Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy
Objective: To assess normal conjunctival cytological and bacteriological/fungal flora features in the Mediterranean buffalo (Bubalus bubalis). Methods: Swabs were taken from the inferior conjunctival sac of both eyes of 57 healthy female buffaloes aged 24–36 months, with no evidence of ocular diseas...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2015
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Online Access: | https://doi.org/10.1016/j.apjtb.2015.06.017 https://doaj.org/article/92c0a10e1e3e425a9df5e551c35fe828 |
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ftdoajarticles:oai:doaj.org/article:92c0a10e1e3e425a9df5e551c35fe828 2023-05-15T15:13:32+02:00 Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy Barbara Lamagna Maria Pia Pasolini Sandra Nizza Karina Mallardo Maurizio Formicola Alessandro Costagliola Gerardo Fatone Filomena Fiorito Orlando Paciello Luisa De Martino 2015-11-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.06.017 https://doaj.org/article/92c0a10e1e3e425a9df5e551c35fe828 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115001847 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.06.017 https://doaj.org/article/92c0a10e1e3e425a9df5e551c35fe828 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 11, Pp 889-895 (2015) Mediterranean buffalo Conjunctival cytological examination Bacteriologic culture Antimicrobial resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.06.017 2022-12-30T22:16:04Z Objective: To assess normal conjunctival cytological and bacteriological/fungal flora features in the Mediterranean buffalo (Bubalus bubalis). Methods: Swabs were taken from the inferior conjunctival sac of both eyes of 57 healthy female buffaloes aged 24–36 months, with no evidence of ocular disease, farmed in Campania region (Southern Italy), for microbiological analysis. Conjunctival eye specimens of both eyes were subsequently obtained by a cyto-brush, for cytological analysis. The antimicrobial susceptibility of bacterial isolates was also determined using the disk-diffusion method on Mueller Hinton agar plates. Results: Cytological examination of conjunctival swab specimens (114 eyes) revealed epithelial cells (basal, intermediate, columnar and superficial) in all samples, whereas neutrophils, lymphocytes and plasma cells were present in 70%, 10% and 2% of samples, respectively. Microorganisms, for a total of 261 aerobic bacteria and 6 fungi, were isolated from 112/114 conjunctival samples [98.25%; 95% confidence interval (CI): 93.18–99.70]. Only two conjunctival swabs did not yield bacteria and/or fungi (2/114, 1.75%; 95% CI: 0.30–6.82). Gram-positive aerobes were most commonly cultured (181/261, 69.35%; 95% CI: 63.31–74.81), with Enterococcus faecium and Staphylococcus lentus predominating. Escherichia coli was the most frequently isolated as Gram-negative bacteria (80/261, 30.65%; 95% CI: 25.19–36.69). The antimicrobial resistance patterns of the isolated bacteria showed amoxycillin/clavulanic acid and cephalothin as the least sensitive antibiotics for both Gram-positive and Gram-negative bacteria. Conclusions: These results provided first information on normal conjunctival ocular microflora and cytological features in Mediterranean buffalo. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Mueller ENVELOPE(55.533,55.533,-66.917,-66.917) Asian Pacific Journal of Tropical Biomedicine 5 11 889 895 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Mediterranean buffalo Conjunctival cytological examination Bacteriologic culture Antimicrobial resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Mediterranean buffalo Conjunctival cytological examination Bacteriologic culture Antimicrobial resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Barbara Lamagna Maria Pia Pasolini Sandra Nizza Karina Mallardo Maurizio Formicola Alessandro Costagliola Gerardo Fatone Filomena Fiorito Orlando Paciello Luisa De Martino Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy |
topic_facet |
Mediterranean buffalo Conjunctival cytological examination Bacteriologic culture Antimicrobial resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To assess normal conjunctival cytological and bacteriological/fungal flora features in the Mediterranean buffalo (Bubalus bubalis). Methods: Swabs were taken from the inferior conjunctival sac of both eyes of 57 healthy female buffaloes aged 24–36 months, with no evidence of ocular disease, farmed in Campania region (Southern Italy), for microbiological analysis. Conjunctival eye specimens of both eyes were subsequently obtained by a cyto-brush, for cytological analysis. The antimicrobial susceptibility of bacterial isolates was also determined using the disk-diffusion method on Mueller Hinton agar plates. Results: Cytological examination of conjunctival swab specimens (114 eyes) revealed epithelial cells (basal, intermediate, columnar and superficial) in all samples, whereas neutrophils, lymphocytes and plasma cells were present in 70%, 10% and 2% of samples, respectively. Microorganisms, for a total of 261 aerobic bacteria and 6 fungi, were isolated from 112/114 conjunctival samples [98.25%; 95% confidence interval (CI): 93.18–99.70]. Only two conjunctival swabs did not yield bacteria and/or fungi (2/114, 1.75%; 95% CI: 0.30–6.82). Gram-positive aerobes were most commonly cultured (181/261, 69.35%; 95% CI: 63.31–74.81), with Enterococcus faecium and Staphylococcus lentus predominating. Escherichia coli was the most frequently isolated as Gram-negative bacteria (80/261, 30.65%; 95% CI: 25.19–36.69). The antimicrobial resistance patterns of the isolated bacteria showed amoxycillin/clavulanic acid and cephalothin as the least sensitive antibiotics for both Gram-positive and Gram-negative bacteria. Conclusions: These results provided first information on normal conjunctival ocular microflora and cytological features in Mediterranean buffalo. |
format |
Article in Journal/Newspaper |
author |
Barbara Lamagna Maria Pia Pasolini Sandra Nizza Karina Mallardo Maurizio Formicola Alessandro Costagliola Gerardo Fatone Filomena Fiorito Orlando Paciello Luisa De Martino |
author_facet |
Barbara Lamagna Maria Pia Pasolini Sandra Nizza Karina Mallardo Maurizio Formicola Alessandro Costagliola Gerardo Fatone Filomena Fiorito Orlando Paciello Luisa De Martino |
author_sort |
Barbara Lamagna |
title |
Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy |
title_short |
Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy |
title_full |
Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy |
title_fullStr |
Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy |
title_full_unstemmed |
Conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy Mediterranean buffaloes (Bubalus bubalis) from Southern Italy |
title_sort |
conjunctival cytological examination, bacteriological culture, and antimicrobial resistance profiles of healthy mediterranean buffaloes (bubalus bubalis) from southern italy |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.06.017 https://doaj.org/article/92c0a10e1e3e425a9df5e551c35fe828 |
long_lat |
ENVELOPE(55.533,55.533,-66.917,-66.917) |
geographic |
Arctic Mueller |
geographic_facet |
Arctic Mueller |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 11, Pp 889-895 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115001847 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.06.017 https://doaj.org/article/92c0a10e1e3e425a9df5e551c35fe828 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.06.017 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
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5 |
container_issue |
11 |
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889 |
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895 |
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