Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection.
Chikungunya virus (CHIKV) is a human pathogen causing outbreaks of febrile illness for which vaccines and specific treatments remain unavailable. Autophagy-related (ATG) proteins and autophagy receptors are a set of host factors that participate in autophagy, but have also shown to function in other...
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ftdoajarticles:oai:doaj.org/article:92e78b227bcb424ebd2bcf12ac910d93 2024-01-28T10:03:57+01:00 Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. Liliana Echavarria-Consuegra Nilima Dinesh Kumar Marleen van der Laan Mario Mauthe Denise Van de Pol Fulvio Reggiori Jolanda M Smit 2023-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010751 https://doaj.org/article/92e78b227bcb424ebd2bcf12ac910d93 EN eng Public Library of Science (PLoS) https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010751&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010751 https://doaj.org/article/92e78b227bcb424ebd2bcf12ac910d93 PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0010751 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010751 2023-12-31T01:49:22Z Chikungunya virus (CHIKV) is a human pathogen causing outbreaks of febrile illness for which vaccines and specific treatments remain unavailable. Autophagy-related (ATG) proteins and autophagy receptors are a set of host factors that participate in autophagy, but have also shown to function in other unrelated cellular pathways. Although autophagy is reported to both inhibit and enhance CHIKV replication, the specific role of individual ATG proteins remains largely unknown. Here, a siRNA screen was performed to evaluate the importance of the ATG proteome and autophagy receptors in controlling CHIKV infection. We observed that 7 out of 50 ATG proteins impact the replication of CHIKV. Among those, depletion of the mitochondrial protein and autophagy receptor BCL2 Interacting Protein 3 (BNIP3) increased CHIKV infection. Interestingly, BNIP3 controls CHIKV independently of autophagy and cell death. Detailed analysis of the CHIKV viral cycle revealed that BNIP3 interferes with the early stages of infection. Moreover, the antiviral role of BNIP3 was found conserved across two distinct CHIKV genotypes and the closely related Semliki Forest virus. Altogether, this study describes a novel and previously unknown function of the mitochondrial protein BNIP3 in the control of the early stages of the alphavirus viral cycle. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 11 e0010751 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Liliana Echavarria-Consuegra Nilima Dinesh Kumar Marleen van der Laan Mario Mauthe Denise Van de Pol Fulvio Reggiori Jolanda M Smit Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chikungunya virus (CHIKV) is a human pathogen causing outbreaks of febrile illness for which vaccines and specific treatments remain unavailable. Autophagy-related (ATG) proteins and autophagy receptors are a set of host factors that participate in autophagy, but have also shown to function in other unrelated cellular pathways. Although autophagy is reported to both inhibit and enhance CHIKV replication, the specific role of individual ATG proteins remains largely unknown. Here, a siRNA screen was performed to evaluate the importance of the ATG proteome and autophagy receptors in controlling CHIKV infection. We observed that 7 out of 50 ATG proteins impact the replication of CHIKV. Among those, depletion of the mitochondrial protein and autophagy receptor BCL2 Interacting Protein 3 (BNIP3) increased CHIKV infection. Interestingly, BNIP3 controls CHIKV independently of autophagy and cell death. Detailed analysis of the CHIKV viral cycle revealed that BNIP3 interferes with the early stages of infection. Moreover, the antiviral role of BNIP3 was found conserved across two distinct CHIKV genotypes and the closely related Semliki Forest virus. Altogether, this study describes a novel and previously unknown function of the mitochondrial protein BNIP3 in the control of the early stages of the alphavirus viral cycle. |
format |
Article in Journal/Newspaper |
author |
Liliana Echavarria-Consuegra Nilima Dinesh Kumar Marleen van der Laan Mario Mauthe Denise Van de Pol Fulvio Reggiori Jolanda M Smit |
author_facet |
Liliana Echavarria-Consuegra Nilima Dinesh Kumar Marleen van der Laan Mario Mauthe Denise Van de Pol Fulvio Reggiori Jolanda M Smit |
author_sort |
Liliana Echavarria-Consuegra |
title |
Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. |
title_short |
Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. |
title_full |
Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. |
title_fullStr |
Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. |
title_full_unstemmed |
Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. |
title_sort |
mitochondrial protein bnip3 regulates chikungunya virus replication in the early stages of infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0010751 https://doaj.org/article/92e78b227bcb424ebd2bcf12ac910d93 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 11, p e0010751 (2023) |
op_relation |
https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0010751&type=printable https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010751 https://doaj.org/article/92e78b227bcb424ebd2bcf12ac910d93 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010751 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
11 |
container_start_page |
e0010751 |
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1789329529594970112 |