Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection.
Congenital infection of Trypanosoma cruzi allows transmission of this parasite through generations. Despite the problematic that this entails, little is known about the placenta environment genetic response produced against infection. We performed functional genomics by microarray analysis in C57Bl/...
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ftdoajarticles:oai:doaj.org/article:93006ca35c8541dfbedcfb78bd366b53 2023-05-15T15:11:24+02:00 Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. Natalia Anahí Juiz María Elisa Solana Gonzalo Raúl Acevedo Alejandro Francisco Benatar Juan Carlos Ramirez Priscilla Almeida da Costa Andrea Mara Macedo Silvia Andrea Longhi Alejandro G Schijman 2017-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005436 https://doaj.org/article/93006ca35c8541dfbedcfb78bd366b53 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5358786?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005436 https://doaj.org/article/93006ca35c8541dfbedcfb78bd366b53 PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005436 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005436 2022-12-31T15:54:44Z Congenital infection of Trypanosoma cruzi allows transmission of this parasite through generations. Despite the problematic that this entails, little is known about the placenta environment genetic response produced against infection. We performed functional genomics by microarray analysis in C57Bl/6J mice comparing placentas from uninfected animals and from animals infected with two different T. cruzi strains: K98, a clone of the non-lethal myotropic CA-I strain (TcI), and VD (TcVI), isolated from a human case of congenital infection. Analysis of networks by GeneMANIA of differentially expressed genes showed that "Secretory Granule" was a pathway down-regulated in both infected groups, whereas "Innate Immune Response" and "Response to Interferon-gamma" were pathways up-regulated in VD infection but not in K98. Applying another approach, the GSEA algorithm that detects small changes in predetermined gene sets, we found that metabolic processes, transcription and macromolecular transport were down-regulated in infected placentas environment and some pathways related to cascade signaling had opposite regulation: over-represented in VD and down-regulated in K98 group. We also have found a stronger tropism to the placental organ by VD strain, by detection of parasite DNA and RNA, suggesting living parasites. Our study is the first one to describe in a murine model the genetic response of placental environment to T. cruzi infection and suggests the development of a strong immune response, parasite genotype-dependent, to the detriment of cellular metabolism, which may contribute to control infection preventing the risk of congenital transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 3 e0005436 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Natalia Anahí Juiz María Elisa Solana Gonzalo Raúl Acevedo Alejandro Francisco Benatar Juan Carlos Ramirez Priscilla Almeida da Costa Andrea Mara Macedo Silvia Andrea Longhi Alejandro G Schijman Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Congenital infection of Trypanosoma cruzi allows transmission of this parasite through generations. Despite the problematic that this entails, little is known about the placenta environment genetic response produced against infection. We performed functional genomics by microarray analysis in C57Bl/6J mice comparing placentas from uninfected animals and from animals infected with two different T. cruzi strains: K98, a clone of the non-lethal myotropic CA-I strain (TcI), and VD (TcVI), isolated from a human case of congenital infection. Analysis of networks by GeneMANIA of differentially expressed genes showed that "Secretory Granule" was a pathway down-regulated in both infected groups, whereas "Innate Immune Response" and "Response to Interferon-gamma" were pathways up-regulated in VD infection but not in K98. Applying another approach, the GSEA algorithm that detects small changes in predetermined gene sets, we found that metabolic processes, transcription and macromolecular transport were down-regulated in infected placentas environment and some pathways related to cascade signaling had opposite regulation: over-represented in VD and down-regulated in K98 group. We also have found a stronger tropism to the placental organ by VD strain, by detection of parasite DNA and RNA, suggesting living parasites. Our study is the first one to describe in a murine model the genetic response of placental environment to T. cruzi infection and suggests the development of a strong immune response, parasite genotype-dependent, to the detriment of cellular metabolism, which may contribute to control infection preventing the risk of congenital transmission. |
format |
Article in Journal/Newspaper |
author |
Natalia Anahí Juiz María Elisa Solana Gonzalo Raúl Acevedo Alejandro Francisco Benatar Juan Carlos Ramirez Priscilla Almeida da Costa Andrea Mara Macedo Silvia Andrea Longhi Alejandro G Schijman |
author_facet |
Natalia Anahí Juiz María Elisa Solana Gonzalo Raúl Acevedo Alejandro Francisco Benatar Juan Carlos Ramirez Priscilla Almeida da Costa Andrea Mara Macedo Silvia Andrea Longhi Alejandro G Schijman |
author_sort |
Natalia Anahí Juiz |
title |
Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
title_short |
Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
title_full |
Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
title_fullStr |
Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
title_full_unstemmed |
Different genotypes of Trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
title_sort |
different genotypes of trypanosoma cruzi produce distinctive placental environment genetic response in chronic experimental infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005436 https://doaj.org/article/93006ca35c8541dfbedcfb78bd366b53 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005436 (2017) |
op_relation |
http://europepmc.org/articles/PMC5358786?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005436 https://doaj.org/article/93006ca35c8541dfbedcfb78bd366b53 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005436 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
3 |
container_start_page |
e0005436 |
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1766342258588647424 |