319624434314e8aa20ba6ef35604de9 2024-09-09T19:25:47+00:00 Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo Sioud Fairouz Lahmer Aida Selmi Mouna Chaabane Fadwa Chekir-Ghedira Leila 2024-04-01T00:00:00Z https://doi.org/10.4103/apjtb.apjtb_2_24 https://doaj.org/article/9319624434314e8aa20ba6ef35604de9 EN eng Wolters Kluwer Medknow Publications https://journals.lww.com/10.4103/apjtb.apjtb_2_24 https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/apjtb.apjtb_2_24 https://doaj.org/article/9319624434314e8aa20ba6ef35604de9 Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 4, Pp 154-161 (2024) ephedra breast cancer apoptosis antioxidant migration Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2024 ftdoajarticles https://doi.org/10.4103/apjtb.apjtb_2_24 2024-08-05T17:49:09Z Objective: To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in vivo. Methods: The effects of the methanolic extract of Ephedra alata on the viability, migration as well as apoptosis of breast cancer 4T1 cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay, and annexin V-FITC staining assay, respectively. Histological examination was also carried out. Moreover, a murine breast cancer model was established to evaluate the inhibitory effect of the extract. Biochemical parameters including hepatic and non-hepatic enzymes, malondialdehyde, and glutathione were investigated. Results: The methanolic extract of Ephedra alata showed a strong anti-proliferative and anti-migratory activity against 4T1 cells in a dose-dependent manner. It also induced apoptosis in 4T1 cells. In an in vivo mouse model, the extract markedly inhibited tumor growth, reduced malondialdehyde, and hepatic and non-hepatic enzymes as well as increased glutathione level. Conclusions: The methanolic extract of Ephedra alata inhibits breast cancer in vitro and in vivo, which may be a promising anticancer agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 14 4 154 161 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
ephedra breast cancer apoptosis antioxidant migration Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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ephedra breast cancer apoptosis antioxidant migration Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sioud Fairouz Lahmer Aida Selmi Mouna Chaabane Fadwa Chekir-Ghedira Leila Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo |
topic_facet |
ephedra breast cancer apoptosis antioxidant migration Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in vivo. Methods: The effects of the methanolic extract of Ephedra alata on the viability, migration as well as apoptosis of breast cancer 4T1 cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, Transwell assay, and annexin V-FITC staining assay, respectively. Histological examination was also carried out. Moreover, a murine breast cancer model was established to evaluate the inhibitory effect of the extract. Biochemical parameters including hepatic and non-hepatic enzymes, malondialdehyde, and glutathione were investigated. Results: The methanolic extract of Ephedra alata showed a strong anti-proliferative and anti-migratory activity against 4T1 cells in a dose-dependent manner. It also induced apoptosis in 4T1 cells. In an in vivo mouse model, the extract markedly inhibited tumor growth, reduced malondialdehyde, and hepatic and non-hepatic enzymes as well as increased glutathione level. Conclusions: The methanolic extract of Ephedra alata inhibits breast cancer in vitro and in vivo, which may be a promising anticancer agent. |
format |
Article in Journal/Newspaper |
author |
Sioud Fairouz Lahmer Aida Selmi Mouna Chaabane Fadwa Chekir-Ghedira Leila |
author_facet |
Sioud Fairouz Lahmer Aida Selmi Mouna Chaabane Fadwa Chekir-Ghedira Leila |
author_sort |
Sioud Fairouz |
title |
Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo |
title_short |
Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo |
title_full |
Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo |
title_fullStr |
Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo |
title_full_unstemmed |
Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo |
title_sort |
methanolic extract of ephedra alata inhibits breast cancer cells in vitro and in vivo |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2024 |
url |
https://doi.org/10.4103/apjtb.apjtb_2_24 https://doaj.org/article/9319624434314e8aa20ba6ef35604de9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 14, Iss 4, Pp 154-161 (2024) |
op_relation |
https://journals.lww.com/10.4103/apjtb.apjtb_2_24 https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/apjtb.apjtb_2_24 https://doaj.org/article/9319624434314e8aa20ba6ef35604de9 |
op_doi |
https://doi.org/10.4103/apjtb.apjtb_2_24 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
14 |
container_issue |
4 |
container_start_page |
154 |
op_container_end_page |
161 |
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1809895508376813568 |