Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress
Oxidative stress is involved in the pathophysiology of many neurodegenerative diseases. Lichens have antioxidant properties attributed to their own secondary metabolites with phenol groups. Very few studies delve into the protective capacity of lichens based on their antioxidant properties and their...
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ftdoajarticles:oai:doaj.org/article:92f6f1c0832340ff9c2424fff06a51fe 2023-05-15T15:25:25+02:00 Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress Isabel Ureña-Vacas Elena González-Burgos Pradeep Kumar Divakar María Pilar Gómez-Serranillos 2022-10-01T00:00:00Z https://doi.org/10.3390/molecules27196520 https://doaj.org/article/92f6f1c0832340ff9c2424fff06a51fe EN eng MDPI AG https://www.mdpi.com/1420-3049/27/19/6520 https://doaj.org/toc/1420-3049 doi:10.3390/molecules27196520 1420-3049 https://doaj.org/article/92f6f1c0832340ff9c2424fff06a51fe Molecules, Vol 27, Iss 6520, p 6520 (2022) lichens neuroprotection cetrarioid clade oxidative stress Organic chemistry QD241-441 article 2022 ftdoajarticles https://doi.org/10.3390/molecules27196520 2022-12-30T19:46:32Z Oxidative stress is involved in the pathophysiology of many neurodegenerative diseases. Lichens have antioxidant properties attributed to their own secondary metabolites with phenol groups. Very few studies delve into the protective capacity of lichens based on their antioxidant properties and their action mechanism. The present study evaluates the neuroprotective role of Dactylina arctica , Nephromopsis stracheyi , Tuckermannopsis americana and Vulpicida pinastri methanol extracts in a hydrogen peroxide (H 2 O 2 ) oxidative stress model in neuroblastoma cell line “SH-SY5Y cells”. Cells were pretreated with different concentrations of lichen extracts (24 h) before H 2 O 2 (250 µM, 1 h). Our results showed that D. arctica (10 µg/mL), N. stracheyi (25 µg/mL), T. americana (50 µg/mL) and V. pinastri (5 µg/mL) prevented cell death and morphological changes. Moreover, these lichens significantly inhibited reactive oxygen species (ROS) production and lipid peroxidation and increased superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) levels. Furthermore, they attenuated mitochondrial membrane potential decline and calcium homeostasis disruption. Finally, high-performance liquid chromatography (HPLC) analysis revealed that the secondary metabolites were gyrophoric acid and lecanoric acid in D. artica , usnic acid, pinastric acid and vulpinic acid in V. pinastri , and alectoronic acid in T. americana . In conclusion, D. arctica and V. pinastri are the most promising lichens to prevent and to treat oxidative stress-related neurodegenerative diseases. Article in Journal/Newspaper artica Directory of Open Access Journals: DOAJ Articles Molecules 27 19 6520 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
lichens neuroprotection cetrarioid clade oxidative stress Organic chemistry QD241-441 |
spellingShingle |
lichens neuroprotection cetrarioid clade oxidative stress Organic chemistry QD241-441 Isabel Ureña-Vacas Elena González-Burgos Pradeep Kumar Divakar María Pilar Gómez-Serranillos Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress |
topic_facet |
lichens neuroprotection cetrarioid clade oxidative stress Organic chemistry QD241-441 |
description |
Oxidative stress is involved in the pathophysiology of many neurodegenerative diseases. Lichens have antioxidant properties attributed to their own secondary metabolites with phenol groups. Very few studies delve into the protective capacity of lichens based on their antioxidant properties and their action mechanism. The present study evaluates the neuroprotective role of Dactylina arctica , Nephromopsis stracheyi , Tuckermannopsis americana and Vulpicida pinastri methanol extracts in a hydrogen peroxide (H 2 O 2 ) oxidative stress model in neuroblastoma cell line “SH-SY5Y cells”. Cells were pretreated with different concentrations of lichen extracts (24 h) before H 2 O 2 (250 µM, 1 h). Our results showed that D. arctica (10 µg/mL), N. stracheyi (25 µg/mL), T. americana (50 µg/mL) and V. pinastri (5 µg/mL) prevented cell death and morphological changes. Moreover, these lichens significantly inhibited reactive oxygen species (ROS) production and lipid peroxidation and increased superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) levels. Furthermore, they attenuated mitochondrial membrane potential decline and calcium homeostasis disruption. Finally, high-performance liquid chromatography (HPLC) analysis revealed that the secondary metabolites were gyrophoric acid and lecanoric acid in D. artica , usnic acid, pinastric acid and vulpinic acid in V. pinastri , and alectoronic acid in T. americana . In conclusion, D. arctica and V. pinastri are the most promising lichens to prevent and to treat oxidative stress-related neurodegenerative diseases. |
format |
Article in Journal/Newspaper |
author |
Isabel Ureña-Vacas Elena González-Burgos Pradeep Kumar Divakar María Pilar Gómez-Serranillos |
author_facet |
Isabel Ureña-Vacas Elena González-Burgos Pradeep Kumar Divakar María Pilar Gómez-Serranillos |
author_sort |
Isabel Ureña-Vacas |
title |
Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress |
title_short |
Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress |
title_full |
Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress |
title_fullStr |
Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress |
title_full_unstemmed |
Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress |
title_sort |
lichen extracts from cetrarioid clade provide neuroprotection against hydrogen peroxide-induced oxidative stress |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/molecules27196520 https://doaj.org/article/92f6f1c0832340ff9c2424fff06a51fe |
genre |
artica |
genre_facet |
artica |
op_source |
Molecules, Vol 27, Iss 6520, p 6520 (2022) |
op_relation |
https://www.mdpi.com/1420-3049/27/19/6520 https://doaj.org/toc/1420-3049 doi:10.3390/molecules27196520 1420-3049 https://doaj.org/article/92f6f1c0832340ff9c2424fff06a51fe |
op_doi |
https://doi.org/10.3390/molecules27196520 |
container_title |
Molecules |
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27 |
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19 |
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6520 |
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1766356087299112960 |