Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1
Abstract Background Antibodies targeting variant antigens expressed on the surface of Plasmodium falciparum infected erythrocytes have been associated with protection from clinical malaria. The precise target for these antibodies is unknown. The best characterized and most likely target is the eryth...
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ftdoajarticles:oai:doaj.org/article:9293902e041a44b7997f95056ff9ddca 2023-05-15T15:13:54+02:00 Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 Pinches Robert Kortok Moses Mwangi Tabitha W Christodoulou Zoe Mackintosh Claire L Williams Thomas N Marsh Kevin Newbold Christopher I 2008-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-155 https://doaj.org/article/9293902e041a44b7997f95056ff9ddca EN eng BMC http://www.malariajournal.com/content/7/1/155 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-155 1475-2875 https://doaj.org/article/9293902e041a44b7997f95056ff9ddca Malaria Journal, Vol 7, Iss 1, p 155 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-155 2022-12-31T08:04:27Z Abstract Background Antibodies targeting variant antigens expressed on the surface of Plasmodium falciparum infected erythrocytes have been associated with protection from clinical malaria. The precise target for these antibodies is unknown. The best characterized and most likely target is the erythrocyte surface-expressed variant protein family Plasmodium falciparum erythrocyte membrane protein 1 ( Pf EMP1). Methods Using recombinant proteins corresponding to five domains of the expressed A4 var gene, A4 Pf EMP1, the naturally occurring antibody response was assessed, by ELISA, to each domain in serum samples obtained from individuals resident in two communities of differing malaria transmission intensity on the Kenyan coast. Using flow cytometry, the correlation in individual responses to each domain with responses to intact A4- infected erythrocytes expressing A4 Pf EMP1 on their surface as well as responses to two alternative parasite clones and one clinical isolate was assessed. Results Marked variability in the prevalence of responses between each domain and between each transmission area was observed, as wasa strong correlation between age and reactivity with some but not all domains. Individual responses to each domain varied strikingly, with some individuals showing reactivity to all domains and others with no reactivity to any, this was apparent at all age groups. Evidence for possible cross-reactivity in responses to the domain DBL4γ was found. Conclusion Individuals acquire antibodies to surface expressed domains of a highly variant protein. The finding of potential cross-reactivity in responses to one of these domains is an important initial finding in the consideration of potential vaccine targets. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Wasa ENVELOPE(-13.408,-13.408,-73.043,-73.043) Malaria Journal 7 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Pinches Robert Kortok Moses Mwangi Tabitha W Christodoulou Zoe Mackintosh Claire L Williams Thomas N Marsh Kevin Newbold Christopher I Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Antibodies targeting variant antigens expressed on the surface of Plasmodium falciparum infected erythrocytes have been associated with protection from clinical malaria. The precise target for these antibodies is unknown. The best characterized and most likely target is the erythrocyte surface-expressed variant protein family Plasmodium falciparum erythrocyte membrane protein 1 ( Pf EMP1). Methods Using recombinant proteins corresponding to five domains of the expressed A4 var gene, A4 Pf EMP1, the naturally occurring antibody response was assessed, by ELISA, to each domain in serum samples obtained from individuals resident in two communities of differing malaria transmission intensity on the Kenyan coast. Using flow cytometry, the correlation in individual responses to each domain with responses to intact A4- infected erythrocytes expressing A4 Pf EMP1 on their surface as well as responses to two alternative parasite clones and one clinical isolate was assessed. Results Marked variability in the prevalence of responses between each domain and between each transmission area was observed, as wasa strong correlation between age and reactivity with some but not all domains. Individual responses to each domain varied strikingly, with some individuals showing reactivity to all domains and others with no reactivity to any, this was apparent at all age groups. Evidence for possible cross-reactivity in responses to the domain DBL4γ was found. Conclusion Individuals acquire antibodies to surface expressed domains of a highly variant protein. The finding of potential cross-reactivity in responses to one of these domains is an important initial finding in the consideration of potential vaccine targets. |
format |
Article in Journal/Newspaper |
author |
Pinches Robert Kortok Moses Mwangi Tabitha W Christodoulou Zoe Mackintosh Claire L Williams Thomas N Marsh Kevin Newbold Christopher I |
author_facet |
Pinches Robert Kortok Moses Mwangi Tabitha W Christodoulou Zoe Mackintosh Claire L Williams Thomas N Marsh Kevin Newbold Christopher I |
author_sort |
Pinches Robert |
title |
Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 |
title_short |
Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 |
title_full |
Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 |
title_fullStr |
Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 |
title_full_unstemmed |
Acquisition of naturally occurring antibody responses to recombinant protein domains of Plasmodium falciparum erythrocyte membrane protein 1 |
title_sort |
acquisition of naturally occurring antibody responses to recombinant protein domains of plasmodium falciparum erythrocyte membrane protein 1 |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-155 https://doaj.org/article/9293902e041a44b7997f95056ff9ddca |
long_lat |
ENVELOPE(-13.408,-13.408,-73.043,-73.043) |
geographic |
Arctic Wasa |
geographic_facet |
Arctic Wasa |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 155 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/155 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-155 1475-2875 https://doaj.org/article/9293902e041a44b7997f95056ff9ddca |
op_doi |
https://doi.org/10.1186/1475-2875-7-155 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
_version_ |
1766344412595486720 |