A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET".
Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African cou...
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ftdoajarticles:oai:doaj.org/article:929f63fe7c044fe687ba7eb80d26f10a 2023-05-15T15:12:41+02:00 A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". Estelle Marion Numfor Hycenth Sundeep Chaitanya Vedithi Marie Robbe-Saule Valérie Donkeng Line-Marlène Ganlonon Affolabi Dissou Solange Kakou Ngazoa Marie-Jose Kabedi Arsène Mabika Mabika Richard Phillips Michael Frimpong Dorothy Yeboah-Manu Vera Yatta Walker Olaoluwa Akinwale Maman Issaka Gisela Bretzel Kingsley Asiedu Sara Eyangoh 2022-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010908 https://doaj.org/article/929f63fe7c044fe687ba7eb80d26f10a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010908 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010908 https://doaj.org/article/929f63fe7c044fe687ba7eb80d26f10a PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010908 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010908 2022-12-30T20:06:09Z Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African countries, South America and Western Pacific regions. Majority of cases are spread across West and Central Africa. The mode of transmission is unclear, hindering the implementation of adequate prevention for the population. Currently, early diagnosis and treatment are crucial to minimizing morbidity, costs and preventing long-term disability. Biological confirmation of clinical diagnosis of Buruli ulcer is essential before starting chemotherapy. Indeed, differential diagnosis are numerous and Buruli ulcer has varying clinical presentations. Up to now, the gold standard biological confirmation is the quantitative PCR, targeting the insertion sequence IS2404 of M. ulcerans performed on cutaneous samples. Due to the low PCR confirmation rate in endemic African countries (under 30% in 2018) for numerous identified reasons within this article, 11 laboratories decided to combine their efforts to create the network "BU-LABNET" in 2019. The first step of the network was to harmonize the procedures and ship specific reagents to each laboratory. With this system in place, implementation of these procedures for testing and follow-up was easy and the laboratories were able to carry out their first quality control with a very high success rate. It is now time to integrate other neglected tropical diseases to this platform, such as yaws or leprosy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Pacific PLOS Neglected Tropical Diseases 16 11 e0010908 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Estelle Marion Numfor Hycenth Sundeep Chaitanya Vedithi Marie Robbe-Saule Valérie Donkeng Line-Marlène Ganlonon Affolabi Dissou Solange Kakou Ngazoa Marie-Jose Kabedi Arsène Mabika Mabika Richard Phillips Michael Frimpong Dorothy Yeboah-Manu Vera Yatta Walker Olaoluwa Akinwale Maman Issaka Gisela Bretzel Kingsley Asiedu Sara Eyangoh A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Buruli ulcer is one of the 20 neglected tropical diseases in the world. This necrotizing hypodermitis is a chronic debilitating disease caused by an environmental Mycobacterium ulcerans. At least 33 countries with tropical, subtropical and temperate climates have reported Buruli ulcer in African countries, South America and Western Pacific regions. Majority of cases are spread across West and Central Africa. The mode of transmission is unclear, hindering the implementation of adequate prevention for the population. Currently, early diagnosis and treatment are crucial to minimizing morbidity, costs and preventing long-term disability. Biological confirmation of clinical diagnosis of Buruli ulcer is essential before starting chemotherapy. Indeed, differential diagnosis are numerous and Buruli ulcer has varying clinical presentations. Up to now, the gold standard biological confirmation is the quantitative PCR, targeting the insertion sequence IS2404 of M. ulcerans performed on cutaneous samples. Due to the low PCR confirmation rate in endemic African countries (under 30% in 2018) for numerous identified reasons within this article, 11 laboratories decided to combine their efforts to create the network "BU-LABNET" in 2019. The first step of the network was to harmonize the procedures and ship specific reagents to each laboratory. With this system in place, implementation of these procedures for testing and follow-up was easy and the laboratories were able to carry out their first quality control with a very high success rate. It is now time to integrate other neglected tropical diseases to this platform, such as yaws or leprosy. |
format |
Article in Journal/Newspaper |
author |
Estelle Marion Numfor Hycenth Sundeep Chaitanya Vedithi Marie Robbe-Saule Valérie Donkeng Line-Marlène Ganlonon Affolabi Dissou Solange Kakou Ngazoa Marie-Jose Kabedi Arsène Mabika Mabika Richard Phillips Michael Frimpong Dorothy Yeboah-Manu Vera Yatta Walker Olaoluwa Akinwale Maman Issaka Gisela Bretzel Kingsley Asiedu Sara Eyangoh |
author_facet |
Estelle Marion Numfor Hycenth Sundeep Chaitanya Vedithi Marie Robbe-Saule Valérie Donkeng Line-Marlène Ganlonon Affolabi Dissou Solange Kakou Ngazoa Marie-Jose Kabedi Arsène Mabika Mabika Richard Phillips Michael Frimpong Dorothy Yeboah-Manu Vera Yatta Walker Olaoluwa Akinwale Maman Issaka Gisela Bretzel Kingsley Asiedu Sara Eyangoh |
author_sort |
Estelle Marion |
title |
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". |
title_short |
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". |
title_full |
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". |
title_fullStr |
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". |
title_full_unstemmed |
A combined effort of 11 laboratories in the WHO African region to improve quality of Buruli ulcer PCR diagnosis: The "BU-LABNET". |
title_sort |
combined effort of 11 laboratories in the who african region to improve quality of buruli ulcer pcr diagnosis: the "bu-labnet". |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010908 https://doaj.org/article/929f63fe7c044fe687ba7eb80d26f10a |
geographic |
Arctic Pacific |
geographic_facet |
Arctic Pacific |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 11, p e0010908 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010908 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010908 https://doaj.org/article/929f63fe7c044fe687ba7eb80d26f10a |
op_doi |
https://doi.org/10.1371/journal.pntd.0010908 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
11 |
container_start_page |
e0010908 |
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1766343332918722560 |